Endophytic Fungi in the Fight Against Neglected Tropical Diseases

Costa, Maria Beatriz Silva; Oliveira, Camila Martins de

doi:10.2174/1389557520666200624193300

Cited by 2 publications

(1 citation statement)

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“…Owing to the resistance of mosquitoes and the low effectiveness and high cost of programs to control the vectors of these diseases [8][9][10] , new strategies are needed to combat vectors using mechanisms that are less polluting, less toxic, and pose less risk to human health 11,12 . Some studies involving natural bioactive products made from endophytic fungi have revealed promising alternatives 13 .…”

Section: Introductionmentioning

confidence: 99%

Oxidative stress induction by crude extract of Xylaria sp. triggers lethality in the larvae of Aedes aegypti (Diptera: Culicidae)

Costa

Simões

Silva

et al. 2022

Rev. Soc. Bras. Med. Trop.

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Background: Aedes aegypti is currently controlled with synthetic larvicides; however, mosquitoes have become highly resistant to these larvicides and difficult to eradicate. Studies have shown that insecticides derived from fungal extracts have various mechanisms of action that reduce the risk of resistance in these mosquitoes. One possible mechanism is uncontrolled production of reactive oxygen species (ROS) in the larvae, which can cause changes at the cellular level. Thus, the crude extract of Xylaria sp. was evaluated to investigate the oxidative effect of this extract in A. aegypti larvae by quantifying the oxidative damage to proteins and lipids. Methods:The larvicidal potential of the crude extract of Xylaria sp. Was evaluated, and the extract was subsequently tested in human lung fibroblasts for cytotoxicity and ROS production. ROS level was quantified in the larvae that were killed following exposure to the extract in the larvicide test. Results:The crude extract of Xylaria sp. Caused cytotoxicity and induced ROS production in human lung fibroblasts and A. aegypti larvae, respectively. In the larvicide trial, the extract showed an LC 50 of 264.456 ppm and an LC90 of 364.307 ppm, and was thus considered active. The extract showed greater oxidative damage to lipids and proteins, with LC 90 values of 24.7 µmol MDA/L and 14.6278 ×10 -3 nmol carbonyl/ mg protein, respectively.Conclusions: Crude extracts of Xylaria sp. induced oxidative stress that may have caused the mortality of A. aegypti larvae.

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Section: Introductionmentioning

confidence: 99%