Endophyte mediated activation of defense enzymes in banana plants pre-immunized with covert endophytes

Savani, Ajit Kumar; Bhattacharyya, Ashok; Baruah, Ananya

doi:10.1007/s42360-020-00245-8

Cited by 18 publications

(7 citation statements)

References 21 publications

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“…In this context, changes in the plant-associated microbiome due to the introduction of a BCA and the identification of genes associated to Foc resistance/tolerance would also help in designing better strategies for FWB management ( Zorrilla-Fontanesi et al, 2020 ). Several studies have reported that BCAs are able to trigger banana plant defense responses against Foc which are associated with the control of FWB ( Fishal et al, 2010 ; Thangavelu et al, 2016 ; Savani et al, 2020 ). However, the mechanisms involved in the control of FWB by P. simiae PICF7 still remains to be elucidated.…”

Section: Introductionmentioning

confidence: 99%

Impacts of the Biocontrol Strain Pseudomonas simiae PICF7 on the Banana Holobiont: Alteration of Root Microbial Co-occurrence Networks and Effect on Host Defense Responses

et al. 2022

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The impact of the versatile biocontrol and plant-growth-promoting rhizobacteria Pseudomonas simiae PICF7 on the banana holobiont under controlled conditions was investigated. We examine the fate of this biological control agent (BCA) upon introduction in the soil, the effect on the banana root microbiota, and the influence on specific host genetic defense responses. While the presence of strain PICF7 significantly altered neither the composition nor the structure of the root microbiota, a significant shift in microbial community interactions through co-occurrence network analysis was observed. Despite the fact that PICF7 did not constitute a keystone, the topology of this network was significantly modified—the BCA being identified as a constituent of one of the main network modules in bacterized plants. Gene expression analysis showed the early suppression of several systemic acquired resistance and induced systemic resistance (ISR) markers. This outcome occurred at the time in which the highest relative abundance of PICF7 was detected. The absence of major and permanent changes on the banana holobiont upon PICF7 introduction poses advantages regarding the use of this beneficial rhizobacteria under field conditions. Indeed a BCA able to control the target pathogen while altering as little as possible the natural host-associated microbiome should be a requisite when developing effective bio-inoculants.

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Section: Introductionmentioning

confidence: 99%

Impacts of the Biocontrol Strain Pseudomonas simiae PICF7 on the Banana Holobiont: Alteration of Root Microbial Co-occurrence Networks and Effect on Host Defense Responses

et al. 2022

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“…Some species of Flavobacterium genus could increase biofilm formation of biocontrol Bacillus velezensis and elevate the transcription of plant defense gene to improve the control efficiency of tomato bacterial wilt [ 57 ]. In addition, Sphingobacterium tabacisoli showed the first line of defense against Fusarium wilt by accumulating defense enzymes such as peroxidase, and polyphenol oxidase and triggering systemic resistance of banana [ 58 ]. Hence, when faced with pathogen invasion, the recruitment of beneficial microbial strains by plants may reduce the harm the disease causes [ 8 ].…”

Section: Discussionmentioning

confidence: 99%

Bacterial wilt affects the structure and assembly of microbial communities along the soil-root continuum

Liang,

Wei,

Song

et al. 2024

Environmental Microbiome

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Background Beneficial root-associated microbiomes play crucial roles in enhancing plant growth and suppressing pathogenic threats, and their application for defending against pathogens has garnered increasing attention. Nonetheless, the dynamics of microbiome assembly and defense mechanisms during pathogen invasion remain largely unknown. In this study, we aimed to investigate the diversity and assembly of microbial communities within four niches (bulk soils, rhizosphere, rhizoplane, and endosphere) under the influence of the bacterial plant pathogen Ralstonia solanacearum. Results Our results revealed that healthy tobacco plants exhibited more diverse community compositions and more robust co-occurrence networks in root-associated niches compared to diseased tobacco plants. Stochastic processes (dispersal limitation and drift), rather than determinism, dominated the assembly processes, with a higher impact of drift observed in diseased plants than in healthy ones. Furthermore, during the invasion of R. solanacearum, the abundance of Fusarium genera, a known potential pathogen of Fusarium wilt, significantly increased in diseased plants. Moreover, the response strategies of the microbiomes to pathogens in diseased and healthy plants diverged. Diseased microbiomes recruited beneficial microbial taxa, such as Streptomyces and Bacilli, to mount defenses against pathogens, with an increased presence of microbial taxa negatively correlated with the pathogen. Conversely, the potential defense strategies varied across niches in healthy plants, with significant enrichments of functional genes related to biofilm formation in the rhizoplane and antibiotic biosynthesis in the endosphere. Conclusion Our study revealed the varied community composition and assembly mechanism of microbial communities between healthy and diseased tobacco plants along the soil-root continuum, providing new insights into niche-specific defense mechanisms against pathogen invasions. These findings may underscore the potential utilization of different functional prebiotics to enhance plants’ ability to fend off pathogens.

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“…The leaf samples were grounded using pestle mortar after adding liquid nitrogen, added 2 ml of phosphate buffer (pH 6.0), and centrifuged for 15 minutes at 15000 rpm with the help of refrigerator centrifuge separately for each of the tested enzymes viz., peroxidase (POD) and polyphenol oxidase (PPO). In the case of phenylalanine ammonia lyase (PAL), 3 ml of 100 mM borate buffer having the pH 8.7 was added to the 2 g leaf sample after grinding, spun at 10000 rpm, 4ºC for 25 mins in refrigerated centrifuge, and the supernatant was collected for analysis of PAL (Savani et al 2020). The leaf samples of the rapeseed plants were collected at 24 h intervals up to 96 h after releasing the aphids and analyzed to gure out the change in activity of defensive enzymes.…”

Section: Plant Defensive Enzyme Analysis Preparation Of Enzyme Extractsmentioning

confidence: 99%

“…Then 0.5 ml of 3% H 2 O 2 was added, and the optical density (OD) was measured at 436 nm between 30 s and 150 s. A reaction mixture consists of 3.0 ml of 0.05 M pyrogallol and 0.5 ml of 3% H 2 O 2 was served as a blank. The unit activity/ g fresh weight per minute was used to express the POD activity (Savani et al 2020).…”

Section: Determination Of Pod Activitymentioning

confidence: 99%

Effect of foliar application of Silicic acid on biological parameters of Lipaphis erysimi and activity of plant defensive enzymes in rapeseed

Deka,

Ajith

et al. 2024

Preprint

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Silicic acid (SA) is an important source of silicon (Si) that induces resistance in plants against insect pests. The present investigation aimed to investigate the impact of foliar spray of SA on the biological parameters of aphid, Lipaphis erysimi and the activity of defensive enzymes in rapeseed. The results demonstrated applying 0.4% SA significantly reduced the nymphal period, adult longevity and fecundity of L. erysimi compared to the control. In the 0.4% SA- treated rapeseed, the nymphal period, adult longevity and fecundity of L. erysimi were 7.00 days, 7.96 days and 23.52 nymphs/ female, respectively, while in the control, these were 7.92 days, 8.80 days and 26.04 nymphs/ female, respectively. The present investigation revealed that there were no significant changes in activity of defense related enzymes such as peroxidase (POD), polyphenol oxidase (PPO) and phenylalanine ammonia- lyase (PAL) in 0.4% SA- treated rapeseed without aphid infestation. However, a significant increase in the activity of these enzymes was observed in rapeseed amended with 0.4% SA that were subsequently infested with aphids. The application of SA significantly increased the Si content in rapeseed. Furthermore, the study established a significant negative correlation between Si content and biological parameters of L. erysimi.

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Endophyte mediated activation of defense enzymes in banana plants pre-immunized with covert endophytes

Cited by 18 publications

References 21 publications

Impacts of the Biocontrol Strain Pseudomonas simiae PICF7 on the Banana Holobiont: Alteration of Root Microbial Co-occurrence Networks and Effect on Host Defense Responses

Impacts of the Biocontrol Strain Pseudomonas simiae PICF7 on the Banana Holobiont: Alteration of Root Microbial Co-occurrence Networks and Effect on Host Defense Responses

Bacterial wilt affects the structure and assembly of microbial communities along the soil-root continuum

Effect of foliar application of Silicic acid on biological parameters of Lipaphis erysimi and activity of plant defensive enzymes in rapeseed

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