Endoplasmic Reticulum Calcium Mediates<i>Drosophila</i>Wing Development

George, Laura Faith; Follmer, Mikaela; Fontenoy, Emily; Moran, Hannah R.; Brown, Jeremy; Ozekin, Yunus H.; Bates, Emily Anne

doi:10.1089/bioe.2022.0036

Cited by 2 publications

(1 citation statement)

References 101 publications

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“…Conversely, knock-out of Ca v 1.2 in the limb mesenchyme leads to shorter limbs due to impaired skeletal development [ 58 ]. In Drosophila wing discs, disruption of proteins that maintain ER Ca 2+ stores—such as the Serca2 Ca 2+ pump, the Orai Ca 2+ channel in the plasma membrane, the Best2 Cl - channel in the ER membrane, or Stim, a scaffold protein that colocalizes Orai and Best2—leads to mispatterned, stunted adult wings [ 59 ].…”

Section: Discussionmentioning

confidence: 99%

The scale of zebrafish pectoral fin buds is determined by intercellular K+ levels and consequent Ca2+-mediated signaling via retinoic acid regulation of Rcan2 and Kcnk5b

Jiang,

Zhao,

Sun

et al. 2024

PLoS Biol

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K+ channels regulate morphogens to scale adult fins, but little is known about what regulates the channels and how they control morphogen expression. Using the zebrafish pectoral fin bud as a model for early vertebrate fin/limb development, we found that K+ channels also scale this anatomical structure, and we determined how one K+-leak channel, Kcnk5b, integrates into its developmental program. From FLIM measurements of a FRET-based K+ sensor, we observed coordinated decreases in intracellular K+ levels during bud growth, and overexpression of K+-leak channels in vivo coordinately increased bud proportions. Retinoic acid, which can enhance fin/limb bud growth, decreased K+ in bud tissues and up-regulated regulator of calcineurin (rcan2). rcan2 overexpression increased bud growth and decreased K+, while CRISPR-Cas9 targeting of rcan2 decreased growth and increased K+. We observed similar results in the adult caudal fins. Moreover, CRISPR targeting of Kcnk5b revealed that Rcan2-mediated growth was dependent on the Kcnk5b. We also found that Kcnk5b enhanced depolarization in fin bud cells via Na+ channels and that this enhanced depolarization was required for Kcnk5b-enhanced growth. Lastly, Kcnk5b-induced shha transcription and bud growth required IP3R-mediated Ca2+ release and CaMKK activity. Thus, we provide a mechanism for how retinoic acid via rcan2 can regulate K+-channel activity to scale a vertebrate appendage via intercellular Ca2+ signaling.

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Section: Discussionmentioning

confidence: 99%

The scale of zebrafish pectoral fin buds is determined by intercellular K+ levels and consequent Ca2+-mediated signaling via retinoic acid regulation of Rcan2 and Kcnk5b

Jiang,

Zhao,

Sun

et al. 2024

PLoS Biol

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Transcriptomic Signature of Spatial Navigation in Brains of Desert Ants

Jaimes‐Nino,

Bar,

Subach

et al. 2024

Ecology and Evolution

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Navigation is crucial for central‐place foragers to locate food and return to the nest. Cataglyphis ants are renowned for their advanced navigation abilities, relying on landmark cues and path integration. This study aims to uncover the transcriptomic basis of exceptional spatial learning in the central nervous system of Cataglyphis niger. Ants navigated a maze with a food reward, and we examined expression changes linked to correct decisions in subsequent runs. Correct decisions correlated with expression changes in the optic lobes, but not the central brain, showing a downregulation of genes associated with sucrose response and Creb3l1. The latter gene is homologous to Drosophila crebA, which is essential for long‐term memory formation. To understand how ants use distance information during path integration, we analyzed expression shifts associated with the last distance traveled. We uncovered a transcriptomic footprint in the central brain, but not in the optic lobes, with genes enriched for energy consumption and neurological functions, including neuronal projection development, synaptic target inhibition, and recognition processes. This suggests that transcriptional activity in the central brain is necessary for estimating distance traveled, which is crucial for path integration. Our study supports the distinct roles of different brain parts for navigation in Cataglyphis ants.

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Endoplasmic Reticulum Calcium MediatesDrosophilaWing Development

Cited by 2 publications

References 101 publications

The scale of zebrafish pectoral fin buds is determined by intercellular K+ levels and consequent Ca2+-mediated signaling via retinoic acid regulation of Rcan2 and Kcnk5b

The scale of zebrafish pectoral fin buds is determined by intercellular K+ levels and consequent Ca2+-mediated signaling via retinoic acid regulation of Rcan2 and Kcnk5b

Transcriptomic Signature of Spatial Navigation in Brains of Desert Ants

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