Endometrial Perivascular Progenitor Cells and Uterus Regeneration

Li, Shiyuan; Ding, Lijun

doi:10.3390/jpm11060477

Cited by 23 publications

(32 citation statements)

References 94 publications

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“…Other markers of perivascular eMSC include NG2, Stro-1, EphA3, W8B2, and CD271 and CD34 which are found in the adventitia of blood vessels rather than a pericyte or medial location [reviewed in ( 48 , 56 )]. However, the CD34 population failed to regenerate human endometrium in functional studies ( 57 ).…”

Section: Human Stem/progenitor Cells In Menstruation Endometrial Repa...mentioning

confidence: 99%

Endometrial Stem/Progenitor Cells–Their Role in Endometrial Repair and Regeneration

Cousins

Filby

Gargett

2022

Front. Reprod. Health

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The human endometrium is a remarkable tissue, undergoing ~450 cycles of proliferation, differentiation, shedding (menstruation), repair, and regeneration over a woman's reproductive lifespan. Post-menstrual repair is an extremely rapid and scar-free process, with re-epithelialization of the luminal epithelium completed within 48 h of initiation of shedding. Following menstruation, the functionalis grows from the residual basalis layer during the proliferative phase under the influence of rising circulating estrogen levels. The regenerative capacity of the endometrium is attributed to stem/progenitor cells which reside in both the epithelial and stromal cell compartments of the basalis layer. Finding a definitive marker for endometrial epithelial progenitors (eEPCs) has proven difficult. A number of different markers have been suggested as putative progenitor markers including, N-cadherin, SSEA-1, AXIN2, SOX-9 and ALDH1A1, some of which show functional stem cell activity in in vitro assays. Each marker has a unique location(s) in the glandular epithelium, which has led to the suggestion that a differentiation hierarchy exists, from the base of epithelial glands in the basalis to the luminal epithelium lining the functionalis, where epithelial cells express different combinations of markers as they differentiate and move up the gland into the functionalis away from the basalis niche. Perivascular endometrial mesenchymal stem cells (eMSCs) can be identified by co-expression of PDGFRβ and CD146 or by a single marker, SUSD2. This review will detail the known endometrial stem/progenitor markers; their identity, location and known interactions and hierarchy across the menstrual cycle, in particular post-menstrual repair and estrogen-driven regeneration, as well as their possible contributions to menstruation-related disorders such as endometriosis and regeneration-related disorder Asherman's syndrome. We will also highlight new techniques that allow for a greater understanding of stem/progenitor cells' role in repair and regeneration, including 3D organoids, 3D slice cultures and gene sequencing at the single cell level. Since mouse models are commonly used to study menstruation, repair and regeneration we will also detail the mouse stem/progenitor markers that have been investigated in vivo.

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Section: Human Stem/progenitor Cells In Menstruation Endometrial Repa...mentioning

confidence: 99%

Endometrial Stem/Progenitor Cells–Their Role in Endometrial Repair and Regeneration

Cousins

Filby

Gargett

2022

Front. Reprod. Health

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show abstract

“…Both cell types share similar adherence and morphology, as well as being localized perivascularly. Additionally, they both possess similar cell markers, such as CD146, PDGFR, CD29, CD44, CD105, CD73, and CD90 [ 25 , 26 ]. Furthermore, like with uterine stromal stem cells, CD146 + PDGFRβ + pericytes can be induced to differentiate into multiple mesodermal lineages, such as adipogenic, osteogenic, and neuronal lineages.…”

Section: Introductionmentioning

confidence: 99%

“…Indeed, CD146 + pericytes, localized around capillaries and micro-vessels, within both endometrial functionalis and basalis layers [ 27 ] can re-constitute endometrial stromal tissues in vivo [ 28 ]. Therefore, pericytes are considered as the origin of endometrial stromal stem cells [ 26 ].…”

Section: Introductionmentioning

confidence: 99%

Uterus: A Unique Stem Cell Reservoir Able to Support Cardiac Repair via Crosstalk among Uterus, Heart, and Bone Marrow

Hatta

Yao

2022

Cells

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Clinical evidence suggests that the prevalence of cardiac disease is lower in premenopausal women compared to postmenopausal women and men. Although multiple factors contribute to this difference, uterine stem cells may be a major factor, as a high abundance of these cells are present in the uterus. Uterine-derived stem cells have been reported in several studies as being able to contribute to cardiac neovascularization after injury. However, our studies uniquely show the presence of an “utero-cardiac axis”, in which uterine stem cells are able to home to cardiac tissue to promote tissue repair. Additionally, we raise the possibility of a triangular relationship among the bone marrow, uterus, and heart. In this review, we discuss the exchange of stem cells across different organs, focusing on the relationship that exists between the heart, uterus, and bone marrow. We present increasing evidence for the existence of an utero-cardiac axis, in which the uterus serves as a reservoir for cardiac reparative stem cells, similar to the bone marrow. These cells, in turn, are able to migrate to the heart in response to injury to promote healing.

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“… 15–17 The vascularity in the endometrium affects endometrial receptivity to the blastocyst during the “implantation window”. 18 , 19 Then, the endometrium grows thicker as the vessel gradually grows mature, implicating sufficient endometrial receptivity for embryo implantation. 19 …”

Section: Introductionmentioning

confidence: 99%

“…[15][16][17] The vascularity in the endometrium affects endometrial receptivity to the blastocyst during the "implantation window". 18,19 Then, the endometrium grows thicker as the vessel gradually grows mature, implicating sufficient endometrial receptivity for embryo implantation. 19 Many researchers have studied the endometrial molecular expression in humans via endometrial biopsy during the "implantation window".…”

Section: Introductionmentioning

confidence: 99%

Ultrasound Molecular Imaging of Neovascularization for Evaluation of Endometrial Receptivity Using Magnetic iRGD-Modified Lipid-Polymer Hybrid Microbubbles

He¹,

Zhou²,

Li³

et al. 2022

IJN

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Background Angiogenesis plays an important role in endometrial receptivity, determining the response of the endometrium to the blastocyst at the early stage of embryo implantation. During the application of assisted reproduction technologies, it is very important to evaluate the status of uterine angiogenesis before deciding on embryo implantation. Targeted microbubbles (MBs)-based ultrasound molecular imaging (UMI) can noninvasively detect the expression status of biomarkers at the molecular level, thereby being a potential diagnosis strategy for various diseases and their therapeutic evaluation. Methods The iRGD-lipopeptide (DSPE-PEG2000-iRGD) conjugate was prepared with iRGD peptides and DSPE-PEG2000-maleimide through the Michael-type addition reaction. Then, the magnetic iRGD-modified lipid-polymer hybrid MBs (Mag-iLPMs) were prepared with the double-emulsification-solvent-evaporation method. Magnetic targeting of Mag-iLPMs was confirmed under the microscope, followed by a rectangular magnet. Next, the in vitro targeted binding of MBs to murine brain-derived endothelial cells.3 (bEnd.3) and human umbilical vein endothelial cells (HUVEC) were evaluated. The ratio of MBs binding to bEnd.3 and HUVEC at the same field was also compared. For in vivo studies, bolus injections of targeted or control MBs were randomly administrated to non-pregnant or pregnant rats on day 5. Then, the uteri were imaged using a VisualSonics Vevo 2100 ultrasound system (Fujifilm VisualSonics Inc., Ontario, Canada) equipped with a high-frequency transducer. Ultrasonic imaging signals were acquired from Mag-iLPMs, and compared with Mag-LPMs, iLPMs, and LPMs. Results The Mag-iLPMs showed excellent performance in ultrasound contrast imaging and binding affinity to target cells. Using the magnetic field, 10.5- and 12.47-fold higher binding efficiency to bEnd.3 and HUVEC were achieved compared to non-magnetic iLPMs, respectively. Significantly enhanced UMI signals were also observed in the uteri of rats intravenously injected pregnant rats (6.58-fold higher than rats injected with iLPMs). Conclusion We provided a powerful ultrasonic molecular functional imaging tool for uterine angiogenesis evaluation before embryonic implantation.

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Endometrial Perivascular Progenitor Cells and Uterus Regeneration

Cited by 23 publications

References 94 publications

Endometrial Stem/Progenitor Cells–Their Role in Endometrial Repair and Regeneration

Endometrial Stem/Progenitor Cells–Their Role in Endometrial Repair and Regeneration

Uterus: A Unique Stem Cell Reservoir Able to Support Cardiac Repair via Crosstalk among Uterus, Heart, and Bone Marrow

Ultrasound Molecular Imaging of Neovascularization for Evaluation of Endometrial Receptivity Using Magnetic iRGD-Modified Lipid-Polymer Hybrid Microbubbles

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