Endoplasmic reticulum stress, chondrocyte apoptosis and oxidative stress in cartilage of broilers affected by spontaneous femoral head necrosis

Liu, Kangping; Fan, Rubin; Zhou, Zhenlei

doi:10.1016/j.psj.2021.101258

Cited by 10 publications

(4 citation statements)

References 55 publications

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“…As a bone disorder, FHN is characterized by periarticular bone disintegration and cartilage degeneration [ 22 ]. According to our previous study, FHN-affected broilers showed lipid metabolism disorders, increased lipid droplet formation, an imbalance in cartilage homeostasis, reduced extracellular matrix (ECM) formation, and simultaneous apoptosis [ 23 , 24 , 25 ]. Reduced osteogenic and chondrogenic differentiation of BMSCs in FHN-affected broilers and enhanced lipogenic differentiation were observed in the present study ( Figure 1 B).…”

Section: Discussionmentioning

confidence: 99%

“…Both sexes’ broilers (Ross 308) were acquired from a farm in Jiangsu Province. Femoral tissue from normal broilers and spontaneous FHN broilers was collected and stored in liquid nitrogen, as previously reported [ 23 , 24 ]. All animal protocols were approved by the Animal Protection and Use Committee of Nanjing Agricultural University (#NJAU-Poult-2019102205, approved on 22 October 2019).…”

Section: Methodsmentioning

confidence: 99%

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Notch-RBPJ Pathway for the Differentiation of Bone Marrow Mesenchymal Stem Cells in Femoral Head Necrosis

Liu

et al. 2023

IJMS

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Femoral head necrosis (FHN) is a common leg disease in broilers, resulting in economic losses in the poultry industry. The occurrence of FHN is closely related to the decrease in the number of bone marrow mesenchymal stem cells (BMSCs) and the change in differentiation direction. This study aimed to investigate the function of differentiation of BMSCs in the development of FHN. We isolated and cultured BMSCs from spontaneous FHN-affected broilers and normal broilers, assessed the ability of BMSCs into three lineages by multiple staining methods, and found that BMSCs isolated from FHN-affected broilers demonstrated enhanced lipogenic differentiation, activated Notch-RBPJ signaling pathway, and diminished osteogenic and chondrogenic differentiation. The treatment of BMSCs with methylprednisolone (MP) revealed a significant decrease in the expressions of Runx2, BMP2, Col2a1 and Aggrecan, while the expressions of p-Notch1/Notch1, Notch2 and RBPJ were increased significantly. Jagged-1 (JAG-1, Notch activator)/DAPT (γ-secretase inhibitor) could promote/inhibit the osteogenic or chondrogenic ability of MP-treated BMSCs, respectively, whereas the differentiation ability of BMSCs was restored after transfection with si-RBPJ. The above results suggest that the Notch-RBPJ pathway plays important role in FHN progression by modulating the osteogenic and chondrogenic differentiation of BMSCs.

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Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Notch-RBPJ Pathway for the Differentiation of Bone Marrow Mesenchymal Stem Cells in Femoral Head Necrosis

Liu

et al. 2023

IJMS

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“…In view of this, the correlation between VEGF alterations and adipokines in synovial fluid and serum during OA is very interesting (41). As adipokines and VEGF exerts direct and indirect effects on bone metabolism and blood vessels, they might also be implicated in the development of several bone diseases including osteoporosis, osteochondrosis and osteochondritis dissecans (OC/OCD) like lesions, avascular necrosis of the femoral head and canine hypertrophic osteodystrophy (Figure 1) (42)(43)(44)(45). In addition to clinical and animal model studies, in vitro studies using mechanical stress loading of osteoblast and chondrocytes and assessing their response to various adipokines might be instrumental in unraveling the complex interactions (13).…”

Section: The Role Of Adipokinesmentioning

confidence: 99%

“…In addition to clinical and animal model studies, in vitro studies using mechanical stress loading of osteoblast and chondrocytes and assessing their response to various adipokines might be instrumental in unraveling the complex interactions (13). This also allows for assessment of intracellular metabolism including mitochondrial function and endoplasmic reticulum stress (45)(46)(47)(48)(49). It is postulated that adipokines could play a causative role in the development of several joint diseases leading to OA.…”

Section: The Role Of Adipokinesmentioning

confidence: 99%

Osteoarthritis, adipokines and the translational research potential in small animal patients

Theyse,

Mazur

2024

Front. Vet. Sci.

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Osteoartritis (OA) is a debilitating disease affecting both humans and animals. In the early stages, OA is characterized by damage to the extracellular matrix (ECM) and apoptosis and depletion of chondrocytes. OA progression is characterized by hyaline cartilage loss, chondrophyte and osteophyte formation, thickening of the joint capsule and function loss in the later stages. As the regenerative potential of cartilage is very limited and osteoarthritic changes are irreversible, prevention of OA, modulation of existing osteoarthritic joint inflammation, reducing joint pain and supporting joint function are the only options. Progression of OA and pain may necessitate surgical intervention with joint replacement or arthrodesis as end-stage procedures. In human medicine, the role of adipokines in the development and progression of OA has received increasing interest. At present, the known adipokines include leptin, adiponectin, visfatin, resistin, progranulin, chemerin, lipocalin-2, vaspin, omentin-1 and nesfatin. Adipokines have been demonstrated to play a pivotal role in joint homeostasis by modulating anabolic and catabolic balance, autophagy, apoptosis and inflammatory responses. In small animals, in terms of dogs and cats, naturally occurring OA has been clearly demonstrated as a clinical problem. Similar to humans, the etiology of OA is multifactorial and has not been fully elucidated. Humans, dogs and cats share many joint related degenerative diseases leading to OA. In this review, joint homeostasis, OA, adipokines and the most common joint diseases in small animals leading to naturally occurring OA and their relation with adipokines are discussed. The purpose of this review is highlighting the translational potential of OA and adipokines research in small animal patients.

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Bioinformatics proved the existence of potential hub genes activating autophagy to participate in cartilage degeneration in osteonecrosis of the femoral head

Zhu,

Wang,

Liu

2024

J Mol Histol

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Endoplasmic reticulum stress, chondrocyte apoptosis and oxidative stress in cartilage of broilers affected by spontaneous femoral head necrosis

Cited by 10 publications

References 55 publications

Notch-RBPJ Pathway for the Differentiation of Bone Marrow Mesenchymal Stem Cells in Femoral Head Necrosis

Notch-RBPJ Pathway for the Differentiation of Bone Marrow Mesenchymal Stem Cells in Femoral Head Necrosis

Osteoarthritis, adipokines and the translational research potential in small animal patients

Bioinformatics proved the existence of potential hub genes activating autophagy to participate in cartilage degeneration in osteonecrosis of the femoral head

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