Endometrial factor testing is a useful tool in clinical infertility management

Simón, Carlos

doi:10.1016/j.rbmo.2021.12.014

Cited by 4 publications

(2 citation statements)

References 38 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Although embryo abnormalities are a major cause of preclinical pregnancy loss [ 1 , 2 , 3 , 4 , 5 ], an increasing number of studies implicate the endometrium as an additional contributing factor in embryo implantation failure and early embryo miscarriage, reviewed in [ 6 , 7 , 8 , 9 , 10 , 11 , 12 ]. Given that assisted reproductive technologies (ARTs) depend on the transfer of healthy embryos into a receptive endometrium, a non-receptive endometrium at the time of embryo transfer is thought to also undercut the full potential of ART-conceived pregnancies [ 13 , 14 , 15 , 16 , 17 ]. In addition, a non-receptive endometrium is implicated as one of a number of factors leading to recurrent pregnancy loss [ 18 , 19 ], defined in the United States as two or more consecutive pregnancy losses that are diagnosed by ultrasound and/or histopathology [ 20 ].…”

Section: Introductionmentioning

confidence: 99%

Establishment of Murine Pregnancy Requires the Promyelocytic Leukemia Zinc Finger Transcription Factor

Hai,

Maurya,

DeMayo

et al. 2024

IJMS

View full text Add to dashboard Cite

Using an established human primary cell culture model, we previously demonstrated that the promyelocytic leukemia zinc finger (PLZF) transcription factor is a direct target of the progesterone receptor (PGR) and is essential for progestin-dependent decidualization of human endometrial stromal cells (HESCs). These in vitro findings were supported by immunohistochemical analysis of human endometrial tissue biopsies, which showed that the strongest immunoreactivity for endometrial PLZF is detected during the progesterone (P4)-dominant secretory phase of the menstrual cycle. While these human studies provided critical clinical support for the important role of PLZF in P4-dependent HESC decidualization, functional validation in vivo was not possible due to the absence of suitable animal models. To address this deficiency, we recently generated a conditional knockout mouse model in which PLZF is ablated in PGR-positive cells of the mouse (Plzf d/d). The Plzf d/d female was phenotypically analyzed using immunoblotting, real-time PCR, and immunohistochemistry. Reproductive function was tested using the timed natural pregnancy model as well as the artificial decidual response assay. Even though ovarian activity is not affected, female Plzf d/d mice exhibit an infertility phenotype due to an inability of the embryo to implant into the Plzf d/d endometrium. Initial cellular and molecular phenotyping investigations reveal that the Plzf d/d endometrium is unable to develop a transient receptive state, which is reflected at the molecular level by a blunted response to P4 exposure with a concomitant unopposed response to 17-β estradiol. In addition to a defect in P4-dependent receptivity, the Plzf d/d endometrium fails to undergo decidualization in response to an artificial decidual stimulus, providing the in vivo validation for our earlier HESC culture findings. Collectively, our new Plzf d/d mouse model underscores the physiological importance of the PLZF transcription factor not only in endometrial stromal cell decidualization but also uterine receptivity, two uterine cellular processes that are indispensable for the establishment of pregnancy.

show abstract

Section: Introductionmentioning

confidence: 99%

Establishment of Murine Pregnancy Requires the Promyelocytic Leukemia Zinc Finger Transcription Factor

Hai,

Maurya,

DeMayo

et al. 2024

IJMS

View full text Add to dashboard Cite

show abstract

“… 4 Both prospective and retrospective studies found that patients with abundant Lactobacillus in endometrial fluid and endometrial tissue samples tended to have successful live births compared with patients with bacteria, such as Streptococcus , and Staphylococcus . 5 Ichiyama et al. reported on the characteristics of the microbiota of the uterine cavity and vagina in cases of recurrent implantation failure (RIF), 6 and Tsai et al.…”

Section: Introductionmentioning

confidence: 99%

Metabolomic and microbiome analysis of cervicovaginal mucus in in vitro fertilization–embryo transfer: Toward predicting pregnancy success

Nishio,

Iwata,

Kawasaki

et al. 2024

Reprod Medicine & Biology

View full text Add to dashboard Cite

PurposeIn the context of in vitro fertilization–embryo transfer (IVF–ET), factors other than egg quality may be key determinants of treatment success, in particular, maternal factors related to uterine endometrial receptivity and unidentified factors. We therefore aimed to analyze the metabolome and microbiome in IVF–ET patients who did and did not achieve pregnancy.MethodsCervicovaginal mucus was collected from patients undergoing IVF–ET. Metabolite analysis was conducted by liquid chromatography‐mass spectrometry and the microbiota were determined by the polymerase chain reaction using universal 16S‐rRNA gene bacterial primers by MiSeq sequencing. Patients were classified as pregnant (N = 10) or nonpregnant (N = 13). Metabolic pathways were examined by MetaboAnalyst.ResultsThree metabolic pathways, including alanine‐aspartate–glutamate metabolism, arginine biosynthesis, and cysteine‐methionine metabolism, were commonly decreased at the time of embryo transfer irrespective pregnant outcomes. Notably, pyruvate was decreased in the pregnant group. Amino acid metabolites showed inverse correlations with the presence of anaerobic microbiota in the nonpregnant group.ConclusionsMetabolism decreased during embryo transplantation, with a notable decrease in pyruvate metabolism, particularly in patients who became pregnant. The behavior of metabolites in the pregnant and nonpregnant groups suggests that metabolome analysis in the cervicovaginal mucus may be a diagnostic marker for predicting pregnancy.

show abstract