Endophytic bacteria fromPinellia ternata, a new source of purine alkaloids and bacterial manure

Abstract

Context: Pinellia ternata (Thunb.) Berit., a perennial herb belonging to Araceae, is one of the few medicinal plants to produce purine alkaloids. It is speculated that endophytic bacteria from P. ternata may produce guanosine or inosine. However, there is no report about endophytic bacteria in P. ternata. Objective: In this study, endophytic bacteria were isolated from P. ternata and examined for the first time. This study finds a novel way to increase the yield of P. ternata herb, and to provide some new alkaloid producers. Materials and methods: Plant material includes leaves, tubers, and roots of cultivated and wild P. ternata. The dilutions were smeared onto beef extract-peptone medium and cultured at 28 C in darkness for 48-72 h. Co-culture treatments were prepared by inoculating 100 mL liquid 1/2 MS medium with bacterial culture broth at concentrations of 0 (control), 0.5%, and 1.5% (v/v). Results: Of the 34 endophytic bacterial colonies isolated from P. ternata leaves, roots, and tubers, five strains were able to produce purine alkaloids. Results from 16s rDNA sequence analysis indicated that the bacteria belonged to Bacillus cereus, Aranicola proteolyticus, Serratia liquefaciens, Bacillus thuringiensis, and Bacillus licheniformis. Co-culture with living Serratia liquefaciens cells increased PLB growth by 58-71%. Co-culture with living Bacillus licheniformis cells increased PLB growth by 4-11%. Conclusion: This study provides a novel way for improving the yield of P. ternata herb, and for the production of purine alkaloids by the fermentation industry.

Beneficial Relationships Between Endophytic Bacteria and Medicinal Plants

“…Bacteria applied in biological fertilizer can improve plant nutrition and provide an environmentally sustainable means of improving the growth and yield of plants (Vaishnav et al, 2018). Furthermore, the ability of endophytic bacteria to stimulate both production and accumulation of secondary metabolites lends itself to valuable practical applications (Liu et al, 2015). Inoculation with one or several endophytic bacteria has enormous potentials for enhancing bio-active compound production by medicinal plants (Zhou et al, 2015).…”

Antimicrobial Activity Against Phytopathogens and Inhibitory Activity on Solanine in Potatoes of the Endophytic Bacteria Isolated From Potato Tubers

“…Endophytic bacteria of plants may produce a range of different metabolites in vitro and within host plant tissue, which are rich sources of secondary metabolites with biological activity ( Brader et al, 2014 ; Miliute et al, 2015 ). The bioactive secondary metabolites such as alkaloids, terpenoids, phenols, antibiotics, and anti-cancerous compounds produced by endophytic bacteria have been applied in agricultural, industrial, and medical fields ( Christina et al, 2013 ; Gao et al, 2015 ; Liu et al, 2015 ; Yamada et al, 2015 ; Hashem et al, 2016 ). Bacillus stratosphericus LW-03 isolated from the bulbs of Lilium wardii exhibited multiple plant growth-promoting traits, including the production of indole-3-acetic acid (IAA), organic acids, and siderophores ( Khan et al, 2020 ).…”

“…The fermentation broth of Aranicola proteolyticus , Bacillus licheniformis , Bacillus thuringiensis , Bacillus cereus , and Serratia liquefaciens isolated from Pinellia ternata (Thunb.) berit contained alkaloids that were similar to those of the host plants ( Liu et al, 2015 ). In the recent 3 years, at least 128 new endophytic bacteria species of plants were published, including four new genera: Roseitalea , Allobranchiibius , Mangrovicella , and Oceaniradius ( Ai et al, 2017 ; Hyeon et al, 2017 ; Li et al, 2018 ; Jeong et al, 2019 ).…”

Synergistic effects of natural products and commercial antibiotics—A mini–review 2010–2015

“…doi: medRxiv preprint Cont. Table 2: Summary of purified natural products screened for antibiotic modulatory effects of commercially available drugs against nosocomial bacteria, that yielded synergistic effects 35,55,63,69,75,80,104,[104][105][106][107][108][109][110] . Table 3: Summary of plants screened for antimicrobial modulatory effects of commercially available drugs, that yielded synergistic effects against fungi [111][112][113][114] .…”