Endoplasmic reticulum stress triggers ROS signalling, changes the redox state, and regulates the antioxidant defence of Arabidopsis thaliana

Özgür, Rengin; Türkan, İsmail; Uzilday, Barış; Sekmen, A. Hediye

doi:10.1093/jxb/eru034

Cited by 156 publications

(119 citation statements)

References 68 publications

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“…Accumulation of unfolded proteins (which can be induced by the ER stress agent tunicamycin) causes excessive production of H 2 O 2 in the ER. This, in turn, can trigger ROS signaling via NOX in the plasma membrane and can alter the glutathione redox state (Ozgur et al, 2014). Thus, the hemifused membrane model and the coordinated movement of stromules and ER tubules may enable the ER to send out redox signals to chloroplasts via H 2 O 2 , especially under high temperatures, where protein folding is significantly affected and the unfolded protein response is induced (Liu and Howell, 2010).…”

Section: Reverse Coupling Of Redox Signaling To the Chloroplastmentioning

confidence: 99%

Redox- and Reactive Oxygen Species-Dependent Signaling into and out of the Photosynthesizing Chloroplast

Türkan²,

2016

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Photosynthesis is a high-rate redox metabolic process that is subjected to rapid changes in input parameters, particularly light. Rapid transients of photon capture, electron fluxes, and redox potentials during photosynthesis cause reactive oxygen species (ROS) to be released, including singlet oxygen, superoxide anion radicals, and hydrogen peroxide. Thus, the photosynthesizing chloroplast functions as a conditional source of important redox and ROS information, which is exploited to tune processes both inside the chloroplast and, following retrograde release or processing, in the cytosol and nucleus. Analyses of mutants and comparative transcriptome profiling have led to the identification of these processes and associated players and have allowed the specificity and generality of response patterns to be defined. The release of ROS and oxidation products, envelope permeabilization (for larger molecules), and metabolic interference with mitochondria and peroxisomes produce an intricate ROS and redox signature, which controls acclimation processes. This photosynthesis-related ROS and redox information feeds into various pathways (e.g. the mitogen-activated protein kinase and OXI1 signaling pathways) and controls processes such as gene expression and translation.

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Section: Reverse Coupling Of Redox Signaling To the Chloroplastmentioning

confidence: 99%

Redox- and Reactive Oxygen Species-Dependent Signaling into and out of the Photosynthesizing Chloroplast

Türkan²,

2016

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“…SOD activity was detected by Ozgur et al (2014) ried out. Total POX (EC 1.11.1.7) activity was based on the method described by Ozgur et al (2014). The increase in the absorbance at 465 nm was followed for 3 min.…”

Section: Enzyme Extraction and Determination Of Isozyme And/or Enzymementioning

confidence: 99%

“…The level of lipid peroxidation was determined by thiobarbituric acid reactive substances (TBARS) according to Ozgur et al (2014) with minor revisions.…”

Section: Determination Of Lipid Peroxidation Levelsmentioning

confidence: 99%

Improvement of cold stress resistance via free radical scavenging ability and promoted water status and photosynthetic capacity of gallic acid in soybean leaves

Yıldıztugay

Özfidan-Konakçi

Küçüködük

2017

J. Soil Sci. Plant Nutr.

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Plant polyphenols exhibit a wide variety of biological activities such as antimutagenicity, anticarcinogenicity and antioxidative activity. There is no report whether gallic acid (GLA), a naturally occurring plant phenol, is able to activate the plant defense system under cold stress. For this purpose, after soybean (Glycine max) was hydroponically grown for 3 weeks, seedlings were treated with gallic acid (GLA; 1 and 2 mM) and cold stress (5 o C and 10 o C) and GLA and stress combination for 72 h. The inhibition in growth, water content (RWC), osmotic potential (Ψп) and photosynthetic activity observed under stress and was more at the lowest temperature. Stress also elicited the accumulation of proline (Pro) only at 5 o C. While the capacity to maintain high growth, RWC, Ψп and photosynthetic efficiency was observed in GLA-treated plants under stress, Pro accumulation could not achieve with GLA plus stress. Any increase in total activities of superoxide dismutase (SOD) and catalase (CAT) induced by stress treatments determined. The lower cold stress caused an increase in the activities of ascorbate peroxidase (APX), glutathione reductase (GR) and NADPH oxidase (NOX). GLA treatment under stress (especially at 5 o C) could supply the increased activities of SOD, CAT, APX and GR. Also, exogenous GLA application to stress-treated plants increased the enzyme activities in ascorbate-glutathione cycle such as monodehydroascorbate reductase (MDHAR) and dehydroascorbate reductase (DHAR) and, contents of ascorbate (AsA) and glutathione. After GLA application under stress, it is observed reduction in hydrogen peroxide (H 2 O 2 ) and the levels of lipid peroxidation (TBARS), and induction of hydroxyl radical (OH • ) scavenging. Our results suggest that GLA is a potent inducer for induction of the scavenging activity of radicals as well as effectively usage of water status and photosynthetic capacity.

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“…ERO1 uses molecular oxygen as the final electron acceptor and hence, forms one molecule of hydrogen peroxide for every disulfide that it introduces (Tu and Weissman, 2004). Several studies have established an association between ER stress and ROS generation (Malhotra and Kaufman, 2007;Rutkowski and Kaufman, 2007;Ozgur et al, 2014), and the molecular mechanisms by which ROS are produced during UPR have been thoroughly reviewed (Santos et al, 2009).…”

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confidence: 99%

Oxidative Stress Contributes to Autophagy Induction in Response to Endoplasmic Reticulum Stress in Chlamydomonas reinhardtii

et al. 2014

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The accumulation of unfolded/misfolded proteins in the endoplasmic reticulum (ER) results in the activation of stress responses, such as the unfolded protein response or the catabolic process of autophagy to ultimately recover cellular homeostasis. ER stress also promotes the production of reactive oxygen species, which play an important role in autophagy regulation. However, it remains unknown whether reactive oxygen species are involved in ER stress-induced autophagy. In this study, we provide evidence connecting redox imbalance caused by ER stress and autophagy activation in the model unicellular green alga Chlamydomonas reinhardtii. Treatment of C. reinhardtii cells with the ER stressors tunicamycin or dithiothreitol resulted in up-regulation of the expression of genes encoding ER resident endoplasmic reticulum oxidoreductin1 oxidoreductase and protein disulfide isomerases. ER stress also triggered autophagy in C. reinhardtii based on the protein abundance, lipidation, cellular distribution, and mRNA levels of the autophagy marker ATG8. Moreover, increases in the oxidation of the glutathione pool and the expression of oxidative stressrelated genes were detected in tunicamycin-treated cells. Our results revealed that the antioxidant glutathione partially suppressed ER stress-induced autophagy and decreased the toxicity of tunicamycin, suggesting that oxidative stress participates in the control of autophagy in response to ER stress in C. reinhardtii In close agreement, we also found that autophagy activation by tunicamycin was more pronounced in the C. reinhardtii sor1 mutant, which shows increased expression of oxidative stress-related genes.

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Endoplasmic reticulum stress triggers ROS signalling, changes the redox state, and regulates the antioxidant defence of Arabidopsis thaliana

Abstract: SummaryEndoplasmic reticulum stress, which is induced by tunicamycin, triggers reactive oxygen species signalling via NADPH oxidase activity and also regulates the antioxidant defence system in Arabidopsis thaliana.

Cited by 156 publications

References 68 publications

Redox- and Reactive Oxygen Species-Dependent Signaling into and out of the Photosynthesizing Chloroplast

Redox- and Reactive Oxygen Species-Dependent Signaling into and out of the Photosynthesizing Chloroplast

Improvement of cold stress resistance via free radical scavenging ability and promoted water status and photosynthetic capacity of gallic acid in soybean leaves

Oxidative Stress Contributes to Autophagy Induction in Response to Endoplasmic Reticulum Stress in Chlamydomonas reinhardtii

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