Endophytic Bacteria Isolated from Citrus Plants for Biological Control of Citrus Canker in Lime Plants

Daungfu, Orawan; Youpensuk, Somchit; Lumyong, Saisamorn

doi:10.21315/tlsr2019.30.1.5

Cited by 49 publications

(27 citation statements)

References 20 publications

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“…B. subtilis AF 1 was used as biological agent to control soft rot in lemons [ 42 ]. B. subtilis LE24, B. amyloliquefaciens LE109, and B. tequilensis PO80 isolated from citrus plants can control the occurrence of citrus canker in lime [ 43 ].…”

Section: Discussionmentioning

confidence: 99%

Antibacterial Potential of Bacillus amyloliquefaciens GJ1 against Citrus Huanglongbing

Nan

Zhang

Jiang

2021

Plants

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Citrus huanglongbing (HLB) is a destructive disease caused by Candidatus Liberibacter species and is a serious global concern for the citrus industry. To date, there is no established strategy for control of this disease. Previously, Bacillus amyloliquefaciens GJ1 was screened as the biocontrol agent against HLB. In this study, two-year-old citrus infected by Ca. L. asiaticus were treated with B. amyloliquefaciens GJ1 solution via root irrigation. In these plants, after seven irrigation treatments, the results indicated that the photosynthetic parameters, chlorophyll content, resistance-associated enzyme content and the expression of defense-related genes were significantly higher than for the plants treated with the same volume water. The content of starch and soluble sugar were significantly lower, compared to the control treatment. The parallel reaction monitoring (PRM) results revealed that treatment with B. amyloliquefaciens GJ1 solution, the expression levels of 3 proteins with photosynthetic function were upregulated in citrus leaves. The accumulation of reactive oxygen species (ROS) in citrus leaves treated with B. amyloliquefaciens GJ1 flag22 was significantly higher than untreated plants and induced the defense-related gene expression in citrus. Finally, surfactin was identified from the fermentation broth of B. amyloliquefaciens GJ1 by high-performance liquid chromatography. These results indicate that B. amyloliquefaciens GJ1 may improve the immunity of citrus by increasing the photosynthesis and enhancing the expression of the resistance-related genes.

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Section: Discussionmentioning

confidence: 99%

Antibacterial Potential of Bacillus amyloliquefaciens GJ1 against Citrus Huanglongbing

Nan

Zhang

Jiang

2021

Plants

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“…A number of Bacillus spp. isolated from citrus rhizosphere and leaves have also been proposed as a biocontrol agent for citrus canker, as they antagonized X. citri growth in vitro and in field conditions (Das et al 2013;Huang et al 2012;Daungfu et al 2019;Rabbee et al 2019). Of note, different species of Pseudomonas, Bacillus and Citrobacter isolated from sweet orange phylloplane inhibited X. citri growth and canker formation by degrading the X. citri quorum sensing molecule DSF (Caicedo et al 2016).…”

Section: Biological Controlmentioning

confidence: 99%

Xanthomonas citri subsp. citri: host interaction and control strategies

Martins

Andrade

Benedetti

et al. 2020

Trop. plant pathol.

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Citrus is one of the most ancient fruit crops cultivated in the world. For many countries, citrus production is an important source of revenues. However, despite the richness of citrus production worldwide, fruit yields are constantly threatened by diseases that cause serious economic and social impacts to growers and consumers. One such example is citrus canker, a disease that affects all commercial citrus varieties and for which there is no cure. Currently, control measures for citrus canker are restricted to the application of copper-based compounds and the elimination of infected trees to prevent pathogen spreading in the field. However, new alternatives for the control of this disease are being developed, spanning through transgenic plants to innovative chemicals and biological control. New genome editing approaches and a repertoire of plant defense-related genes have been exploited to produce citrus lineages more tolerant to X. citri. In addition, a series of new molecules have been tested to not only inhibit X. citri growth but to also boost the plant immune system to suppress disease progression. In this review, we provide the state of art of all citrus canker control measures in use today, highlighting their utility and drawbacks and commenting on novel strategies to better control this important citrus disease.

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“…B. amyloliquefaciens strains were reported to antagonize X. citri sbsp. citri [ 6 ], X. axonopodis pv. dieffenbachiae [ 7 ], and X. campestris pv.…”

Section: Introductionmentioning

confidence: 99%

Killing Effect of Bacillus velezensis FZB42 on a Xanthomonas campestris pv. Campestris (Xcc) Strain Newly Isolated from Cabbage Brassica oleracea Convar. Capitata (L.): A Metabolomic Study

et al. 2021

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The potential use of Bacillus velezensis FZB42 for biological control of various phytopathogens has been documented over the past few years, but its antagonistic interactions with xanthomonads has not been studied in detail. Novel aspects in this study consist of close observation of the death of Xanthomonas campestris pv. campestris cells in a co-culture with B. velezensis FZB42, and quantification of lipopeptides and a siderophore, bacillibactin, involved in the killing process. A new robust Xcc-SU isolate tolerating high concentrations of ferric ions was used. In a co-culture with the antagonist, the population of Xcc-SU was entirely destroyed within 24–48 h, depending on the number of antagonist cells used for inoculation. No inhibitory effect of Xcc-SU on B. velezensis was observed. Bacillibactin and lipopeptides (surfactin, fengycin, and bacillomycin) were present in the co-culture and the monoculture of B. velezensis. Except for bacillibactin, the maximum contents of lipopeptides were higher in the antagonist monoculture compared with the co-culture. Scanning electron microscopy showed that the death of Xcc-SU bacteria in co-culture was caused by cell lysis, leading to an enhanced occurrence of distorted cells and cell ghosts. Analysis by mass spectrometry showed four significant compounds, bacillibactin, surfactin, fengycin, and bacillomycin D amongst a total of 24 different forms detected in the co-culture supernatant: Different forms of surfactin and fengycin with variations in their side-chain length were also detected. These results demonstrate the ability of B. velezensis FZB42 to act as a potent antagonistic strain against Xcc.

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Endophytic Bacteria Isolated from Citrus Plants for Biological Control of Citrus Canker in Lime Plants

Cited by 49 publications

References 20 publications

Antibacterial Potential of Bacillus amyloliquefaciens GJ1 against Citrus Huanglongbing

Antibacterial Potential of Bacillus amyloliquefaciens GJ1 against Citrus Huanglongbing

Xanthomonas citri subsp. citri: host interaction and control strategies

Killing Effect of Bacillus velezensis FZB42 on a Xanthomonas campestris pv. Campestris (Xcc) Strain Newly Isolated from Cabbage Brassica oleracea Convar. Capitata (L.): A Metabolomic Study

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