Endophytic
            <i>Streptomyces hygroscopicus</i>
            OsiSh-2-Mediated Balancing between Growth and Disease Resistance in Host Rice

Gao, Yan; Ning, Qing; Yang, Yuanzhu; Liu, Ying; Niu, Shuqi; Hu, Xiaochun; Pan, Huairong; Bu, Zhigang; Chen, Ning; Guo, Jinyou; Yu, Jin; Cao, Lidan; Qin, Peng; Xing, Junjie; Liu, Bin; Liu, Xuanming; Zhu, Yonghua

doi:10.1128/mbio.01566-21

Cited by 28 publications

(32 citation statements)

References 55 publications

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“…As our previous study showed that OsiSh-2 inoculation could induce host resistance to rice blast while still maintaining high growth, 29 we also investigated the growth of rice under M. oryzae stress. Consistent with the previous results, the total chlorophyll content in E+ rice was significantly increased by 33.3% compared with that in E− rice under M. oryzae infection (Figure 8C).…”

Section: ■ Resultsmentioning

confidence: 99%

“…However, the WT strain showed cellular homeostasis with the host rice and maintained a balance of growth and defense. 29 The WT strain without growth defects required less biomass to reach this symbiotic status with the host, thus explaining why the biomass of WT was lower than that of ΔTHIC in rice. In addition, since WT could provide thiamine to rice, the rice could utilize the energy saved by reducing thiamine synthetic levels to maintain an active growth status, even during M. oryzae invasion (Figure 8C−G).…”

Section: ■ Discussionmentioning

confidence: 99%

“…In our previous study, we obtained the rice endophytic Streptomyces hygroscopicus OsiSh-2 (hereafter OsiSh-2), which exhibited remarkable antagonistic activity against rice blast and maintained plant growth under laboratory and field conditions. 28,29 Further research revealed that siderophores secreted by OsiSh-2 could inhibit the growth of M. oryzae by capturing iron, 30 and nigericin might be one of the antifungal antibiotics produced by OsiSh-2. 28 However, our understanding of the molecular mechanism of the OsiSh-2-produced active compound involved in disease control is still in its infancy.…”

Section: ■ Introductionmentioning

confidence: 99%

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The Vital Role of ShTHIC from the Endophyte OsiSh-2 in Thiamine Biosynthesis and Blast Resistance in the OsiSh-2-Rice Symbiont

Liu

Qin

Chen

et al. 2022

J. Agric. Food Chem.

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Endophytes can benefit the growth and stress resistance of host plants by secreting bioactive components. Thiamine is an essential vitamin involved in many metabolic pathways and can only be synthesized by microbes and plants. In this study, we found that thiamine could inhibit the development of the phytopathogen Magnaporthe oryzae and decrease the rice blast index under field conditions. In the thiamine biosynthesis pathway, the key enzyme ShTHIC of an endophyte Streptomyces hygroscopicus OsiSh-2 and OsTHIC of rice (Oryza sativa) were highly homologous. Gene overexpression or knockout approaches revealed that both THIC contributed to thiamine synthesis and resistance to M. oryzae. Furthermore, S. hygroscopicus OsiSh-2 colonization led to a decrease in the thiamine synthesis level of rice but still maintained thiamine homeostasis in rice. However, inoculation with the ShTHIC knockout strain ΔTHIC reduced the thiamine content in rice, although the thiamine synthesis level of rice was increased. After infection with M. oryzae, blast resistance was dramatically improved in OsiSh-2-inoculated rice but decreased in ΔTHIC-inoculated rice compared with non-inoculated rice. This result demonstrated that ShTHIC could regulate thiamine biosynthesis and consequently assist blast resistance in the OsiSh-2-rice symbiont. Our results revealed a novel blast-resistance mechanism mediated by a key thiamine biosynthetic enzyme from an endophyte OsiSh-2.

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Section: ■ Resultsmentioning

confidence: 99%

Section: ■ Discussionmentioning

confidence: 99%

Section: ■ Introductionmentioning

confidence: 99%

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The Vital Role of ShTHIC from the Endophyte OsiSh-2 in Thiamine Biosynthesis and Blast Resistance in the OsiSh-2-Rice Symbiont

Liu

Qin

Chen

et al. 2022

J. Agric. Food Chem.

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“…Strain PRh5 [48], Streptomyces sp. 11-1-2 [29,49], S. hygroscopicus Osish-2 [50][51][52][53][54], Streptomyces sp. NBRC 109436 [55] and S. rhizosphaericus NRRL B-24304 [32] were isolated from the rhizosphere of diverse plants across the world.…”

Section: Chromosome-scale Assembly Of Agn23 and Genome-based Taxonomi...mentioning

confidence: 99%

Genomic adaptation in the CAZyome and specialised metabolism of the plant-associated Streptomyces violaceusniger clade

Gayrard

Veyssière

Adam

et al. 2021

Preprint

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Streptomycetes are Gram-positive actinobacteria largely represented in the plant root microbiota. The genetic determinants involved in the presence of Streptomyces in the rhizosphere are largely unknown and can rely on the ability to degrade plant-derived compounds such as cell-wall polysaccharides and on the production of specialised metabolites. To address whether Streptomyces strains recruited into root microbiota share genomic specificities related to these two functions, we engaged a comparative genomic analysis using a newly sequenced rhizospheric strain, Streptomyces sp. AgN23 and strains from the phylogenetically related S. violaceusniger clade. This analysis enlightens a shared prominent CAZyome potentially involved in plant polysaccharides degradation and a strong conservation of antimicrobials biosynthetic clusters (rustmicin, mediomycin, niphimycin, nigericin) as well as plant bioactive compounds (nigericin, echosides, elaiophylin). Taken together, our work supports the hypothesis that specific hydrolytic enzymes and specialised metabolites repertoires may play important roles in the development of Streptomyces strains in the rhizosphere.

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“…Compared with soil microorganisms, endophytes within plants may have more positive and direct impacts on plants because of their special ecological niches. The results from different research teams have provided good evidence for the effects of endophytes on plant growth promotion, stress mitigation, and disease resistance in host plants [ 9 , 10 , 11 , 12 ]. The mechanisms proposed for disease prevention include competition with pathogens for niche and nutrition, induction of plant resistance, secretion of bioactive metabolites, and promotion of plant growth, usually working in concert [ 13 , 14 , 15 ].…”

Section: Introductionmentioning

confidence: 99%

The Multifunctions and Future Prospects of Endophytes and Their Metabolites in Plant Disease Management

et al. 2022

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Endophytes represent a ubiquitous and magical world in plants. Almost all plant species studied by different researchers have been found to harbor one or more endophytes, which protect host plants from pathogen invasion and from adverse environmental conditions. They produce various metabolites that can directly inhibit the growth of pathogens and even promote the growth and development of the host plants. In this review, we focus on the biological control of plant diseases, aiming to elucidate the contribution and key roles of endophytes and their metabolites in this field with the latest research information. Metabolites synthesized by endophytes are part of plant disease management, and the application of endophyte metabolites to induce plant resistance is very promising. Furthermore, multi-omics should be more fully utilized in plant–microbe research, especially in mining novel bioactive metabolites. We believe that the utilization of endophytes and their metabolites for plant disease management is a meaningful and promising research direction that can lead to new breakthroughs in the development of more effective and ecosystem-friendly insecticides and fungicides in modern agriculture.

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Endophytic Streptomyces hygroscopicus OsiSh-2-Mediated Balancing between Growth and Disease Resistance in Host Rice

Cited by 28 publications

References 55 publications

The Vital Role of ShTHIC from the Endophyte OsiSh-2 in Thiamine Biosynthesis and Blast Resistance in the OsiSh-2-Rice Symbiont

The Vital Role of ShTHIC from the Endophyte OsiSh-2 in Thiamine Biosynthesis and Blast Resistance in the OsiSh-2-Rice Symbiont

Genomic adaptation in the CAZyome and specialised metabolism of the plant-associated Streptomyces violaceusniger clade

The Multifunctions and Future Prospects of Endophytes and Their Metabolites in Plant Disease Management

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