Endophytic fungi from the medicinal plant Aloe dhufarensis Lavranos exhibit antagonistic potential against phytopathogenic fungi

Al-Rashdi, Fatma Khuseib Hamed; Al-Sadi, Abdullah M.; Al-Riyamy, Bahja Z.; Maharachchikumbura, Sajeewa S. N.; Al-Sabahi, Jamal Nasser; Velazhahan, R.

doi:10.1016/j.sajb.2020.05.022

Cited by 19 publications

(5 citation statements)

References 49 publications

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“…Dodecanoic acid, commonly called lauric acid, is a saturated fatty acid reported to have antagonistic activity against plant fungal pathogens, Pythium ultimum and Rhizoctonia solani ( Walters et al, 2003 ), Aspergillus niger ( Řiháková et al, 2001 ), and Fusarium spp. ( Al-Rashdi et al, 2020 ). Tetra decanoic acid, another saturated fatty acid commonly known as myristic acid was previously described for inhibitory activity on mycelial growth of Alternaria solani , F. oxysporum f. sp.…”

Section: Discussionmentioning

confidence: 99%

Decoding the Plant Growth Promotion and Antagonistic Potential of Bacterial Endophytes From Ocimum sanctum Linn. Against Root Rot Pathogen Fusarium oxysporum in Pisum sativum

2022

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The present study demonstrates plant growth promotion and induction of systemic resistance in pea (Pisum sativum) plant against Fusarium oxysporum f.sp. pisi by two bacterial endophytes, Pseudomonas aeruginosa OS_12 and Aneurinibacillus aneurinilyticus OS_25 isolated from leaves of Ocimum sanctum Linn. The endophytes were evaluated for their antagonistic potential against three phytopathogens Rhizoctonia solani, F. oxysporum f. sp. pisi, and Pythium aphanidermatum by dual culture assay. Maximum inhibition of F. oxysporum f. sp. pisi was observed by strains OS_12 and OS_25 among all root rot pathogens. Scanning electron microscopy of dual culture indicated hyphal distortion and destruction in the case of F. oxysporum f. sp. pisi. Further, volatile organic compounds (VOCs) were identified by gas chromatography–mass spectrometry (GC-MS). The GC-MS detected eight bioactive compounds from hexane extracts for instance, Dodecanoic acid, Tetra decanoic acid, L-ascorbic acid, Trans-13-Octadecanoic acid, Octadecanoic acid. Both the endophytes exhibited multifarious plant growth promoting traits such as indole acetic production (30–33 μg IAA ml–1), phosphate solubilization, and siderophore and ammonia production. Pot trials were conducted to assess the efficacy of endophytes in field conditions. A significant reduction in disease mortality rate and enhancement of growth parameters was observed in pea plants treated with consortium of endophytes OS_12 and OS_25 challenged with F. oxysporum f.sp. pisi infection. The endophytic strains elicited induced systemic resistance (ISR) in pathogen challenged pea plants by enhancing activities of Phenylalanine ammonia lyase (PAL), peroxidase (PO), polyphenol oxidase (PPO), ascorbate oxidase (AO), catalase (CAT) and total phenolic content. The endophytes reduced the oxidative stress as revealed by decrease in malondialdehyde (MDA) content and subsequently, lipid peroxidation in host plant leaves. Robust root colonization of pea seedlings by endophytes was observed by scanning electron microscopy (SEM) and fluorescence microscopy. Thus, plant growth promoting endophytic P. aeruginosa and A. aneurinilyticus can be further exploited through bio-formulations for sustainable protection of crops against root rot diseases as bio-control agents.

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Section: Discussionmentioning

confidence: 99%

Decoding the Plant Growth Promotion and Antagonistic Potential of Bacterial Endophytes From Ocimum sanctum Linn. Against Root Rot Pathogen Fusarium oxysporum in Pisum sativum

2022

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“…Then, the SPME was placed on the GC-MS for 7 min and 34 s, and then removed. The GC-MS was carried out using a Shimadzu GC-2010 Plus gas chromatography system equipped with an Rtx-5MS 30 m × 0.25 mm ID; 0.25 μm GC capillary column coupled to a GCMS-QP2010 ULTRA MS [ 69 ]. The analysis of the compounds was completed within 30 min.…”

Section: Methodsmentioning

confidence: 99%

Effectiveness of Endophytic and Rhizospheric Bacteria from Moringa spp. in Controlling Pythium aphanidermatum Damping-Off of Cabbage

Al-Rahbi

Al-Sadi

Al-Harrasi

et al. 2023

Plants

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In this study, endophytic and rhizospheric bacteria were isolated from Moringa olifera and M. perigreina from Oman, and their in vitro antagonistic activity against Pythium aphanidermatum was tested using a dual culture assay. The promising strains were tested further for their compatibility and potential for plant growth promotion, biofilm formation, antifungal volatile organic compound (VOC) production, and the biological control of P. aphanidermatum damping-off of cabbage (Brassica oleracea L.) under greenhouse conditions. A total of 12 endophytic and 27 rhizospheric bacteria were isolated from Moringa spp. Among them, Bacillus pumilus MPE1 showed the maximum antagonistic activity against P. aphanidermatum in the dual culture assay, followed by Paenibacillus glucanolyticus MPE3 and Pseudomonas indica MOR3 and MOR8. These bacterial isolates induced abundant morphological abnormalities in the hyphae of P. aphanidermatum, as observed via scanning electron microscopy. The in vitro cross-streak assay showed that these bacterial isolates were compatible among themselves, except for P. indica MOR8 × P. glucanolyticus MPE3. These antagonists released VOCs that restricted the growth of P. aphanidermatum in an in vitro assay. These antagonistic bacteria released 2,4-dimethylheptane and 4-methyloctane as the predominant volatile compounds. Of the four antagonistic bacterial strains, P. indica MOR8 was capable of forming biofilm, which is considered a trait that improves the efficacy of rhizosphere inoculants. The results of the greenhouse experiments showed that the soil treatment with B. pumilus MPE1 showed the highest reduction (59%) in the incidence of P. aphanidermatum damping-off in cabbage, evidencing its potential as a biological control agent for the management of this disease. Further research is needed to characterize the antifungal traits and activities of B. pumilus MPE1 and to assert its potential use against other soil-borne plant pathogens.

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“…Other than fungi and endophytic bacteria, Burkholderia pyrrocinia also inhibits the poplar canker pathogens Cytospora chrysosperma, Phomopsis macrospora, and Fusicoccum aesculin through the emission of volatile metabolites (dimethyl disulfide, benzothiazole, dimethylthiomethane, and phenylacetone) and improves the antioxidant defense system in vivo (Liu et al, 2020). Al-Rashdi et al (2022) documented the anti-phyto-pathogenic activity of fungal endophytes of Aloe dhufarensis-Sarocladium kiliense (SQUCC-F2-2) and Penicillium oxalicum (SQUCC-F3-1) against Fusarium sp. and Cladosporium sp., and the major antifungal metabolites were tetradecanoic acids, furfuryl alcohol, 1,2-diols, and amide, 2-furanmethanol, and dodecanoic acid.…”

Section: Discussionmentioning

confidence: 99%

Antifungal activity of volatile and non-volatile metabolites of endophytes of Chloranthus elatior Sw.

Santra¹,

Banerjee²

2023

Front. Plant Sci.

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Agriculture crops that have fungal infections suffer significant economic losses and reduced crop output. Chemical fungicides are used to tackle the problem, although this has additional detrimental side effects. There is an urgent need for safe and novel antifungals. Volatiles from plant-beneficial endophytic fungi are considered promising alternatives for the biological control of fungal pathogens as a sustainable approach in an agroecosystem. In the present investigation, a volatile-emitting sterile endophytic fungus, Diaporthe sp. CEL3 with bio-fumigation activity, was isolated from leaves of the ethnomedicinal plant Chloranthus elatior Sw., collected from the Passighat forest of North-East India. The camphor odor volatiles of CEL3 showed an inhibitory effect against eight fungal pathogens in vitro and minimized the infections of Monilinia fructicola, a causal agent of cherry fruit rot, in VOC-exposed cherry fruits. Rhizoctonia solani, Botrytis cinerea, Pythium ultimum, and M. fructicola were maximally inhibited up to 51.5%, 55.8%, 61.9%, and 78.5%, respectively, in comparison to control by the volatiles. Another isolate, CEL7, identified as Curvularia sp., synthesized non-volatile, soluble antifungal metabolites in its cell-free extracts and exhibited antifungal action. Bioassay-guided fractionation revealed the presence of imidazole compounds- (2-aminoethyl)-1H-imidazole-2-carbaldehyde, Pyrazole 4, 5 imidazole, 1-formyl 3-ethyl, phenol compounds-Phenol, 4-[2-(methylamino) ethyl]-, 6-Nitro-3-chlorophenol, Phenol, 2,4,6-tri-tert-butyl-, etc., in the cell-free extracts, with a MIC value of 250–2,000 µg ml−1. Optimum VOC emission was achieved in a modified PDA medium with instantly smashed potato (150 g L−1), dextrose (20 g L−1), wheat husk (20 g L−1), and yeast extract (20 g L−1), with additional salts. Interestingly, endophytic CEL3 emitted different types of volatiles, and trans-verbenol (32.25%), geraniol (30.32%), trans-ocimenol (12.90%), and mentha-4,8-diene (5.16%) were the prime ones. These VOCs cause lethal leakage of protein and necessary intracellular molecules from the fungal pathogens. Thus, CEL3 could potentially be used as a bio-fumigating agent to control post-harvest infections caused by fungal pathogens. This study opens a new approach to the use of endophytic fungi in biocontrol.

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Endophytic fungi from the medicinal plant Aloe dhufarensis Lavranos exhibit antagonistic potential against phytopathogenic fungi

Cited by 19 publications

References 49 publications

Decoding the Plant Growth Promotion and Antagonistic Potential of Bacterial Endophytes From Ocimum sanctum Linn. Against Root Rot Pathogen Fusarium oxysporum in Pisum sativum

Decoding the Plant Growth Promotion and Antagonistic Potential of Bacterial Endophytes From Ocimum sanctum Linn. Against Root Rot Pathogen Fusarium oxysporum in Pisum sativum

Effectiveness of Endophytic and Rhizospheric Bacteria from Moringa spp. in Controlling Pythium aphanidermatum Damping-Off of Cabbage

Antifungal activity of volatile and non-volatile metabolites of endophytes of Chloranthus elatior Sw.

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