Endophytic Bacterial Microbiome Diversity in Early Developmental Stage Plant Tissues of Wheat Varieties

Žiarovská, Jana; Medo, Juraj; Kyseľ, Matúš; Zamiešková, Lucia; Kačániová, Miroslava

doi:10.3390/plants9020266

Cited by 26 publications

(18 citation statements)

References 41 publications

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“…Furthermore, there is some information in the literature that the phenomena of, for example, the accumulation of starch and the loss of water during the seed maturation process seem to favor endophytes that are tolerant to high osmotic pressure [ 4 , 32 ]. Consequently, the endospore formation can also be an important feature for seed colonizers, as it protects them from changes occurring inside the seed [ 32 , 33 ]. The heatmaps presented in Figure 2 and Figure 4 included: (1) bacteria that were present in at least 10 reads and (2) bacteria that were present in at least three samples (or two samples for the in vitro plants).…”

Section: Discussionmentioning

confidence: 99%

New Insight into the Composition of Wheat Seed Microbiota

Kuźniar

Włodarczyk

Grządziel

et al. 2020

IJMS

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Endophytes are associated with host plants throughout their life history from seed germination to fruit development. One of the most important plant organs colonized by endophytic microbiota is the seed. The aim of this study was to determine the structure of the seed core microbiome inhabiting the endosperms and embryos of eight wheat cultivars with the use of a culture-independent technique. The seeds of Triticum aestivum L. cv. Hondia, Wilejka, STH, Opcja, Tybalt, Euforia and Triticum spelta L. cv. Rokosz and Schwabencorn (producer: Plant Breeding Strzelce Sp. z o.o. Group IHAR) were studied. Rokosz and Hondia were cultured in vitro and in vivo to identify obligatory bacterial endophytes. A restrictive analysis of reads originating from the in vitro plants has demonstrated that the bacterial genera Paenibacillus and Propionibacterium inhabiting Rokosz and Hondia plants have a status of obligatory microorganisms. Greater biodiversity of seed-borne endophytes was found in the seed endosperms than in the embryos. The multiple comparison analysis of the OTU abundance indicated that the seed part significantly influenced the relative abundance. The seed-born microbiome is not statistically significantly dependent on the wheat cultivars; however, it cannot be claimed that every wheat seed is the same.

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Section: Discussionmentioning

confidence: 99%

New Insight into the Composition of Wheat Seed Microbiota

Kuźniar

Włodarczyk

Grządziel

et al. 2020

IJMS

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“…The endophytic bacterial communities are known to shift with respect to age of plant and environmental factors [6,38]. Most of the earlier studies have focused on analyzing the endophytic bacteria at specific plant growth stages in wheat [1,8,39,40]. The present work emphasizes the study on distribution of nitrogen-fixing endophytic bacteria in wheat crop during different developmental phases.…”

Section: Discussionmentioning

confidence: 99%

Wheat Growth Dependent Succession of Culturable Endophytic Bacteria and Their Plant Growth Promoting Traits

et al. 2021

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Endophytic bacteria present ubiquitously in all plant parts. Their community structure may vary depending on plant tissue and growth condition. This work mainly focused on exploring the diversity of culturable nitrogen-fixing endophytic bacteria in above-ground plant parts of wheat by harvesting it during various growth points (Seed stage, 1st, 2nd, and 3rd month old plants, respectively). Distinct endophytic bacterial colonies were selected on Jensen's agar plate. Based on the 16S rRNA sequencing, 43 putative nitrogen-fixing endophytic bacteria were identified. Most of the isolates were found unique to the plant growth phase except for Pseudomonas sp., Bacillus sp., Paenibacillus sp., Microbacterium sp., Exiguobacterium sp. Further, endophytic bacteria were scrutinized for their plant growth promoting traits. They were found positive for IAA production (100%), P-solubilization (21%), Zn-solubilization (63%), ammonia production (93%), and nifH gene (33%). Extracellular enzyme production was found positive for cellulase (98%), pectinase (98%), and protease (100%). Their endophytic colonization ability was assessed using reactive oxygen species (ROS) induction assay, upon their entry inside the host plant.

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“…PCoA also showed that endophytic communities from C. pilosula parts with drought treatment were distinguishable from those in untreated parts. The endophytic bacteria in wheat under drought stress were definitely different from those in control samples [ 44 ]. Previous studies showed that the increase in endophytic diversity and the variation in composition were beneficial to the formation of complexes and stable networks, which might enhance the endophyte, endophyte–endophyte, and endophyte–plant interaction to implement the potential functions of the microorganism [ 41 ].…”

Section: Discussionmentioning

confidence: 99%

Contents of lobetyolin, syringin, and atractylolide III in Codonopsis pilosula are related to dynamic changes of endophytes under drought stress

et al. 2021

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Background Codonopsis pilosula, an important medicinal plant, can accumulate certain metabolites under moderate drought stress. Endophytes are involved in the metabolite accumulations within medicinal plants. It is still unknown that the endophytes of C. pilosula are associated with the accumulations of metabolites. This study aims to investigate the promoting effect of endophytes on the accumulations of active substances in C. pilosula under drought stress. Methods High–performance liquid chromatography and high–throughput sequencing technology were performed to investigate changes in the contents of secondary metabolite and endophyte abundances of C. pilosula under drought stress, respectively. Spearman’s correlation analysis was further conducted to identify the endophytic biomarkers related to accumulations of pharmacodynamic compounds. Culture-dependent experiments were performed to confirm the functions of endophytes in metabolite accumulations. Results The distribution of pharmacological components and diversity and composition of endophytes showed tissue specificity within C. pilosula. The contents of lobetyolin, syringin, and atractylolide III in C. pilosula under drought stress were increased by 8.47%‒86.47%, 28.78%‒230.98%, and 32.17%‒177.86%, respectively, in comparison with those in untreated groups. The Chao 1 and Shannon indices in different parts of drought–stressed C. pilosula increased compared with those in untreated parts. The composition of endophytic communities in drought treatment parts of C. pilosula was different from that in control parts. A total of 226 microbial taxa were identified as potential biomarkers, of which the abundances of 42 taxa were significantly and positively correlated to the pharmacodynamic contents. Culture-dependent experiments confirmed that the contents of lobetyolin and atractylolide III were increased by the application of Epicoccum thailandicum, Filobasidium magnum, and Paraphoma rhaphiolepidis at the rates of 11.12%‒46.02%, and that the content of syringin was increased by Pseudomonas nitroreducens at the rates of 118.61%‒119.36%. Conclusions Certain endophytes participated in the accumulations of bioactive metabolites, which provided a scientific evidence for the development and application of microorganisms to improve the quality of traditional Chinese medicine.

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Endophytic Bacterial Microbiome Diversity in Early Developmental Stage Plant Tissues of Wheat Varieties

Cited by 26 publications

References 41 publications

New Insight into the Composition of Wheat Seed Microbiota

New Insight into the Composition of Wheat Seed Microbiota

Wheat Growth Dependent Succession of Culturable Endophytic Bacteria and Their Plant Growth Promoting Traits

Contents of lobetyolin, syringin, and atractylolide III in Codonopsis pilosula are related to dynamic changes of endophytes under drought stress

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