Endophytic bacterium Pseudomonas protegens suppresses mycelial growth of Botryosphaeria dothidea and decreases its pathogenicity to postharvest fruits

Huang, Yonghong; Liu, Junping; Li, Jinghui; Shan, Xiaoying; Duan, Yanxin

doi:10.3389/fmicb.2022.1069517

Cited by 7 publications

(7 citation statements)

References 60 publications

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“…and B. velezensis have also demonstrated their efficacy against fungal trunk diseases in other crops, such as grapevine, 37,39,55 as well as in the control of apple and pear ring rot disease caused by B. dothidea. 48,[74][75][76] Isolation of endophytic bacteria from healthy, asymptomatic trees, growing in an infested orchard has been described as a powerful technic to obtain bacterial species to be used as (A) (B) Figure 3. Severity of symptoms (A) length of the lesions (B) on almond plants cv.…”

Section: Discussionmentioning

confidence: 99%

“…velezensis have also demonstrated their efficacy against fungal trunk diseases in other crops, such as grapevine, 37,39,55 as well as in the control of apple and pear ring rot disease caused by B . dothidea 48,74–76 …”

Section: Discussionmentioning

confidence: 99%

“…15 In addition, several bacterial strains from the genus Bacillus, Streptomyces, Pseudomonas and Xenorhabdus have demonstrated, alone or in combination with other BCAs, their antagonism against Botryosphaeriaceae species in several crops. 38,40,[44][45][46][47][48][49][50][51][52] Rhizospheric bacteria have been demonstrated as potential biocontrol agents of the soil-borne pathogen M. phaseolina within the Botryosphaeriaceae family in strawberry crops. 53,54 However, to the best of our knowledge, no control agents based on bacterial strains have been successfully tested against canker diseases caused by Botryosphaeriaceae in almond.…”

Section: Introductionmentioning

confidence: 99%

“…atroviride SC1 has been registered in California as a biological control agent against almond canker pathogens 15 . In addition, several bacterial strains from the genus Bacillus , Streptomyces , Pseudomonas and Xenorhabdus have demonstrated, alone or in combination with other BCAs, their antagonism against Botryosphaeriaceae species in several crops 38,40,44–52 . Rhizospheric bacteria have been demonstrated as potential biocontrol agents of the soil‐borne pathogen M .…”

Section: Introductionmentioning

confidence: 99%

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Biocontrol of almond canker diseases caused by Botryosphaeriaceae fungi

Romero‐Cuadrado,

Picos,

Camacho

et al. 2023

Pest Management Science

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BACKGROUNDBotryosphaeria dieback is a canker disease caused by fungal species of the Botryosphaeriaceae family that threatens almond productivity. The most common control measure to prevent canker development is the application of fungicides which are being phased out by European Union regulations. In the present study, two sets of bacterial strains were evaluated for their antifungal activity against pathogenic Botryosphaeriaceae species through in vitro and in vivo antagonism assays.RESULTSThe rhizospheric bacteria Pseudomonas aeruginosa AC17 and Bacillus velezensis ACH16, as well as the endophytic bacteria Bacillus mobilis Sol 1–2, respectively inhibited 87, 95, and 63% of the mycelial growth of Neofusicoccum parvum, Botryosphaeria dothidea, Diplodia seriata, and Macrophomina phaseolina. Additionally, they significantly reduced the length of lesions caused by N. parvum and B. dothidea in artificially inoculated detached almond twigs. All these bacterial strains produce hydrolytic enzymes that are able to degrade the fungal cell wall. P. aeruginosa AC17 also produces toxic volatile compounds, such as hydrogen cyanide. This strain was the most effective in controlling Botryosphaeria dieback in planta under controlled conditions at a level similar to the biocontrol agent Trichoderma atroviride and standard chemical fungicide treatments.CONCLUSIONPseudomonas aeruginosa AC17 is the best candidate to be considered as a potential biocontrol agent against Botryosphaeriaceae fungi affecting almond. © 2023 Society of Chemical Industry.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Biocontrol of almond canker diseases caused by Botryosphaeriaceae fungi

Romero‐Cuadrado,

Picos,

Camacho

et al. 2023

Pest Management Science

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“…The biocontrol potential of beneficial Pseudomonas spp. has been extensively investigated through antibiosis, competition, and elicitation of induced systemic resistance (ISR) ( Balthazar et al., 2022a ; Huang et al., 2022 ). Notably, Pseudomonas fluorescens ZX had ability to protect postharvest citrus fruits against blue and green molds, caused by Penicillium italicum and Penicillium digitatum , respectively ( Wang et al., 2021 ).…”

Section: Introductionmentioning

confidence: 99%

Biocontrol potential of Pseudomonas protegens ML15 against Botrytis cinerea causing gray mold on postharvest tomato (Solanum lycopersicum var. cerasiforme)

Ajijah,

Fiodor,

Dziurzynski

et al. 2023

Front. Plant Sci.

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Gray mold, caused by Botrytis cinerea is a major cause of post-harvest rot of fresh fruits and vegetables. The utilization of selected microorganisms as biocontrol agents is a promising alternative to effectively control gray mold on tomatoes. The current study was conducted to explore potential biocontrol mechanisms of the Pseudomonas strain to control infections on post-harvest tomatoes. Among the 8 tested bacterial isolates, Pseudomonas protegens ML15 demonstrated antagonistic activity to Botrytis cinerea. Moreover, P. protegens ML15 exhibited the production of siderophores, hydrogen cyanide, ammonia, exopolysaccharides, lipase, biosurfactant, 2,4-diacetylphloroglucinol, and several other antifungal compounds, such as 1-tetradecanol, cyclododecane, 2,4-di-tert-butylphenol, and 2-methyl-1-hexadecanol. A comprehensive genomic analysis of P. protegens ML15 unravels 18 distinct genetic regions with the potential for biosynthesizing secondary metabolites, known for their pivotal role in biocontrol responses against plant pathogens. In vivo, experiments showed that both culture suspension and cell-free supernatant of P. protegens ML15 significantly reduced fungal growth (53.0 ± 0.63%) and mitigated disease development (52.8 ± 1.5%) in cherry tomatoes at four days post-B. cinerea inoculation. During the infection, the application of P. protegens ML15 resulted in the augmentation of total antioxidant, phenolic content, and ascorbic acids content. Thus, our results suggested that P. protegens ML15’s role as a biocontrol agent against B. cinerea-induced postharvest tomato decay achieved through the secretion of antifungal substances, induction of tomato defense responses, and inhibition of mycelial growth of B. cinerea. These findings provide a significant contribution to the ongoing search for alternative, eco-friendly methods of controlling gray mold in fresh products. The utilization of P. protegens ML15 as a biocontrol agent could help to reduce the reliance on chemical fungicides and promote sustainable agriculture practices.

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