Endophytic Fungi as Potential Bio-Control Agents against Root Knot Nematode, Meloidogyne incognita in Banana

Ganeshan, K.; Vetrivelkalai, P.; Bhagawati, B.; Gupta, Nibha; Devrajan, K.; Raveendran, M.; Balachandar, D.

doi:10.9734/cjast/2021/v40i2931536

Cited by 4 publications

(1 citation statement)

References 26 publications

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“…Samples were brought to the laboratory of plant pathology and microbiology division, Regional Plant Resource Centre, Bhubaneswar and a surface sterilization procedure was followed [16,18,19] . A series of solvents were prepared like 70% ethanol, and 2.5% sodium hypo-chlorite along with sterile distilled water was also prepared.…”

Section: Methodsmentioning

confidence: 99%

Diversity of Endophytic Fungi in Banana Cultivars of Assam India

et al. 2023

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Endophytic fungal isolates (139 no.) were obtained from 143 (62 roots, 18 fruits and 54 leaves) samples of 15 different varieties of banana collected from 10 sites in Assam, India during 2018-2019. Overall isolation frequency from surface-sterilized tissue ranged from 10%-80% (as per site) and 6%-70% (as per variety of banana). All isolates were segregated into 40 different types on the basis of macromorphological and micro morphological characteristics. Forty different fungal taxa were isolated belonging to 14 genera including Absidia, Arthrinium, Aspergillus, Bipolaris, Cladosporium, Curvularia, Dendrophion, Fusarium, Humicola, Mortierella, Mucor, Penicillium, Paecilomyces, Verticillium and one mycelium sterile. Among them, Cladosporium cladosporioidies and Paecilomyces sp. frequently occurred in most of the sites surveyed whereas Cladospoirum cladosporioides and Aspergillus sp. 8, Fusarium graminseram were most frequently isolated from different varieties. However, all sites differed in their fungal diversity. Banana samples from Narigoan and Jorhat have been found with maximum fungal species followed by marigoan samples so as to Banana varieties Amrit Sagar endowed 27 no. of fungi followed by Jehaji and Honda which were associated with a maximum 14 fungal sp. Isolation frequency and relative abundance of Cladosporium cladosporiodes (80%, 4.6), Paecilomyces farinosus (80%, 4.6) followed by Penicillium ruburm, Aspergillus sp. 8 & 9 (70%, 4.02) were recorded as maximum comparatively in different sites. However, Aspergillus sp. 8, Mortieralla sp. and Pacilomyces farinosus are isolated frequently from different banana varieties (73.33%, 4.93).

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Section: Methodsmentioning

confidence: 99%

Diversity of Endophytic Fungi in Banana Cultivars of Assam India

et al. 2023

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Biocontrol efficacy of endophytic fungus, Acremonium sclerotigenum, against Meloidogyne incognita under in vitro and in vivo conditions

Yao,

Huo,

Ben

et al. 2023

Biologia

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Biocontrol microorganisms are important tools for the control of root knot nematodes (Meloidogyne spp.). Endophytic fungi have shown great potential as biocontrol agents in such applications. We here isolated an endophytic fungus from tomato root galls infected with M. incognita and identified the isolate as Acremonium sclerotigenum based on morphology and the internal transcribed spacer sequence. The biocontrol potential of this fungus was evaluated both in vitro and in vivo. Specifically, in vitro analyses were conducted to determine the potential of A. sclerotigenum to increase Meloidogyne incognita juvenile (J2 stage) mortality and decrease M. incognita egg hatching rates. The results revealed that A. sclerotigenum culture filtrates caused high J2 mortality rates (up to 95.5%) and significantly inhibited egg hatching (by up to ~ 43%). Furthermore, eggs treated with the culture filtrate were disaggregated and could not develop into nematodes. An in vivo experiment showed that treatment of tomato plants with A. sclerotigenum suppressed root knot nematode populations and significantly reduced the galling index. Both A. sclerotigenum treatment and exposure to the nematicide abamectin had good control effects, with efficacy rates of 55.43% and 70.58%, respectively. In summary, the endophytic fungus A. sclerotigenum here showed excellent potential for biocontrol of M. incognita. Further studies should be conducted to identify the nematicidal compounds produced by this fungus and to establish the molecular mechanism of action associated with the observed biocontrol effects.

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