Endopeptidase inhibition attenuates the contraction induced by big endothelin‐1 of isolated human penile erectile tissue

Shahin, P.; Rahardjo, Harrina Erlianti; Bannowsky, A.; Tsikas, Dimitrios; Sohn, Michael; Kuczyk, Markus A.; Ückert, Stefan

doi:10.1111/and.13008

Cited by 3 publications

(2 citation statements)

References 37 publications

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“…Penile erectile tissue (corpus cavernosum) was obtained from 5 individuals who had been subjected to gender reassignment surgery (male-to-female). Tissue bath experiments were conducted according to standard procedures, as has been described earlier [18,19]. A passive tension of 0.5 gr (5 mN) was applied to the strip preparations, followed by a resting period of 60 min.…”

Section: Tissue Bath Studies (Contraction-response Studies)mentioning

confidence: 99%

Courses of Arginine-Vasopressin in the Systemic and Cavernous Blood through Different Stages of Sexual Arousal in Healthy Males and Patients with Erectile Dysfunction

et al. 2023

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To investigate the role of the peptide arginine-vasopressin (AVP) in controlling the function of penile erectile tissue, we determined the course of AVP through different stages of sexual arousal in both the systemic and cavernous blood of healthy males and patients presenting with ED. Twenty-five healthy males and 45 patients with ED were exposed to erotic stimulation to induce sexual arousal. Blood was withdrawn from the corpus cavernosum and a cubital vein during penile flaccidity, tumescence, rigid erection (attained only by the healthy individuals), and detumescence. AVP (ng/l plasma) was determined by means of a radioimmunoassay. Effects of AVP (0.1 to 100 nM) on isolated human CC were examined using a tissue bath system. AVP elicited contraction of isolated CC. In the healthy subjects, a decline in AVP levels (5.4 to 3 ng/l) was seen in the systemic blood when the flaccid penis became rigid. In the cavernous blood, no alterations were registered. In the group of ED patients, AVP in the systemic circulation did not display a transient decline. The drop in systemic AVP in healthy males during sexual stimulation might be a prerequisite to enable penile erection.

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Section: Tissue Bath Studies (Contraction-response Studies)mentioning

confidence: 99%

Courses of Arginine-Vasopressin in the Systemic and Cavernous Blood through Different Stages of Sexual Arousal in Healthy Males and Patients with Erectile Dysfunction

et al. 2023

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“…Comparatively, the mechanism of action of CNP is diverse and researchers in the last decade showed that CNP affects coronary blood flow regulation electrophysiology, vascular integrity, endothelial cell and smooth muscle proliferation, angiogenesis, leukocyte activation, and vascular tone ( Perez-Ternero et al ., 2019 ). In the male reproductive system, several studies showed that testicular function and fertility and regulation of erectile tissue can be regulated by CNP ( Hannema et al ., 2013 ; Shahin et al ., 2018 ; Ückert et al ., 2019 ).…”

Section: Introductionmentioning

confidence: 99%

Efficiency of C-type natriuretic peptide on improvement of Iraqi local ram’s epididymal sperms

Khadim,

Zaid

2024

Open Vet J

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Background: Fertility plays great role in animal reproduction since high quality semen improves sheep industry reproduction. The current worldwide data revealed the closely related of CNP to reproductive function of rams. Aims: Evaluation the effect of CNP on cooled sperms using the traditional and molecular assays. Methods: Totally, 20 testicular samples were collected, processed to obtain the semen samples and divided into two parts; one treated with the suitable dose of CNP and the otherserved as a control. Sperm samples of both groups were cooled for 3 days and tested at 0 0h, 24h, 48h and 72h. Results: The findings revealed that the suitable dose of CNP-treated sperms was 0.0110-13. Values individual motility, live sperms and sperm concentration were reduced significantly in CNP-24h, CNP-48h and CNP-72h when compared to control; however, abnormal sperms were increased in both control and CNP groups at 24h, 48h and 72h when compared to values of 0h. Concerning turbidmetric analysis, significant reduction in values of lag time was observed in CNP when compared to control at all times of cooling intervals. In both CNP and control groups, motility index was decreased at 24h, 48h and 72h when compared to 0h. For velocity, significant increases were showed in CNP compared with control at all cooling intervals. However, values of both groups were increased significantly at 24h, 48h and 72h times when compared to 0h. FRMS of CNP was elevated at 0h and decreased at 24h, 48h and 72h when compared to control. Expression of hNPR-B gene was reduced gradually in sperms of CNP and control groups at times of cooling intervals. Conclusion: For our knowledge, this first Iraqi study targets the effect of CNP on epididymal sperms of rams. However, changes occur after excessive CNP exposure remains unclear, and toxicological profile of CNP required furthermore supplements.

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Inhibition of the RIP3/MLKL/TRPM7 necroptotic pathway ameliorates diabetes mellitus-induced erectile dysfunction by reducing cell death, fibrosis, and inflammation

Niu,

Yang,

Zhu

et al. 2024

Front. Pharmacol.

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Diabetes mellitus-induced erectile dysfunction (DMED) is a common complication in patients with diabetes mellitus. Necroptosis is regarded as a form of cell death that is intimately associated with the inflammatory response, which is not only initiated by inflammatory factors such as TNF-α, but also triggers the inflammatory cascade through the rupture of the dying cell. There is no definitive study on the role of necroptosis in the pathological process of DMED. In light of the pathological features of high inflammation levels in DMED patients, we assessed whether the necroptosis plays an important role in the course of DMED. Our study revealed that penile tissues of DMED rats showed high levels of key necroptosis factors such as receptor-interacting protein kinase 3 (RIP3), mixed-lineage kinase domain-like protein (MLKL), and transient receptor potential melatonin 7 (TRPM7). Furthermore, the inhibition of necroptosis with a receptor-interacting protein kinase 3 (RIP3) inhibitor or Yimusake (a common herbal remedy for ED) effectively rescued damage to corpus cavernosum smooth muscle cells (CCSMC) under high glucose conditions. Our findings suggest that inhibition of the RIP3/MLKL/TRPM7 necroptotic pathway could effectively ameliorate CCSMCs fibrosis and death induced by high glucose and inhibited the inflammatory response.

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Endopeptidase inhibition attenuates the contraction induced by big endothelin‐1 of isolated human penile erectile tissue

Cited by 3 publications

References 37 publications

Courses of Arginine-Vasopressin in the Systemic and Cavernous Blood through Different Stages of Sexual Arousal in Healthy Males and Patients with Erectile Dysfunction

Courses of Arginine-Vasopressin in the Systemic and Cavernous Blood through Different Stages of Sexual Arousal in Healthy Males and Patients with Erectile Dysfunction

Efficiency of C-type natriuretic peptide on improvement of Iraqi local ram’s epididymal sperms

Inhibition of the RIP3/MLKL/TRPM7 necroptotic pathway ameliorates diabetes mellitus-induced erectile dysfunction by reducing cell death, fibrosis, and inflammation

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