Endometrite Subclínica Após O Tratamento De Vacas Com Endometrite Clínica

Moura, Ana Rita Ferreira; Tsuruta, Suzana Akemi; Oliveira, Patrícia Magalhães de; Nasciutti, Nayara Resende; Santos, Ricarda Maria dos; Saut, João Paulo Elsen

doi:10.5380/avs.v17i3.25529

Cited by 3 publications

(2 citation statements)

References 36 publications

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“…Collection of uterine contents was performed as described by Martins et al (19), which consisted of collecting samples using a human gynecological brush coupled to a universal semen applicator with a stainless steel rod, with protection by a French sheath and sanitary shirt. Then, after passing through the cervix and reaching the base of the uterine body, the sanitary liner was broken, and the applicator plunger was pushed forwards, exposing the brush, which was in contact with the endometrium, was rotated clockwise, and then removed as described by Moura et al (20). Subsequently, smears with light circular movements were performed on slides with the uterine content obtained with the Cytobrush R cervical brush (Kolplast, Itupeva, Brazil), and subsequently, the prepared slides were stained with panoptic fast stain for evaluation of cellularity by optical microscopy (Primo Star 1, Carl Zeiss, São Paulo, Brazil).…”

Section: Endometrial Cytologymentioning

confidence: 99%

Brief Research Report: Expression of PD-1 and CTLA-4 in T Lymphocytes and Their Relationship With the Periparturient Period and the Endometrial Cytology of Dairy Cows During the Postpartum Period

et al. 2022

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The present study sought to evaluate the expression of PD-1 and CTLA-4 in blood T lymphocytes during the periparturient period and their relationship with uterine health in dairy cows, as determined by endometrial cytology and serum concentrations of β-hydroxybutyrate (BHB) and non-esterified fatty acids (NEFAs), which are indicators of a negative energy balance. The second objective of this study was to investigate whether the expression of PD-1 and CTLA-4 in T lymphocytes is associated with the serum acute phase-protein haptoglobin concentration during the periparturient period. To address these objectives, 26 clinically healthy dairy cows were used. Peripheral blood was collected 14 days prepartum (T-14), at calving (T0), and 30 days postpartum (T30) to measure the expression of PD-1 and CTLA-4 in blood T lymphocytes by flow cytometry. In addition, we collected blood at T0, 10 days after parturition (T10), and T30 to obtain serum and determine the serum concentrations of NEFA, BHB, and Hp. Endometrial cytology was performed at T10, 20 days after parturition (T20), and T30. In the present study, we observed higher expression of CTLA-4 and PD-1 in T lymphocytes at parturition and in the prepartum period, which could indicate a relationship between these immune checkpoints and immunological tolerance during gestation in dairy cattle. In addition, a negative association between the expression of these immune checkpoints prepartum or at parturition and endometrial cytology at T20 and T30 was observed, indicating the negative implications of these immune response regulators in susceptibility to infections. This finding was further corroborated by the relationship between the serum concentration of haptoglobin and the expression of CTLA-4 and PD-1 by T lymphocytes. However, we did not observe a relationship between the indicators of negative energy balance, evaluated by the serum concentrations of BHB and NEFA, and the expression of the immune checkpoint markers studied. Thus, our findings represent an initial step that paves the way for the development of new therapeutic alternatives directed by the host with the objective of increasing the resistance of dairy cattle to infections in this critical period of life.

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Section: Endometrial Cytologymentioning

confidence: 99%

Brief Research Report: Expression of PD-1 and CTLA-4 in T Lymphocytes and Their Relationship With the Periparturient Period and the Endometrial Cytology of Dairy Cows During the Postpartum Period

et al. 2022

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“…During this period, cow undergoes metabolic and hormonal changes, which produce immunosuppression with the predisposition to endometrium inflammation, bacterial infection and delayed in uterine involution [8,13]. This limits the development of the embryo in uterine lumen, causing infertility, prolonged calving -calving intervals, lactations with long periods and economic losses [12].…”

Section: Introductionmentioning

confidence: 99%

Occurrence of Subclinical Endometritis in Dairy Cattle and Effect on Reproductive Efficiency

Vallejo

Chaves

Benavides

et al. 2018

Acta Scientiae. Vet.

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Background: Subclinical endometritis (SE) have a negative impact on fertility due to an absence of clinical signs which difficult its diagnosis and treatment. The prevalence and impact of the disease on the reproductive status of dairy herds is not known in the region, the objective of the present study was to determine the prevalence of the disease and its effect on the reproductive efficiency in cows of the dairy area from Putumayo State, Colombia.Materials, Methods & Results: Cross-sectional study was made of 166 dairy cows from 30 days postpartum in the municipalities: Santiago, Sibundoy, Colon, and San Francisco. Reproductive evaluation and endometrial cytology were made to establish the SE prevalence determining subsequently the number of open days. Cow whit more than 120 days in milk (DIM) and without confirmed pregnancy was defined as “not reproductive efficiency”. Association between SE and reproductive efficiency was established through Odds Ratio from contingency tables. Bias and confusion control was made through stratified analysis. Results showed 32 cows without changes in reproductive clinical evaluation and inflammatory changes in cytological evaluation (PMNn >5%) for a SE prevalence of 19.27%. Disease occurs most frequently in Colón (23.10%) but the frequency of the disease was not different among the regions (P > 0.05). The group of animals with the greatest days open (DO) mean (161 DIM) had a normal ovarian function and subclinical endometritis. The 6.6% of cows had a poor reproductive prognosis (subclinical endometritis, anestrous and 144 DIM). For the stratified analysis (controlling by anestrus) was estimated the crude OR (OR 5.93; P < 0.05; CI 95% 2.56-14.6) and adjusted ORMH (OR 5.78; P < 0.05; CI 95% 2.39-13.9). Difference between adjusted ORMH and crude OR (3%) and Wald X2 test (P > 0.05) suggesting that there is no confusion and allowed to establish that “the odds of low reproductive efficiency (Days open >120) increases 5.9 times when cows suffer subclinical endometritis, than when do not have the disease”.Discussion: Study prevalence was lower than the reported by other authors. Variability of the results by the different authors ratifies the multifactorial characteristic of the disease and thr differences in the immune response of the animals. Cows diagnosed with SE, normal ovarian function and 161 DIM shown that the disease increases significantly the open days of the herd reducing reproductive efficiency. These findings are consistent with other authors who report that subclinical endometritis: increased open days and reduced conception rates and increased the risk of no pregnancy at 150 days postpartum. In the study, cows with subclinical endometritis and normal ovarian function had open days mean lower to 120 and more probability to have a good reproductive performance if pregnancy is achieved, but the dairy herds of the region lack of adequate systems of detection of estro and services and have no systems that allow the diagnosis of SE and anoestrus. The 69% of the evaluated animals present some clinical characteristics (SE, anoestrous) that predispose them to low reproductive behaviour. It is recommended to routinely use endometrial cytology to diagnose SE and improving the reproductive efficiency of animals.

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Endometritis subclínica diagnosticada mediante cytobrush y comportamiento reproductivo en vacas del municipio de Pupiales, Colombia

et al. 2014

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Objetivo: determinar la distribución de endometritis subclínica y el comportamiento reproductivo de vacas lecheras diagnosticadas mediante citología endometrial, del municipio de Pupiales, Nariño. Materiales y métodos: se realizó evaluación reproductiva (palpación rectal, ultrasonografía y evaluación del mucus vaginal) para determinar la ausencia de gestación temprana y de signos clínicos de infección uterina, y mediante cateterismo cervical y la técnica cytobrush se muestrearon 174 vacas a partir de los 30 días posparto. Posteriormente, se organizó la población objeto de estudio con base en su función ovárica (vacía ciclando normal o en anestro) y el estatus del útero (con presencia de endometritis o sin esta). Resultados: 43 vacas (24,7 %) presentaron cambios celulares indicativos de un proceso inflamatorio activo originado en endometrio. El 79,06 % de los animales diagnosticados con endometritis subclínica estaban cíclicas y tenían un bajo desempeño reproductivo. Conclusión: se determinó el alto impacto que tiene la endometritis subclínica en la fertilidad y en la eficiencia reproductiva. Se debe realizar una evaluación reproductiva temprana en conjunto con pruebas que permitan determinar la presencia de afecciones subclínicas concomitantes.

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Endometrite Subclínica Após O Tratamento De Vacas Com Endometrite Clínica

Cited by 3 publications

References 36 publications

Brief Research Report: Expression of PD-1 and CTLA-4 in T Lymphocytes and Their Relationship With the Periparturient Period and the Endometrial Cytology of Dairy Cows During the Postpartum Period

Brief Research Report: Expression of PD-1 and CTLA-4 in T Lymphocytes and Their Relationship With the Periparturient Period and the Endometrial Cytology of Dairy Cows During the Postpartum Period

Occurrence of Subclinical Endometritis in Dairy Cattle and Effect on Reproductive Efficiency

Endometritis subclínica diagnosticada mediante cytobrush y comportamiento reproductivo en vacas del municipio de Pupiales, Colombia

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