Endoplasmic Reticulum-Associated Secretory Proteins Sec20p, Sec39p, and Dsl1p Are Involved in Peroxisome Biogenesis

Perry, Ryan J.; Mast, Fred D.; Rachubinski, Richard A.

doi:10.1128/ec.00024-09

Cited by 69 publications

(65 citation statements)

References 76 publications

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“…5B). However, this time Pex3 was unaffected in its migration by Mg 2ϩ concentration and was found exclusively in fractions of heavy density where it cofractionated with the oleic acid-induced peroxisomal matrix protein Pot1, as has been demonstrated previously (88). As demonstrated by Erdmann and Blobel (29), peroxisome density is increased in yeast grown in the presence of oleic acid because of the additional import of peroxisomal matrix proteins involved in ␤-oxidation.…”

Section: Er-dependent Regulation Of Peroxisome Biogenesissupporting

confidence: 73%

Peroxins Pex30 and Pex29 Dynamically Associate with Reticulons to Regulate Peroxisome Biogenesis from the Endoplasmic Reticulum

Mast

Jamakhandi

Saleem

et al. 2016

Journal of Biological Chemistry

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Peroxisome proliferation occurs by at least two routes, division of existing peroxisomes and de novo biogenesis from the endoplasmic reticulum (ER). The proteins and molecular mechanisms governing peroxisome emergence from the ER are poorly characterized. In this study, we report that two integral membrane peroxins (proteins required for peroxisome biogenesis) in Saccharomyces cerevisiae, Pex29 and Pex30, reside in distinct regions of the ER and associate with Rtn1 and Yop1, reticulon family members that contribute to ER morphology, to govern peroxisome emergence from the ER. In vivo and in vitro analyses reveal that peroxisome proliferation is therefore not restricted to the peroxisome but begins at the level of the ER.Peroxisomes are organelles found across the diversity of eukaryotes. Bound by a single lipid bilayer, peroxisomes contain enzymes involved in lipid metabolism that are coupled to the production of hydrogen peroxide and the scavenging of reactive oxygen species. Peroxisome number, size, and volume are dynamically linked to cell type, its developmental state, and environmental stimuli. Control of these processes is critical to human health. Defects in peroxisome membrane formation, protein import, and organelle proliferation cause severe pathologies in humans, and peroxisome function is linked to numerous human health issues (1-4). Studies in many different model systems, including notably different species of yeast, have led to the identification of peroxins, which are defined as proteins involved in peroxisome biogenesis (5). The mechanisms of action of the more than 30 peroxins in peroxisomal biogenesis and proliferation are the subject of intense investigation.There has been much debate as to whether peroxisomes are semi-autonomous organelles like mitochondria or are derived from internal membrane systems such as the secretory pathway. Evidence supports that peroxisome biogenesis occurs through two separate pathways, de novo biogenesis in which new peroxisomes bud from the ER 3 and the division of existing peroxisomes (6 -12). These biogenesis pathways are tightly regulated spatially and temporally and involve a host of molecular interactions that mediate the assembly of proteins and lipids at the peroxisome membrane and the ER (13-16). In the budding yeast Saccharomyces cerevisiae, growth and division of peroxisomes are the dominant forms of peroxisome proliferation. However, contribution from the ER is an essential albeit poorly understood process. When peroxisomes are absent, cells form peroxisomes de novo from the ER. Presumably, interactions between proteins and lipids at the ER result in de novo biogenesis of nascent preperoxisomes that undergo a series of steps to ultimately form mature peroxisomes. Several peroxisomal membrane proteins (PMPs) insert into the ER dependent on components of the Sec61 complex, and the subsequent formation of preperoxisomes is dependent on the peroxins Pex3 and Pex19. Pex3 is an integral membrane protein that accumulates initially at an ER subdomain and is t...

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Section: Er-dependent Regulation Of Peroxisome Biogenesissupporting

confidence: 73%

Peroxins Pex30 and Pex29 Dynamically Associate with Reticulons to Regulate Peroxisome Biogenesis from the Endoplasmic Reticulum

Mast

Jamakhandi

Saleem

et al. 2016

Journal of Biological Chemistry

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“…No solid data exist on the preperoxisomal membrane carriers that would originate from the pER and ultimately sort to preexisting or nascent (daughter) peroxisomes in plants, but factors necessary for forming preperoxisomes are beginning to be identified in other organisms, such as Sec20p, Sec39p, and Dsl1p (Perry et al, 2009) as well as Sec16B (Yonekawa et al, 2011). In plants, small preperoxisomal membrane vesicles may bud from the ER and perhaps, prior to their fusion with preexisting peroxisomes, coalesce in a so-called ER-peroxisome intermediate compartment (ERPIC) (Mullen and Trelease, 2006;Trelease and Lingard, 2006), consistent with the proposed ERto-peroxisome vesicular transport pathways in certain yeasts and mammalian cells (reviewed in Titorenko and Rachubinski, 2009).…”

Section: Membrane Protein Trafficking From the Er To Peroxisomesmentioning

confidence: 99%

Plant Peroxisomes: Biogenesis and Function

et al. 2012

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Peroxisomes are eukaryotic organelles that are highly dynamic both in morphology and metabolism. Plant peroxisomes are involved in numerous processes, including primary and secondary metabolism, development, and responses to abiotic and biotic stresses. Considerable progress has been made in the identification of factors involved in peroxisomal biogenesis, revealing mechanisms that are both shared with and diverged from non-plant systems. Furthermore, recent advances have begun to reveal an unexpectedly large plant peroxisomal proteome and have increased our understanding of metabolic pathways in peroxisomes. Coordination of the biosynthesis, import, biochemical activity, and degradation of peroxisomal proteins allows for highly dynamic responses of peroxisomal metabolism to meet the needs of a plant. Knowledge gained from plant peroxisomal research will be instrumental to fully understanding the organelle's dynamic behavior and defining peroxisomal metabolic networks, thus allowing the development of molecular strategies for rational engineering of plant metabolism, biomass production, stress tolerance, and pathogen defense.

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“…Also, proteins of the endomembrane system have been implicated to serve a role in peroxisome biogenesis, such as Arf, coatomer (Lay et al, 2006), Sec20, and Sec39 (Perry et al, 2009). The molecular details of the role of these proteins in peroxisome biogenesis and proliferation need to be further elucidated.…”

Section: Introductionmentioning

confidence: 99%