Endopolygalacturonase production from Kluyveromyces marxianus. I. Resolution, purification, and partial characterisation of the enzyme

Purification and characterization of acidic endo-polygalacturonase encoded by the PGL1-1 gene from Saccharomyces cerevisiae

“…Glycosylation has been described for the endoPG from Kluyveromyces marxianus [34], Aspergillus [35] and S. sclerotiorum [30]. The treatment of Pgl1P with an endo-L-Nacetyl glucosaminidase H induced a decrease of the M r from 42 to 37 kDa as shown by SDS-PAGE (Fig.…”

Low temperature active pectinases production by Saccharomyces cerevisiae isolate and their characterization

“…Pectinases have been reported from various yeast species of Saccharomyces, Kluyveromyces, Cryptococcus, Rhodotorola, Aureobasidium pullulans and Candida (Luh and Phaf 1951;Vaugn et al, 1969;Winborne and Richard, 1978;Lim et al,1980;Federici, 1985;Barnby et al, 1990;Blanco et al, 1994;Moyo et al, 2003;da Silva et al, 2005;Merin et al, 2011), especially polygalacturonases (Blanco et al, 1994;Birgisson et al, 2003).Yeasts present an alternative source for the large-scale production of commercial enzymes (Biely 1994;Blanco et al, 1994;Braga et al, 1998;Jia and Wheals, 2000). They were proved to be advantageous over moulds with regard to the production of pectinases, because of their unicellular nature, simple growth and nonrequirement of inducer in growth medium.…”

Cloning, sequence analysis and overexpression of aSaccharomyces cerevisiae endopolygalacturonase-encoding gene (PGL1)

“…Although S. cerevisiae is frequently used in the food industry, a few studies have concerned their pectolytic abilities (Luh and Phaff, 1951;Barnby et al, 1990;McKay, 1990;Gainvors et al, 1994a, b;Gainvors and Belarbi, 1995;Blanco et al, 1997). In an attempt to develop S. cerevisiae strains able to degrade pectins and glucans during alcoholic fermentations, Pretorius (1992, 1993a,b) and Van Rensburg et al (1994) constructed recombinant S. cerevisiae strains expressing the Erwinia chrysanthemi pectate lyase gene, the Erwinia carotovora polygalacturonase gene and the Butyrivibrio fibrisolvens endo--1,4-glucanase in wine and distillers' yeast.…”