Endometriosis induces gut microbiota alterations in mice

Yuan, Man; Dong, Li; Zhang, Zhe; Sun, Hongtao; An, Min Sung; Wang, Guoyun

doi:10.1093/humrep/dex372

Cited by 121 publications

(141 citation statements)

References 57 publications

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“…Five of the eighteen studies identified were conducted using animal models. One study involved the use of rhesus monkeys as a non‐human primate model, whereas the remaining four studies used murine or rodent models . This included Sprague‐Dawley and C57BL models; however, one study did not declare which murine model was used .…”

Section: Resultsmentioning

confidence: 99%

“…One study involved the use of rhesus monkeys as a non‐human primate model, whereas the remaining four studies used murine or rodent models . This included Sprague‐Dawley and C57BL models; however, one study did not declare which murine model was used . Endometriosis was surgically induced in the murine/rodent models via intraperitoneal transplantation; however, there was slight variation among the methods used in each study.…”

Section: Resultsmentioning

confidence: 99%

“…The various kits and sequencing methods used are outlined in Table . Of the seven studies that used 16S rRNA amplicon sequencing, only five discussed the targeted sequencing region . Each study targeted different regions, including region V3/V4, region V4, region V3–V5, region V4/V5 and region V5/V6 .…”

Section: Resultsmentioning

confidence: 99%

“…α ‐Diversity is used to describe the diversity of a microbial community within a single sample or site whereas β ‐diversity is an index used to compare the diversity of microbial communities across different samples or sites . Five of eighteen studies assessed α ‐diversity . Three studies used Shannon's Diversity Index to assess α ‐diversity .…”

Section: Resultsmentioning

confidence: 99%

“…Five of eighteen studies assessed α ‐diversity . Three studies used Shannon's Diversity Index to assess α ‐diversity . The study by Yuan et al .…”

Section: Resultsmentioning

confidence: 99%

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Endometriosis and the microbiome: a systematic review

Leonardi

Hicks

El‐Assaad

et al. 2019

BJOG

115

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Background The aetiology and pathogenesis of endometriosis are still under investigation. There is evidence that there is a complex bidirectional interaction between endometriosis and the microbiome.Objective To systematically review the available literature on the endometriosis-microbiome interaction, with the aim of guiding future inquiries in this emerging area of endometriosis research.Search strategy MEDLINE, Embase, Scopus and Web of Science were searched through May 2019. A manual search of reference lists of relevant studies was also performed.Selection criteria Published and unpublished literature in any language describing a comparison of the microbiome state in mammalian hosts with and without endometriosis.Data collection and analysis Identified studies were screened and assessed independently by two authors. Data were extracted and compiled in a qualitative synthesis of the evidence.Main results Endometriosis appears to be associated with an increased presence of Proteobacteria, Enterobacteriaceae, Streptococcus spp. and Escherichia coli across various microbiome sites. The phylum Firmicutes and the genus Gardnerella also appear to have an association; however, this remains unclear.Conclusions The complex bidirectional relationship between the microbiome and endometriosis has begun to be characterised by the studies highlighted in this systematic review. Laboratory and clinical studies demonstrate that there are indeed differences in the microbiome composition of hosts with and without endometriosis.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

“…Five of eighteen studies assessed α ‐diversity . Three studies used Shannon's Diversity Index to assess α ‐diversity . The study by Yuan et al .…”

Section: Resultsmentioning

confidence: 99%

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Endometriosis and the microbiome: a systematic review

Leonardi

Hicks

El‐Assaad

et al. 2019

BJOG

115

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Enterolactone and trabectedin suppress epithelial ovarian cancer synergistically via upregulating THBS1

Zhang

Lin

Zhang

et al. 2023

Phytotherapy Research

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Epithelial ovarian cancer (EOC) is the most common and fatal subtype of ovarian malignancies, with no effective therapeutics available. Our previous studies have demonstrated extraordinary suppressive efficacy of enterolactone (ENL) on EOC. A chemotherapeutic agent, trabectedin (Trabe), is shown to be effective on ovarian cancer, especially when combined with other therapeutics, such as pegylated liposomal doxorubicin or oxaliplatin. Thrombospondin 1 (THBS1), a kind of matrix glycoprotein, plays important roles against cancer development through inhibiting angiogenesis but whether it is involved in the suppression of EOC by ENL or Trabe remains unknown. To test combined suppressive effects of ENL and Trabe on EOC and possible involvement of THBS1 in the anticancer activities of ENL and Trabe. The EOC cell line ES‐2 was transfected with overexpressed THBS1 by lentivirus vector. We employed tube formation assay to evaluate the anti‐angiogenesis activity of ENL and of its combined use with Trabe after THBS1 overexpression and established drug intervention and xenograft nude mouse cancer models to assess the in vivo effects of the hypothesized synergistic suppression between the agents and the involvement of THBS1. Mouse fecal samples were collected for 16S rDNA sequencing and microbiota analysis. We detected strong inhibitory activities of ENL and Trabe against the proliferation and migration of cancer cells and observed synergistic effects between ENL and Trabe in suppressing EOC. ENL and Trabe, given either separately or in combination, could suppress the tube formation capability of human microvascular endothelial cells, and this inhibitory effect became even stronger with THBS1 overexpression. In the ENL plus Trabe combination group, the expression of tissue inhibitor of metalloproteinases 3 and cluster of differentiation 36 was both upregulated, whereas matrix metalloproteinase 9, vascular endothelial growth factor, and cluster of differentiation 47 were all decreased. With the overexpression of THBS1, the results became even more pronounced. In animal experiments, combined use of ENL and Trabe showed superior inhibitory effects to either single agent and significantly suppressed tumor growth, and the overexpression of THBS1 further enhanced the anti‐cancer activities of the drug combination group. ENL and Trabe synergistically suppress EOC and THBS1 could remarkably facilitate the synergistic anticancer effects of ENL and Trabe.

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Microbiome analysis in women with endometriosis: Does a microbiome exist in peritoneal fluid and ovarian cystic fluid?

Oishi

Mekaru

Tanaka³

et al. 2022

Reprod Medicine & Biology

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Purpose To investigate the relationship between the microbiome of the female genital tract and endometriosis. Methods This prospective cohort study included 36 women who underwent laparoscopic surgery for ovarian tumor from July 2019 to April 2020. Of them, 18 had endometriosis, and 18 did not have endometriosis. Vaginal secretions, endometrial fluid, peritoneal fluid, and ovarian cystic fluid were collected during surgery. Next‐generation sequencing of bacterial 16S rRNA was performed to characterize the microbiome. Results Specific microbiomes were not detected in either peritoneal fluid or ovarian cystic fluid regardless of the presence or absence of endometriosis and the type of cyst. When the cutoff value of infectious bacterial abundance in the vagina was set as 64.3%, there were many cases more than a cutoff value in the endometriosis group significantly ( p = 0.01). When the cutoff value of infectious bacterial abundance in the endometrium was set as 18.6%, there were many cases more than a cutoff level in the endometriosis cases significantly ( p = 0.02). Conclusion Peritoneal fluid and ovarian cystic fluid are almost sterile, although dysbiosis may occur in the vaginal and endometrial microbiome in women with endometriosis.

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Endometriosis induces gut microbiota alterations in mice

Abstract: This research was supported by Grant (81571417) from the National Science Foundation of China and Grant (2015GSF118092) from the Technology Development Plan of Shandong Province. The authors report no conflict of interest.

Cited by 121 publications

References 57 publications

Endometriosis and the microbiome: a systematic review

Endometriosis and the microbiome: a systematic review

Enterolactone and trabectedin suppress epithelial ovarian cancer synergistically via upregulating THBS1

Microbiome analysis in women with endometriosis: Does a microbiome exist in peritoneal fluid and ovarian cystic fluid?

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