Endophytic bacterial diversity in grapevine (Vitis vinifera L.) leaves described by 16S rRNA gene sequence analysis and length heterogeneity-PCR

Bulgari, Daniela; Casati, P.; Brusetti, Lorenzo; Quaglino, F.; Brasca, Milena; Daffonchio, Daniele; Bianco, P.A.

doi:10.1007/s12275-009-0082-1

Cited by 81 publications

(52 citation statements)

References 54 publications

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“…Pantoea is an endophytic resident of different plants, such as rice, soybean, sweet potato, grapevine, and maize (Elvira-Recuenco and van Vuurde, 2000; Bulgari et al, 2009). Interestingly, the strain TR-5 of Pantoea ananatis isolated from maize kernels inhibited in vitro the growth of the fungus Lecanicillium aphanocladii.…”

Section: Discussionmentioning

confidence: 99%

Diversity of bacterial endophytes in roots of Mexican husk tomato plants (Physalis ixocarpa) and their detection in the rhizosphere

et al. 2010

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ABSTRACT. Endophytic bacterial diversity was estimated in Mexican husk tomato plant roots by amplified rDNA restriction analysis and sequence homology comparison of the 16S rDNA genes. Sixteen operational taxonomic units from the 16S rDNA root library were identified based on sequence analysis, including the classes Gammaproteobacteria, Betaproteobacteria, Actinobacteria, and Bacilli. The predominant genera were Stenotrophomonas (21.9%), Microbacterium (17.1%), Burkholderia (14.3%), Bacillus (14.3%), and Pseudomonas (10.5%). In a 16S rDNA gene library of the same plant species' rhizosphere, only common soil bacteria, including Stenotrophomonas, Burkholderia, Bacillus, and Bacterial endophytic diversity in Physalis ixocarpaPseudomonas, were detected. We suggest that the endophytic bacterial diversity within the roots of Mexican husk tomato plants is a subset of the rhizosphere bacterial population, dominated by a few genera.

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Section: Discussionmentioning

confidence: 99%

Diversity of bacterial endophytes in roots of Mexican husk tomato plants (Physalis ixocarpa) and their detection in the rhizosphere

et al. 2010

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“…Recently, endophytic communities associated with healthy and phytoplasmainfected plants have been characterised (Bulgari et al, 2009;Martini et al, 2009) to find possible biocontrol agents. Martini et al (2009) Musetti et al (2011) showed that periwinkle plants, Catharanthus roseus L. G. Don, grafted with "Ca.…”

Section: Introductionmentioning

confidence: 99%

Microbial diversity in leaves, trunk and rhizosphere of coconut palms (Cocos nucifera L.) associated with the coconut lethal yellowing phytoplasma in Grand-Lahou, Cte dIvoire

Morales-Lizcano¹,

Hasan²,

To³

et al. 2017

Afr. J. Biotechnol.

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The diversity of culturable bacterial and fungal communities was assessed from leaves, trunk and rhizosphere of coconut palms infected and non-infected by the Côte d'Ivoire lethal yellowing (CILY) phytoplasma. Bacterial and fungal microbes were isolated from leaves, trunk and rhizosphere samples collected from two villages of Grand-Lahou, Braffedon and Yaokro, by using a high-throughput 'dilution-to-extinction' cultivation method coupled with PCR and sequencing with primers that amplified both the 16S ribosomal RNA and intergenic transcribed spacer (ITS) genes. The relative abundance was higher for Bacillus and Candida in Braffedon, and Burkholderia and Neodeightonia in Yaokro. Commonly genera identified from rhizosphere included Bacillus, Burkholderia, Pseudomonas, Streptomyces, Cryptococcus, Penicillium, Purpureocillium and Trichoderma. The most abundant endophytes identified were Pantoea, Candida, Cryptococcus, Bacillus, Pseudomonas, Penicillium, Aspergillus and Rhodotorula. Genera limited to symptomless palms included Arthrininum, Chaetomium, Phialemonium, Fusarium, Klebsiella and Candida. Results indicate that the CILY phytoplasma may be a factor determining the level of diversity of a microbial community in a given location. Our research provides the basis to investigate the possible effect of endophytic and rhizosphere microbes against the CILY phytoplasma to further effectively improve the management of CILY in Grand-Lahou.

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“…However, the relation between communities of endophytic fungi and host plants is still poorly studied, and as of yet, is far from being fully understood. In grapevines, recent studies have shed some light upon the bacterial endophytic communities (13,14,20,47,72), while investigations on fungal communities have been rare and often limited to culture-dependent methods (20,30,43,71). Furthermore, research has mainly focused on subsoil plant-microbe associations (36,65).…”

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Fungal Endophytic Communities in Grapevines (Vitis vinifera L.) Respond to Crop Management

Pancher

Ceol

Corneo

et al. 2012

Appl Environ Microbiol

165

127

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ABSTRACTWe studied the distribution of fungal endophytes of grapevine (Vitis viniferaL.) plants in a subalpine area of northern Italy, where viticulture is of high economic relevance. We adopted both cultivation-based and cultivation-independent approaches to address how various anthropic and nonanthropic factors shape microbial communities. Grapevine stems were harvested from several locations considering organic and integrated pest management (IPM) and from the cultivars Merlot and Chardonnay. Cultivable fungi were isolated and identified by internal-transcribed-spacer sequence analysis, using a novel colony-PCR method, to amplify DNA from fungal specimens. The composition of fungal communities was assessed using a cultivation-independent approach, automated ribosomal intergenic spacer analysis (ARISA). Multivariate statistical analysis of both culture-dependent and culture-independent data sets was convergent and indicated that fungal endophytic communities in grapevines from organically managed farms were different from those from farms utilizing IPM. Fungal communities in plants of cv. Merlot and cv. Chardonnay overlapped when analyzed using culture-dependent approaches but could be partially resolved using ARISA fingerprinting.

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Endophytic bacterial diversity in grapevine (Vitis vinifera L.) leaves described by 16S rRNA gene sequence analysis and length heterogeneity-PCR

Cited by 81 publications

References 54 publications

Diversity of bacterial endophytes in roots of Mexican husk tomato plants (Physalis ixocarpa) and their detection in the rhizosphere

Diversity of bacterial endophytes in roots of Mexican husk tomato plants (Physalis ixocarpa) and their detection in the rhizosphere

Microbial diversity in leaves, trunk and rhizosphere of coconut palms (Cocos nucifera L.) associated with the coconut lethal yellowing phytoplasma in Grand-Lahou, Cte dIvoire

Fungal Endophytic Communities in Grapevines (Vitis vinifera L.) Respond to Crop Management

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