Endophytic Trichoderma citrinoviride isolated from mountain-cultivated ginseng (Panax ginseng) has great potential as a biocontrol agent against ginseng pathogens

Park, Young Hwan; Mishra, Ratnesh Chandra; Yoon, Sunkyung; Kim, Hoki; Park, Chang-Ho; Seo, Sang‐Tae; Bae, Hyeun‐Jong

doi:10.1016/j.jgr.2018.03.002

Cited by 93 publications

(33 citation statements)

References 58 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Generally, secondary metabolites synthesized by the BEs help the host plants fight and protect against the pathogens 22 or from any abiotic stress 23 . Several antimicrobial 13 , antifungal 11 and antinematicidal 24 activities of such secondary metabolites have been well documented 25 . Due to existing evidence of these inhibitory abilities, we investigated the antinematicidal activity of diverse BEs isolated from the four Pinus species.…”

Section: Discussionmentioning

confidence: 99%

“…More recently, studies focusing on the use of BEs as biocontrol agents have shown a promising glimpse of their remedial application in the agriculture sector 10 . In general, BEs colonizing host plants have the tendency to demonstrate antagonistic activity against their own or different plant pathogens 11,12 . These beneficial activities are due to the production of secondary metabolites by BEs, many of which are used for various applications 13 .…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Phylogenetic characterization of bacterial endophytes from four Pinus species and their nematicidal activity against the pine wood nematode

Ponpandian

Rim

Shanmugam

et al. 2019

Sci Rep

Self Cite

View full text Add to dashboard Cite

Recently, bacterial endophytes (BEs) have gained importance in the agricultural sector for their use as biocontrol agents to manage plant pathogens. Outbreak of the pine wilt disease (PWD) in Korea has led researchers to test the feasibility of BEs in controlling the pine wood nematode (PWN) Bursaphelenchus xylophilus . In this study, we have reported the diversity and biocontrol activity of BEs against the PWN. By employing a culture-dependent approach, 1,622 BEs were isolated from the needle, stem, and root tissues of P . densiflora , P . rigida , P . thunbergii , and P . koraiensis across 18 sampling sites in Korea. We classified 389 members based on 16S rDNA analysis and taxonomic binning, of which, 215 operational taxonomic units (OTUs) were determined. Using Shannon’s indices, diversity across the Pinus species and tissues was estimated to reveal the composition of BEs and their tissue-specific preferences. When their ethyl acetate crude extracts were analysed for biocontrol activity, 44 candidates with nematicidal activity were obtained. Among these, Stenotrophomonas and Bacillus sp. exhibited significant inhibitory activity against PWN during their developmental stages. Altogether, our study furnishes a basic comprehension of bacterial communities found in the Pinus species and highlights the potential of BEs as biocontrol agents to combat PWD.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Phylogenetic characterization of bacterial endophytes from four Pinus species and their nematicidal activity against the pine wood nematode

Ponpandian

Rim

Shanmugam

et al. 2019

Sci Rep

Self Cite

View full text Add to dashboard Cite

show abstract

“…The presence of these traits predicted the outcome of a three-partite interaction among a host, a pathogen, and an endophyte mutualist. Various Trichoderma species and strains isolated as endophytes and characterized as having mycotrophic traits in vitro (dual assays) were later inoculated into host plants, successfully reducing the presence of a fungal plant pathogen and consequently reducing and/or preventing disease in the host (Pujade-Renaud et al (2019); Lahlali & Hijri, 2010;Park et al, 2019). As fungal traits are measured in the laboratory and tested in the field, their utility can be increased by documenting experimental conditions and cataloguing fungal strains for future.…”

Section: Size-related Scalingmentioning

confidence: 99%

Fungal functional ecology: bringing a trait‐based approach to plant‐associated fungi

et al. 2019

View full text Add to dashboard Cite

Fungi play many essential roles in ecosystems. They facilitate plant access to nutrients and water, serve as decay agents that cycle carbon and nutrients through the soil, water and atmosphere, and are major regulators of macro‐organismal populations. Although technological advances are improving the detection and identification of fungi, there still exist key gaps in our ecological knowledge of this kingdom, especially related to function. Trait‐based approaches have been instrumental in strengthening our understanding of plant functional ecology and, as such, provide excellent models for deepening our understanding of fungal functional ecology in ways that complement insights gained from traditional and ‐omics‐based techniques. In this review, we synthesize current knowledge of fungal functional ecology, taxonomy and systematics and introduce a novel database of fungal functional traits (FunFun). FunFun is built to interface with other databases to explore and predict how fungal functional diversity varies by taxonomy, guild, and other evolutionary or ecological grouping variables. To highlight how a quantitative trait‐based approach can provide new insights, we describe multiple targeted examples and end by suggesting next steps in the rapidly growing field of fungal functional ecology.

show abstract

“…T. reesei is a well-studied saprobe and used industrially for the production of cellulases and hemicellulases [12]. T. harzianum is a ubiquitous species with agricultural applications, the opportunistically pathogenic T. citrinoviride is often isolated as endophyte and T. brevicompactum is a producer of antifungal metabolites [12,[19][20][21]. All mentioned Trichoderma species contain various classes of BGCs, Type 1 polyketide synthetases (T1pks), nonribosomal peptide synthases (NRPSs), terpene BGCs, fatty acid BGCs and various combined and putative clusters.…”

Section: Introductionmentioning

confidence: 99%

Novel approach in whole genome mining and transcriptome analysis reveal conserved RiPPs in Trichoderma spp

et al. 2020

View full text Add to dashboard Cite

Background: Ribosomally synthesized and post-translationally modified peptides (RiPPs) are a highly diverse group of secondary metabolites (SM) of bacterial and fungal origin. While RiPPs have been intensively studied in bacteria, little is known about fungal RiPPs. In Fungi only six classes of RiPPs are described. Current strategies for genome mining are based on these six known classes. However, the genes involved in the biosynthesis of theses RiPPs are normally organized in biosynthetic gene clusters (BGC) in fungi. Results: Here we describe a comprehensive strategy to mine fungal genomes for RiPPs by combining and adapting existing tools (e.g. antiSMASH and RiPPMiner) followed by extensive manual curation based on conserved domain identification, (comparative) phylogenetic analysis, and RNASeq data. Deploying this strategy, we could successfully rediscover already known fungal RiPPs. Further, we analysed four fungal genomes from the Trichoderma genus. We were able to find novel potential RiPP BGCs in Trichoderma using our unconventional mining approach. Conclusion: We demonstrate that the unusual mining approach using tools developed for bacteria can be used in fungi, when carefully curated. Our study is the first report of the potential of Trichoderma to produce RiPPs, the detected clusters encode novel uncharacterized RiPPs. The method described in our study will lead to further mining efforts in all subdivisions of the fungal kingdom.

show abstract

Endophytic Trichoderma citrinoviride isolated from mountain-cultivated ginseng (Panax ginseng) has great potential as a biocontrol agent against ginseng pathogens

Cited by 93 publications

References 58 publications

Phylogenetic characterization of bacterial endophytes from four Pinus species and their nematicidal activity against the pine wood nematode

Phylogenetic characterization of bacterial endophytes from four Pinus species and their nematicidal activity against the pine wood nematode

Fungal functional ecology: bringing a trait‐based approach to plant‐associated fungi

Novel approach in whole genome mining and transcriptome analysis reveal conserved RiPPs in Trichoderma spp

Contact Info

Product

Resources

About