Endophytic Microbiomes and Their Plant Growth-Promoting Attributes for Plant Health

Ghosh, Sougata; Bhagwat, Tanay; Webster, Thomas J.

doi:10.1007/978-981-15-6949-4_11

Cited by 13 publications

(5 citation statements)

References 113 publications

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“…As reported in a previous study (Rodriguez et al, 2019 ; Fadiji et al, 2020 ; Ghosh et al, 2021 ), many bacterial isolates (i.e., Bacillus, Paenibacillus, Rhizobium, Pseudomonas, Streptomyces, Sphingobium, Burkholderia , and Chitinophaga ) within the culture collections may have plant growth-promoting attributes and interact with complex eukaryotes. These plant growth-promoting strains are well-known for nitrogen fixation, solubilizing phosphorus, siderophore production, phytohormone production, antibiotics production, or systemic resistance.…”

Section: Discussionmentioning

confidence: 62%

Enriching the endophytic bacterial microbiota of Ginkgo roots

et al. 2023

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Bacterial endophytes of Ginkgo roots take part in the secondary metabolic processes of the fossil tree and contribute to plant growth, nutrient uptake, and systemic resistance. However, the diversity of bacterial endophytes in Ginkgo roots is highly underestimated due to the lack of successful isolates and enrichment collections. The resulting culture collection contains 455 unique bacterial isolates representing 8 classes, 20 orders, 42 families, and 67 genera from five phyla: Actinobacteria, Bacteroidetes, Firmicutes, Proteobacteria, and Deinococcus-Thermus, using simply modified media (a mixed medium without any additional carbon sources [MM)] and two other mixed media with separately added starch [GM] and supplemented glucose [MSM]). A series of plant growth-promoting endophytes had multiple representatives within the culture collection. Moreover, we investigated the impact of refilling carbon sources on enrichment outcomes. Approximately 77% of the natural community of root-associated endophytes were predicted to have successfully cultivated the possibility based on a comparison of the 16S rRNA gene sequences between the enrichment collections and the Ginkgo root endophyte community. The rare or recalcitrant taxa in the root endosphere were mainly associated with Actinobacteria, Alphaproteobacteria, Blastocatellia, and Ktedonobacteria. By contrast, more operational taxonomic units (OTUs) (0.6% in the root endosphere) became significantly enriched in MM than in GM and MSM. We further found that the bacterial taxa of the root endosphere had strong metabolisms with the representative of aerobic chemoheterotrophy, while the functions of the enrichment collections were represented by the sulfur metabolism. In addition, the co-occurrence network analysis suggested that the substrate supplement could significantly impact bacterial interactions within the enrichment collections. Our results support the fact that it is better to use the enrichment to assess the cultivable potential and the interspecies interaction as well as to increase the detection/isolation of certain bacterial taxa. Taken together, this study will deepen our knowledge of the indoor endophytic culture and provide important insights into the substrate-driven enrichment.

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Section: Discussionmentioning

confidence: 62%

Enriching the endophytic bacterial microbiota of Ginkgo roots

et al. 2023

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“…Induction of systemic resistance is the immunity response mechanism in plants that can be triggered by endophytic bacteria during abiotic stress conditions, including heavy metal (Ghosh et al, 2020). The relative expression level of the NPR3 gene in alfalfa and chickpea plants treated with endophytic bacteria in soil supplemented with 50-and 100mg kg −1 concentrations of As, respectively, was analyzed.…”

Section: Discussionmentioning

confidence: 99%

Characterization of Arsenic-Resistant Endophytic Bacteria From Alfalfa and Chickpea Plants

et al. 2021

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The present investigation was carried out to isolate arsenic (As)-resistant endophytic bacteria from the roots of alfalfa and chickpea plants grown in arsenic-contamination soil, characterize their As tolerance ability, plant growth-promoting characteristics, and their role to induce As resistance by the plant. A total of four root endophytic bacteria were isolated from plants grown in As-contaminated soil (160–260-mg As kg−1 of soil). These isolates were studied for plant growth-promoting (PGP) characteristics through siderophore, phosphate solubilization, nitrogen fixation, protease, and lipase production, and the presence of the arsenate reductase (arsC) gene. Based on 16S rDNA sequence analysis, these isolates belong to the genera Acinetobacter, Pseudomonas, and Rahnella. All isolates were found As tolerant, of which one isolate, Pseudomonas sp. QNC1, showed the highest tolerance up to 350-mM concentration in the LB medium. All isolates exhibited phosphate solubilization activity. Siderophore production activity was shown by only Pseudomonas sp. QNC1, while nitrogen fixation activity was shown by only Rahnella sp. QNC2 isolate. Acinetobacter sp. QNA1, QNA2, and Rahnella sp. QNC2 exhibited lipase production, while only Pseudomonas sp. QNC1 was able to produce protease. The presence of the arsC gene was detected in all isolates. The effect of endophytic bacteria on biomass production of alfalfa and chickpea in five levels of arsenic concentrations (0-, 10-, 50-, 75-, and 100-mg kg−1 soil) was evaluated. The fresh and dry weights of roots of alfalfa and chickpea plants were decreased as the arsenic concentration of the soil was increased. Results indicate that the fresh and dry root weights of alfalfa and chickpea plants were significantly higher in endophytic bacteria-treated plants compared with non-treated plants. Inoculation of chickpea plants with Pseudomonas sp. QNC1 and Rahnella sp. QNC2 induced lower NPR3 gene expression in chickpea roots grown in soil with the final concentration of 100-mg kg−1 sodium arsenate compared with the non-endophyte-treated control. The same results were obtained in Acinetobacter sp. QNA2-treated alfalfa plants grown in the soil plus 50-mg kg−1 sodium arsenate. These results demonstrated that arsenic-resistant endophytic bacteria are potential candidates to enhance plant-growth promotion in As contamination soils. Characterization of bacterial endophytes with plant growth potential can help us apply them to improve plant yield under stress conditions.

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“…Among them, Actinobacteria, Bacillus, Enterobacter, Paenibacillus, Pseudomonas and other bacteria are common genera with antibacterial activity against plant pathogens (Afzal et al, 2019). Some of the enzymes produced by PGPB, including chitinase, cellulase, glucanase, protease and lipase, can lyse a portion of the cell walls of pathogens (Ghosh et al, 2021). PGPB that secrete one or more of these enzymes have been shown to have biocontrol activities against a wide range of competitor pathogens (Selari et al, 2023).…”

Section: Inhibition Of Plant Pathogensmentioning

confidence: 99%

From salty to thriving: plant growth promoting bacteria as nature’s allies in overcoming salinity stress in plants

et al. 2023

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Soil salinity is one of the main problems that affects global crop yield. Researchers have attempted to alleviate the effects of salt stress on plant growth using a variety of approaches, including genetic modification of salt-tolerant plants, screening the higher salt-tolerant genotypes, and the inoculation of beneficial plant microbiome, such as plant growth-promoting bacteria (PGPB). PGPB mainly exists in the rhizosphere soil, plant tissues and on the surfaces of leaves or stems, and can promote plant growth and increase plant tolerance to abiotic stress. Many halophytes recruit salt-resistant microorganisms, and therefore endophytic bacteria isolated from halophytes can help enhance plant stress responses. Beneficial plant-microbe interactions are widespread in nature, and microbial communities provide an opportunity to understand these beneficial interactions. In this study, we provide a brief overview of the current state of plant microbiomes and give particular emphasis on its influence factors and discuss various mechanisms used by PGPB in alleviating salt stress for plants. Then, we also describe the relationship between bacterial Type VI secretion system and plant growth promotion.

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Endophytic Microbiomes and Their Plant Growth-Promoting Attributes for Plant Health

Cited by 13 publications

References 113 publications

Enriching the endophytic bacterial microbiota of Ginkgo roots

Enriching the endophytic bacterial microbiota of Ginkgo roots

Characterization of Arsenic-Resistant Endophytic Bacteria From Alfalfa and Chickpea Plants

From salty to thriving: plant growth promoting bacteria as nature’s allies in overcoming salinity stress in plants

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