Endophytic Fungi: A Cryptic Fountainhead for Biodiversity, Functional Metabolites, Host Stress Tolerance, and Myco-Mediated Nanoparticles (Nps) Synthesis

Nishad, Jay Hind; Singh, Arti; Gautam, Veer Singh; Kumar, Dharmendra; Kumar, Jitendra; Kharwar, Ravindra N.

doi:10.1007/978-3-319-76900-4_18-1

Cited by 4 publications

(4 citation statements)

References 71 publications

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“…We show that high doses of L. acidophilus + L. casei + L. plantarum are especially important for regulating Ascomycota and Capnodiales levels. Because ascomycetes are known to be used in medicine with the antibiotics penicillin and cephalosporin [119], and endophytic sooty mold fungi (Dothideomycetes) can be important for tissue health, environmental adaptation, and stress tolerance [120], this could be a signi cant discovery for cancer treatment. Interestingly, high doses of 3L (H treatment) have been shown to stimulate the levels of many different fungi families, including Aspergilaceae, Microascaceae, Nectriaceae, and Trichosporonaceae.…”

Section: Discussionmentioning

confidence: 99%

3L, three-Lactobacilli on recovering of microbiome and immune-damage by cyclophosphamide chemotherapy —A pilot experiment—

Yue

Zhang

Bian

et al. 2023

Preprint

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Purpose We examined the impact of using a probiotic containing three different Lactobacilli(3L) on the gut microbiome of rats following cyclophosphamide (CTX) treatment. CTX corresponded to chemotherapy which is used for human cancer treatment and known to have adversive effects on the immune system. Methods We conducted our experiment with ten rats in five different experimental groups which included control, CTX treated, and then low, medium, and high probiotic treatment with CTX treatment. Of these ten rats in each group, we sequenced the stool of three of them using both ITS and 16S sequencing. We then went on to examine the taxonomic composition of these samples to determine whether probiotic treatment helped the rat’s microbiome return to similar structure as the control rats. Results We used Illumina MiSeq sequencing to generate sequencing data from microbial genomic DNA libraries, which is useful for testing the effects of 3L on bacteria and fungi. Microbiome analysis, phylogenetic and classification reports, and community data have all backed up the experiments and findings that 3L had a significant positive impact on the microbiome. Furthermore, the effect on specific metabolic pathways aids in deriving the study’s conclusion (use of 3L in chemotherapy) to the mode of action, mechanistically by correcting microbiota composition and enhancing specific gut metabolic functions. Conclusions Through experimental results using an in vivo model, we suggested the role of novel natural probiotics 3L, 3 Lactobacilli in the establishment of a strong and sustainable beneficial healthy gut flora, after CTX chemotherapy. We suggested some new adjuvants to chemotherapy as drugs + lactobacillus treament using the rat CTX model (immunosuppression caused by cyclophosphamide). Furthermore, in numerous studies that reported the use of probiotics involving Lactobacillusin post-chemo or post-surgical procedures, we proposed a new probiotic formulation (L. acidophilus + L. casei + L. plantarum) to be further studied and explored in the prevention of health condition loss by alteration of the general immune system.

show abstract

Section: Discussionmentioning

confidence: 99%

3L, three-Lactobacilli on recovering of microbiome and immune-damage by cyclophosphamide chemotherapy —A pilot experiment—

Yue

Zhang

Bian

et al. 2023

Preprint

View full text Add to dashboard Cite

show abstract

“…Because ascomycetes are known to be used in medicine with the antibiotics penicillin and cephalosporin [119], and endophytic sooty mold fungi (Dothideomycetes) can be important for tissue health, environmental adaptation, and stress tolerance [120], this could be a signi cant discovery for cancer treatment. Interestingly, high doses of 3L (H treatment) have been shown to stimulate the levels of many different fungi families, including Aspergilaceae, Microascaceae, Nectriaceae, and Trichosporonaceae.…”

Section: Discussionmentioning

confidence: 99%

3L, three-Lactobacilli on recovering of microbiome and immune-damage by cyclophosphamide chemotherapy —A pilot experiment in rats—

Yue

Zhang

Bian

et al. 2023

Preprint

View full text Add to dashboard Cite

Purpose We examined the impact of using a probiotic containing three different Lactobacilli (3L) on the gut microbiome of rats following cyclophosphamide (CTX) treatment. CTX corresponded to chemotherapy which is used for human cancer treatment and known to have adversive effects on the immune system. Methods We conducted our experiment with ten rats in five different experimental groups which included control, CTX treated, and then low, medium, and high probiotic treatment with CTX treatment. Of these ten rats in each group, we sequenced the stool of three of them using both ITS and 16S sequencing. We then went on to examine the taxonomic composition of these samples to determine whether probiotic treatment helped the rat’s microbiome return to similar structure as the control rats. Results We used Illumina MiSeq sequencing to generate sequencing data from microbial genomic DNA libraries, which is useful for testing the effects of 3L on bacteria and fungi. Microbiome analysis, phylogenetic and classification reports, and community data have all backed up the experiments and findings that 3L had a significant positive impact on the microbiome. Furthermore, the effect on specific metabolic pathways aids in deriving the study’s conclusion (use of 3L in chemotherapy) to the mode of action, mechanistically by correcting microbiota composition and enhancing specific gut metabolic functions. Conclusions Through experimental results using an in vivo model, we suggested the role of novel natural probiotics 3L, 3 Lactobacilli in the establishment of a strong and sustainable beneficial healthy gut flora, after CTX chemotherapy. We suggested some new adjuvants to chemotherapy as drugs + lactobacillus treament using the rat CTX model (immunosuppression caused by cyclophosphamide). Furthermore, in numerous studies that reported the use of probiotics involving Lactobacillus in post-chemo or post-surgical procedures, we proposed a new probiotic formulation (L. acidophilus + L. casei + L. plantarum) to be further studied and explored in the prevention of health condition loss by alteration of the general immune system.

show abstract

“…We show here that high doses of L. acidophilus + L. casei + L. plantarum are particularly relevant to regulate the levels of Ascomycota and Capnodiales. This may be important discovery for cancer treatment due to the finding that ascomycetes are known to be used in medicine with the antibiotics penicillin and cephalosporin [89], while endophytic sooty mold fungi (Dothideomycetes) can be important for tissue health, environmental adaptation and stress tolerance [90].…”

Section: Discussionmentioning

confidence: 99%

<em>Lactobacillus</em> on Recovering of Immune-Damage by Chemotherapy (Cancer Treatment)

Yue¹,

Zhang²,

Bian³

et al. 2022

Preprint

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We demonstrated the role of natural probiotics 3L, 3 Lactobacilli, in the establishment of a strong and sustainable beneficial healthy gut flora, after chemotherapy through experimental results through in vivo model. Using rat CTX model (immunosuppression induced by cyclophosphamide), we suggested some new adjuvant to chemotherapy as drugs + lactobacillus treament. Further, we proposed a new probiotic formulation (L. acidophilus + L. casei + L. plantarum) to be explored in the prevention of health condition loss by alteration of the general immune system, in numerous studies that reported the use of probiotics involving Lactobacillus in the post-chemo or post-surgical procedures. Here, in our study, Illumina MiSeq sequencing was used to generate sequencing data from microbial genomic DNA libraries, which is appreciable to check for the effects of 3L on bacteria. Microbiome analysis, phylogenetic and classification reports, community data have supported the experiments and the results where 3L had strong beneficial effects on the microbiome. Further, the influence on specific metabolic pathways are assisted in deriving the conclusion of the study (use of 3L for cancer therapy) to the mode of action, mechanistically by correcting microbiota composition and enhancing specific gut metabolic functions.

show abstract

Endophytic Fungi: A Cryptic Fountainhead for Biodiversity, Functional Metabolites, Host Stress Tolerance, and Myco-Mediated Nanoparticles (Nps) Synthesis

Cited by 4 publications

References 71 publications

3L, three-Lactobacilli on recovering of microbiome and immune-damage by cyclophosphamide chemotherapy —A pilot experiment—

3L, three-Lactobacilli on recovering of microbiome and immune-damage by cyclophosphamide chemotherapy —A pilot experiment—

3L, three-Lactobacilli on recovering of microbiome and immune-damage by cyclophosphamide chemotherapy —A pilot experiment in rats—

<em>Lactobacillus</em> on Recovering of Immune-Damage by Chemotherapy (Cancer Treatment)

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