Endophytic microorganisms Agrobacterium tumefaciens 6N2 and Meyerozyma guilliermondii 6N serve as models for the study of microbial interactions in colony biofilms

Bertini, Elisa Violeta; Leguina, Ana Carolina del Valle; Figueroa, Lucía I. C. de; Nieto-Peñalver, Carlos Gabriel

doi:10.1016/j.ram.2018.09.006

Cited by 3 publications

(3 citation statements)

References 2 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…During a previous survey of the endophytic microbiota of sugarcane (Saccharum officinarum L.), we isolated the yeast Meyerozyma guilliermondii strain 6N and A. tumefaciens strain 6N2 from the same node section, suggesting that these two microorganisms can co-occupy this niche and, in consequence, interact with each other [11,12]. In contrast to other species, this M. guilliermondii isolate show a very weak capacity to inactivate AHLs [10].…”

Section: Introductionmentioning

confidence: 90%

Insight in the quorum sensing-driven lifestyle of the non-pathogenic Agrobacterium tumefaciens 6N2 and the interactions with the yeast Meyerozyma guilliermondii

Bertini

Torres

Léger

et al. 2021

Preprint

Self Cite

View full text Add to dashboard Cite

Agrobacterium tumefaciens is considered a prominent phytopathogen, though most isolates are nonpathogenic. Agrobacteria can inhabit plant tissues interacting with other microorganisms. Yeasts are likewise part of these communities. We analyzed the quorum sensing (QS) systems of A. tumefaciens strain 6N2, and its relevance for the interaction with the yeast Meyerozyma guilliermondii, both sugarcane endophytes. We show that strain 6N2 is nonpathogenic, produces OHC8-HSL, OHC10-HSL, OC12-HSL and OHC12-HSL as QS signals, and possesses a complex QS architecture, with one truncated, two complete systems, and three additional QS-signal receptors. A proteomic approach showed differences in QS-regulated proteins between pure (64 proteins) and dual (33 proteins) cultures. Seven proteins were consistently regulated by quorum sensing in pure and dual cultures. M. guilliermondii proteins influenced by QS activity were also evaluated. Several up- and down- regulated proteins differed depending on the bacterial QS. These results show the importance of the QS regulation in the bacteria-yeast interactions.

show abstract

Section: Introductionmentioning

confidence: 90%

Insight in the quorum sensing-driven lifestyle of the non-pathogenic Agrobacterium tumefaciens 6N2 and the interactions with the yeast Meyerozyma guilliermondii

Bertini

Torres

Léger

et al. 2021

Preprint

Self Cite

View full text Add to dashboard Cite

show abstract

“…The incorporation of yeast into the packaging material could impart antimicrobial activity as well as enhancing its nutritional value and serving as the probiotic. For instance, the yeast Meyerozyma guilliermondii is non-pathogenic and demonstrates good antimicrobial activity and it is a rich source of vitamins and proteins [ 123 ]. In 2013, Coda and colleagues [ 124 ] demonstrated the efficacy of M. guilliermondii strain LCF1353 for the effective antifungal activity on long-term storage in wheat bread.…”

Section: Antimicrobial Activity Of Non- Saccharomyces ...mentioning

confidence: 99%

Biocontrol and Probiotic Function of Non-Saccharomyces Yeasts: New Insights in Agri-Food Industry

et al. 2023

View full text Add to dashboard Cite

Fermented food matrices, including beverages, can be defined as the result of the activity of complex microbial ecosystems where different microorganisms interact according to different biotic and abiotic factors. Certainly, in industrial production, the technological processes aim to control the fermentation to place safe foods on the market. Therefore, if food safety is the essential prerogative, consumers are increasingly oriented towards a healthy and conscious diet driving the production and consequently the applied research towards natural processes. In this regard, the aim to guarantee the safety, quality and diversity of products should be reached limiting or avoiding the addition of antimicrobials or synthetic additives using the biological approach. In this paper, the recent re-evaluation of non-Saccharomyces yeasts (NSYs) has been reviewed in terms of bio-protectant and biocontrol activity with a particular focus on their antimicrobial power using different application modalities including biopackaging, probiotic features and promoting functional aspects. In this review, the authors underline the contribution of NSYs in the food production chain and their role in the technological and fermentative features for their practical and useful use as a biocontrol agent in food preparations.

show abstract

“…The incorporation of yeast into the packaging material could impart antimicrobial activity as well as enhancing its nutritional value and serving as the probiotic. For instance, the yeast Meyerozyma guilliermondii is non-pathogenic and demonstrates good antimicrobial activity, and is a rich source of vitamins and proteins [ 32 , 33 ]. The direct incorporation of M. guilliermondii or its metabolites to the packaging material imparts it with antimicrobial activity, and thus contributes to increasing the shelf life of food [ 34 , 35 ].…”

Section: Introductionmentioning

confidence: 99%

Development and Characterization of Yeast-Incorporated Antimicrobial Cellulose Biofilms for Edible Food Packaging Application

et al. 2021

View full text Add to dashboard Cite

The unique properties and advantages of edible films over conventional food packaging have led the way to their extensive exploration in recent years. Moreover, the incorporation of bioactive components during their production has further enhanced the intrinsic features of packaging materials. This study was aimed to develop edible and bioactive food packaging films comprising yeast incorporated into bacterial cellulose (BC) in conjunction with carboxymethyl cellulose (CMC) and glycerol (Gly) to extend the shelf life of packaged food materials. First, yeast biomass and BC hydrogels were produced by Meyerozyma guilliermondii (MT502203.1) and Gluconacetobacter xylinus (ATCC53582), respectively, and then the films were developed ex situ by mixing 30 wt.% CMC, 30 wt.% Gly, 2 wt.% yeast dry biomass, and 2 wt.% BC slurry. FE-SEM observation showed the successful incorporation of Gly and yeast into the fibrous cellulose matrix. FTIR spectroscopy confirmed the development of composite films through chemical interaction between BC, CMC, Gly, and yeast. The developed BC/CMC/Gly/yeast composite films showed high water solubility (42.86%). The yeast-incorporated films showed antimicrobial activities against three microbial strains, including Escherichia coli, Pseudomonas aeruginosa, and Saccharomyces aureus, by producing clear inhibition zones of 16 mm, 10 mm, and 15 mm, respectively, after 24 h. Moreover, the films were non-toxic against NIH-3T3 fibroblast cells. Finally, the coating of oranges and tomatoes with BC/CMC/Gly/yeast composites enhanced the shelf life at different storage temperatures. The BC/CMC/Gly/yeast composite film-coated oranges and tomatoes demonstrated acceptable sensory features such as odor and color, not only at 6 °C but also at room temperature and further elevated temperatures at 30 °C and 40 °C for up to two weeks. The findings of this study indicate that the developed BC/CMC/Gly/yeast composite films could be used as edible packaging material with high nutritional value and distinctive properties related to the film component, which would provide protection to foods and extend their shelf life, and thus could find applications in the food industry.

show abstract

Endophytic microorganisms Agrobacterium tumefaciens 6N2 and Meyerozyma guilliermondii 6N serve as models for the study of microbial interactions in colony biofilms

Cited by 3 publications

References 2 publications

Insight in the quorum sensing-driven lifestyle of the non-pathogenic Agrobacterium tumefaciens 6N2 and the interactions with the yeast Meyerozyma guilliermondii

Insight in the quorum sensing-driven lifestyle of the non-pathogenic Agrobacterium tumefaciens 6N2 and the interactions with the yeast Meyerozyma guilliermondii

Biocontrol and Probiotic Function of Non-Saccharomyces Yeasts: New Insights in Agri-Food Industry

Development and Characterization of Yeast-Incorporated Antimicrobial Cellulose Biofilms for Edible Food Packaging Application

Contact Info

Product

Resources

About