Endophytic Fungi as a Source of Biofuel Precursors

Santos-Fo, Florisvaldo C.; Fill, Taícia Pacheco; Nakamura, Joanita; Monteiro, Marcos Roberto; Rodrigues-Fo, Edson

doi:10.4014/jmb.1010.10052

Cited by 16 publications

(2 citation statements)

References 32 publications

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“…In this work, comparative genome analysis of nonoleaginous and oleaginous micro-organisms was performed to investigate candidate orthologous genes and enzymes related to oleaginicity. The oleaginous strains studied were selected based on their genome availability and oleagenicity features, and included Aspergillus oryzae (Kaur & Worgan, 1982;Lin et al, 2010;Meng et al, 2009;Santos-Fo et al, 2011), M. circinelloides (Vicente et al 2010), Rhizopus oryzae (Ratledge, 1986;Suzuki, 1991) and Y. lipolytica (Beopoulos et al, 2009), which have been shown to be potential lipid producers (Ageitos et al, 2011;Ratledge, 2004). We also emphasized the metabolism of acetyl-CoA, which is the key two-carbon metabolite in several metabolic processes, particularly in terms of precursor supply for fatty acid synthesis in oleaginous strains.…”

Section: Introductionmentioning

confidence: 99%

Alternative routes of acetyl-CoA synthesis identified by comparative genomic analysis: involvement in the lipid production of oleaginous yeast and fungi

et al. 2012

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For a bio-based economy, microbial lipids offer a potential solution as alternative feedstocks in the oleochemical industry. The existing genome data for the promising strains, oleaginous yeasts and fungi, allowed us to investigate candidate orthologous sequences that participate in their oleaginicity. Comparative genome analysis of the non-oleaginous (Saccharomyces cerevisiae, Candida albicans and Ashbya gossypii ) and oleaginous strains (Yarrowia lipolytica, Rhizopus oryzae, Aspergillus oryzae and Mucor circinelloides) showed that 209 orthologous protein sequences of the oleaginous microbes were distributed over several processes of the cells. Based on the 41 sequences categorized by metabolism, putative routes potentially involved in the generation of precursors for fatty acid and lipid synthesis, particularly acetyl-CoA, were then identified that were not present in the non-oleaginous strains. We found a set of the orthologous oleaginous proteins that was responsible for the biosynthesis of this key two-carbon metabolite through citrate catabolism, fatty acid b-oxidation, leucine metabolism and lysine degradation. Our findings suggest a relationship between carbohydrate, lipid and amino acid metabolism in the biosynthesis of acetyl-CoA, which contributes to the lipid production of oleaginous microbes. INTRODUCTIONLipids are dynamically bioactive molecules that contribute to the regulation of several complex systems of living cells. Besides the medical perspective, the remarkable growth of the lipid field is undoubtedly driven by the demand for feedstock for the oleochemical industry. In addition to the production of n-3 and n-6 polyunsaturated fatty acids that are beneficial to human health, extensive attention is being directed to biodiesel production from micro-organisms to replace non-sustainable petroleum (Liu & Zhao, 2007; Vicente et al., 2010). Certain strains, such as Rhodosporidiun toruloides, Lipomyces starkeyi, Yarrowia lipolytica and Mucor circinelloides, are known to accumulate substantial amounts of lipids, accounting for more than 20 % of their biomass, and are thus called oleaginous strains (Ageitos et al., 2011;Beopoulos et al., 2009;Meng et al. 2009;Ratledge, 2004). Due to their short cultivation time, their high level of intracellular lipids that are predominantly triacylglycerol (TAG) and their utilization of various substrates, oleaginous yeasts and fungi have become important model systems for alternatives to traditional sources of lipids derived from fossil, animal and plant origins (Beopoulos et al., 2009;Ratledge, 2004). Therefore, an understanding of lipid physiology is required to improve the efficient production of lipids of commercial interest. An integrated approach has been implemented using recent developments for studying the lipid metabolism of these promising micro-organisms. It has been reported that the lipid production of these oleaginous species is enhanced by controlling cultivation or nutritional conditions (Certik et al., 1999;Ruenwai et al., 2010). Based on the bioc...

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Section: Introductionmentioning

confidence: 99%

Alternative routes of acetyl-CoA synthesis identified by comparative genomic analysis: involvement in the lipid production of oleaginous yeast and fungi

et al. 2012

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“…Another important biotechnological application from P. brasilianum was studied by Santos-Fo et al [ 72 ]. The authors investigated the potential production of lipid biodiesel precursors from liquid culture produced by P. brasilianum and other endophytic fungi.…”

Section: Biotechnological Potential Of P Brasilianummentioning

confidence: 99%

Insights into Penicillium brasilianum Secondary Metabolism and Its Biotechnological Potential

et al. 2017

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Over the past few years Penicillium brasilianum has been isolated from many different environmental sources as soil isolates, plant endophytes and onion pathogen. All investigated strains share a great ability to produce bioactive secondary metabolites. Different authors have investigated this great capability and here we summarize the metabolic potential and the biological activities related to P. brasilianum’s metabolites with diverse structures. They include secondary metabolites of an alkaloid nature, i.e., 2,5-diketopiperazines, cyclodepsipeptides, meroterpenoids and polyketides. Penicillium brasilianum is also described as a great source of enzymes with biotechnological application potential, which is also highlighted in this review. Additionally, this review will focus on several aspects of Penicillium brasilianum and interesting genomic insights.

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