Endophytic Bacteria from the Sahara Desert Protect Tomato Plants Against Botrytis cinerea Under Different Experimental Conditions

Oukala, Nadira; Pastor-Fernández, Julia; Sanmartín, Neus; Aissat, Kamel; Pastor, Victoria

doi:10.1007/s00284-021-02483-z

Cited by 7 publications

(4 citation statements)

References 47 publications

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“…Most of the bacterial endophytes were positive for the production of all the bioactive compounds considered. The direct release of antimicrobial compounds like antibiotics, siderophores, hydrolytic enzymes, other secondary metabolites, the competition between endophytes and pathogens for space and their capacity to modulate plant defence are some of the ways in which endophytes protect the plant against pests and pathogens (Santoyo et al, 2016;Oukala et al, 2021). Similar results were observed in previous studies where ten bacterial endophytes were selected and identified.…”

Section: Enumeration Of Microflora From the Soil And The Seedlings In...supporting

confidence: 77%

The biocontrol efficacy endophytic bacteria against fungal plant pathogens Cercospora punicae and Ceratocystis fimbriata

Thankam,

Manuel

2024

Preprint

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With the constant raise in the world population, the demand for food supply has increased. Even though the use of agrochemicals helps in controlling pests and pathogens and increasing yield, it causes major environmental pollution. Hence, biocontrol which involves the use of living organisms or their metabolic by-products to control pests and pathogens, is important. Endophytes are a major biocontrol’s used and their association with other symbiotic bacteria helps in improving nutrient acquisition and resistance. In the present study the biocontrol efficacy of six endophytic bacteria (bacterial consortium) were assessed against two fungal pathogens (Cercospora punicae and Ceratocystis fimbriata). The seedlings were inoculated with bacterial consortium (Test). The untreated seedlings were labelled as Control. They were challenge inoculated with fungal pathogens and the plant growth parameters were recorded for 45 days. Percentage of infection, biological control efficacy, and disease incidence were calculated. The successful transmission of the endophytes was assessed through isolation of the endophytes from the soil and the seedlings and imaging the plant tissues using confocal microscope. The ability of endophytes to release certain bioactive compounds like chitinase, siderophores, indole acetic acid, hydrogen cyanide, and 2,4- Diacetyl phloroglucinol was also analysed. The seedlings in Test showed better growth and resistance against fungal pathogens when compared to Control. The biocontrol efficacy was 87.45 ± 2.55 and 88.47 ± 2.79% in Test respectively. Confocal imaging showed successful transmission of endophytes into the plant tissue. The endophytes were able to release bioactive compounds, thus increasing the plant’s ability to resist fungal pathogens and grow.

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Section: Enumeration Of Microflora From the Soil And The Seedlings In...supporting

confidence: 77%

The biocontrol efficacy endophytic bacteria against fungal plant pathogens Cercospora punicae and Ceratocystis fimbriata

Thankam,

Manuel

2024

Preprint

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“…Several studies have shown that plants may recognize and select their specific microbiota to stablish intimate associations [8,11,12]. Trade-off for such interaction may be based in the capacity of the plant host to provide niches for the microbial partner, and endophytes may produce helpful metabolites and signals [13,14], which can increase nutrient uptake and promote plant growth [15], induce resistance against pathogens [16][17][18][19] and insect herbivores [20], and increase plant tolerance to salinity [21], low temperature [22], heavy metals [23], contaminated chemicals [24], and other abiotic factors. Recent publications have added an additional factor and propose to revisit the disease triangle to consider all the effects produced by the endophytes, in terms of disease suppression from an ecological point of view, going beyond the classic studies [25].…”

Section: Introductionmentioning

confidence: 99%

“…The abilities of bacterial endophytes to protect plants against pathogens occur through direct mechanisms, such as the release of antimicrobial compounds such as siderophores, antibiotics, hydrolytic enzymes, and other secondary metabolites, and indirect mechanisms which are related to the competition with pathogens for space and nutrients and their ability to modulate plant defense responses [2,19,29].…”

Section: Introductionmentioning

confidence: 99%

Bacterial Endophytes: The Hidden Actor in Plant Immune Responses against Biotic Stress

Oukala

Aissat

Pastor

2021

Plants

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Bacterial endophytes constitute an essential part of the plant microbiome and are described to promote plant health by different mechanisms. The close interaction with the host leads to important changes in the physiology of the plant. Although beneficial bacteria use the same entrance strategies as bacterial pathogens to colonize and enter the inner plant tissues, the host develops strategies to select and allow the entrance to specific genera of bacteria. In addition, endophytes may modify their own genome to adapt or avoid the defense machinery of the host. The present review gives an overview about bacterial endophytes inhabiting the phytosphere, their diversity, and the interaction with the host. Direct and indirect defenses promoted by the plant–endophyte symbiont exert an important role in controlling plant defenses against different stresses, and here, more specifically, is discussed the role against biotic stress. Defenses that should be considered are the emission of volatiles or antibiotic compounds, but also the induction of basal defenses and boosting plant immunity by priming defenses. The primed defenses may encompass pathogenesis-related protein genes (PR family), antioxidant enzymes, or changes in the secondary metabolism.

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“…The growth ratio was obtained by dividing the colony diameter on CYA with the bacteria over the colony diameter on CYA alone. Additionally, when inhibition was observed, the potential antifungal activity of volatile organic compounds (VOCs) was evaluated according to (Oukala et al, 2021). In detail, the two room-plate method was used against the fungal pathogens reported above using at least three replicates for each bacterial isolate.…”

Section: In Vitro Antagonism Screening Against Grape Fungal Pathogens...mentioning

confidence: 99%

Back to the holobiont: ecophysiological and systemic responses of rooted-cuttings inoculated with a synthetic community

Sandrini,

Chitarra,

Pagliarani

et al. 2023

Preprint

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Despite microbe-based products for grapevine protection and growth improvement are already available, a few of them contain microbes directly isolated from vine tissues. For this reason, a collection of endophytic bacterial isolates obtained directly from grapevine woody tissues has been used for producing anad-hocinoculum. The selected bacterial isolates were tested in biocontrol assays against some of the main grapevine pathogens (e.g.,Botrytis cinerea,Guignardia bidwellii,Neofusicoccum parvum) and the best performing ones were screened for plant growth promoting (PGP)-traits (e.g., phosphorous solubilization, indole-acetic acid and siderophore production). Before being planted, rooted cuttings were inoculated with two different synthetic communities: the first one was anad-hocdeveloped microbial community (SynCom), whereas the second was a commercial consortium formed by arbuscular mycorrhizal fungi (AMF) and a rhizosphere bacterial strain (AMF+B). Physiological parameters were monitored to evaluate effects on plant performances, and samples for biochemical and molecular analyses were collected. Integration of physiological data with metabolite profiling and transcriptome sequencing highlighted that the SynCom treatment shaped the plant growth-defence trade-off, by regulating photosynthesis and diverting energy sources towards the activation of defence metabolic pathways. On the other hand, the AMF+B treatment led to a more balanced growth-defence trade-off, though a mild activation of defence mechanisms was also observed in these plants. Our findings suggest that an experimental approach considering both the features of associated microbes and their impacts on plant growth and defence could shed light on the “dark-side effects” of SynCom application, thereby enabling their exploitation with a refined awareness.

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Endophytic Bacteria from the Sahara Desert Protect Tomato Plants Against Botrytis cinerea Under Different Experimental Conditions

Cited by 7 publications

References 47 publications

The biocontrol efficacy endophytic bacteria against fungal plant pathogens Cercospora punicae and Ceratocystis fimbriata

The biocontrol efficacy endophytic bacteria against fungal plant pathogens Cercospora punicae and Ceratocystis fimbriata

Bacterial Endophytes: The Hidden Actor in Plant Immune Responses against Biotic Stress

Back to the holobiont: ecophysiological and systemic responses of rooted-cuttings inoculated with a synthetic community

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