Endoplasmic Reticulum Stress Response of Bombyx Mori Calreticulin

Goo, Tae Won; Park, Soo-Jung; Jin, Byung Rae; Yun, Eun Young; Kim, Iksoo; Nho, Si Kab; Kang, Seok-Woo; Kwon, O‐Yu

doi:10.1007/s11033-004-5908-7

Cited by 10 publications

(15 citation statements)

References 32 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Further more, calreticulin located in the endoplasmic reticulum, has been implicated in many diverse functions, including: regulation of intracellular Ca 2+ homeostasis, chaperone activity, steroid-mediated gene regulation, and cell adhesion [58]. The highest level of mRNA expression of calreticulin was exhibited in the fat body of Bombyx mori [59]. Our result showed that calreticulin was identified during the initial embryogenesis both in D and ND samples, which means that calreticulin is essential during embryonic development.…”

Section: Resultsmentioning

confidence: 53%

Shotgun Proteomic Analysis on the Diapause and Non-Diapause Eggs of Domesticated Silkworm Bombyx mori

et al. 2013

View full text Add to dashboard Cite

To clarify the molecular mechanisms of silkworm diapause, it is necessary to investigate the molecular basis at protein level. Here, the spectra of peptides digested from silkworm diapause and non-diapause eggs were obtained from liquid chromatography tandem mass spectrometry (LC-MS/MS) and were analyzed by bioinformatics methods. A total of 501 and 562 proteins were identified from the diapause and non-diapause eggs respectively, of which 309 proteins were shared commonly. Among these common-expressed proteins, three main storage proteins (vitellogenin precursor, egg-specific protein and low molecular lipoprotein 30 K precursor), nine heat shock proteins (HSP19.9, 20.1, 20.4, 20.8, 21.4, 23.7, 70, 90-kDa heat shock protein and heat shock cognate protein), 37 metabolic enzymes, 22 ribosomal proteins were identified. There were 192 and 253 unique proteins identified in the diapause and non-diapause eggs respectively, of which 24 and 48 had functional annotations, these unique proteins indicated that the metabolism, translation of the mRNA and synthesis of proteins were potentially more highly represented in the non-dipause eggs than that in the diapause eggs. The relative mRNA levels of four identified proteins in the two kinds of eggs were also compared using quantitative reverse transcription PCR (qRT-PCR) and showed some inconsistencies with protein expression. GO signatures of 486 out of the 502 and 545 out of the 562 proteins identified in the diapause and non-diapause eggs respectively were available. In addition, Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis showed the Metabolism, Translation and Transcription pathway were potentially more active in the non-dipause eggs at this stage.

show abstract

Section: Resultsmentioning

confidence: 53%

Shotgun Proteomic Analysis on the Diapause and Non-Diapause Eggs of Domesticated Silkworm Bombyx mori

et al. 2013

View full text Add to dashboard Cite

show abstract

“…Recently, the calreticulin gene ( BmCRT ) was cloned and characterized from B . mori (Goo et al . 2005).…”

Section: Resultsmentioning

confidence: 99%

“…As shown in Figure 4, the transcripts of PxCRT are almost undetectable in the fat body, are expressed at higher levels in both midgut and Malpighian tubules and peak in the silk glands. Recently, the calreticulin gene (BmCRT) was cloned and characterized from B. mori (Goo et al 2005). Northern blot analysis shows that BmCRT is mainly expressed in the fat body, not the silk glands.…”

Section: Overexpression Of Pxcrt Transcripts In Silk Glandmentioning

confidence: 99%

Molecular cloning and expression profiles of calreticulin gene from the diamondback moth, Plutella xylostella

Kim

Noh

et al. 2010

Entomological Research

Self Cite

View full text Add to dashboard Cite

Calreticulin (CRT) plays pivotal roles in Ca2+ homeostasis, molecular chaperoning, infection, inflammation and innate immunity. In an attempt to study the involvement of CRT in innate immunity, the full‐length cDNA of calreticulin (PxCRT) was cloned from the diamondback moth, Plutella xylostella. It consists of 1674 bp (excluding poly‐A tail) with a longest open reading frame (ORF) of 1197 bp encoding 398 amino acids. In silico analysis of PxCRT ORF reveals that it has various repeat motifs and endoplasmic reticulum retention signal found in all the calreticulin proteins. As expected, high amino acid sequence identities were found from other CRTs identified from Bombyx mori (87%), Galleria mellonella (87%), Apis mellifera (74%), Anopheles gambiae (74%), Tribolium castaneum (73%), Culex quinquefasciatus (73%), Rhodnius prolixus (72%), Nasonia vitripennis (71%), Drosophila melanogaster (71%) and Haemaphysalis qinghaiensis (68%). During development, P. xylostella expressed PxCRT predominantly in the pupal stage. In addition, spatial expression pattern analysis indicates that PxCRT was highly expressed in the silk gland. PxCRT mRNA, furthermore, was strongly induced 3 to 6 h after laminarin treatment, suggesting that PxCRT appears to be involved in immune responses and also plays an important role in the silk gland.

show abstract

“…The up-regulation of CRT expression level also might be attributed to the chaperone function and Ca 2? homeostasis (Goo et al 2005;Luana et al 2007). ROSinduced lipid peroxidation also leads to the loss of ability of endoplasmic reticular membranes (Lehotsky et al 2002), and ultimately to trigger the production of Ca 2?…”

Section: Responses Of Stress Protein Gene Expressionmentioning

confidence: 99%

Multilevel ecotoxicity assessment of polycyclic musk in the earthworm Eisenia fetida using traditional and molecular endpoints

et al. 2011

View full text Add to dashboard Cite

The ecotoxicity assessment of galaxolide (HHCB) and tonalide (AHTN) was investigated in the earthworm Eisenia fetida using traditional and novel molecular endpoints. The median lethal concentration (LC(50)) for 7-day and 14-day exposures was 573.2 and 436.3 μg g(-1) for AHTN, and 489.0 and 392.4 μg g(-1) for HHCB, respectively. There was no observed significant effect on the growth rate of E. fetida after a 28-day exposure except that at the highest concentration (100 μg g(-1)) of AHTN and HHCB, whereas a significant decrease of cocoon production was found in earthworms exposed to 50 and 100 μg g(-1). To assess molecular-level effect, the expression of encoding antioxidant enzymes and stress protein genes were investigated upon sublethal exposures using the quantitative real time PCR assay. The expression level of SOD, CAT and calreticulin genes was up-regulated significantly, while the level of annetocin (ANN) and Hsp70 gene expression was down-regulated in E. fetida. Importantly, the level of ANN expression had a significant positive correlation with the reproduction rate of earthworms. Furthermore, the lowest observed effect concentration (LOECs) of ANN expression level was 3 μg g(-1) for AHTN and 10 μg g(-1) for HHCB, suggesting that ANN gene expression can serve as a more sensitive indicator of exposure to AHTN and HHCB than traditional endpoints such as cocoon production. The transcriptional responses of these genes may provide early warning molecular biomarkers for identifying contaminant exposure, and the data obtained from this study will contribute to better understand the toxicological effect of AHTN and HHCB.

show abstract

Endoplasmic Reticulum Stress Response of Bombyx Mori Calreticulin

Cited by 10 publications

References 32 publications

Shotgun Proteomic Analysis on the Diapause and Non-Diapause Eggs of Domesticated Silkworm Bombyx mori

Shotgun Proteomic Analysis on the Diapause and Non-Diapause Eggs of Domesticated Silkworm Bombyx mori

Molecular cloning and expression profiles of calreticulin gene from the diamondback moth, Plutella xylostella

Multilevel ecotoxicity assessment of polycyclic musk in the earthworm Eisenia fetida using traditional and molecular endpoints

Contact Info

Product

Resources

About