Endorphins exert opiate‐like action on neuroblastoma X glioma hybrid cells

Brandt, Michael R.; Buchen, Claudia; Hamprecht, Bernd

doi:10.1016/0014-5793(77)80451-1

Cited by 17 publications

(2 citation statements)

References 25 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Since, as discussed above, it seems unlikely that histamine and serotonin share receptor sites, we must postulate that serotonin, via its receptor, exerts an inhibitory influence on histamine-dependent cyclic AMP stimulation. Examples of such inhibitory pathways for two different receptors have been reported in studies on neuroblastoma-glioma hybrids, where a-adrenergic agonists (Sabol and Nirenberg, 1979) and opiates (Sharma et al, 1977;Brandt et al, 1977), acting through their respective receptors, inhibit prostaglandin El-stimulated and basal adenylate cyclase activities. In the case of opiates, this appears to be done by stimulating GTP hydrolysis (Koski and Klee, 1981).…”

Section: Discussionmentioning

confidence: 96%

Hormones and Neurotransmitters Control Cyclic AMP Metabolism in Choroid Plexus Epithelial Cells

Crook

Farber

Prusiner

1984

Journal of Neurochemistry

View full text Add to dashboard Cite

The choroid plexus is a major site of CSF production. When primary cultures of bovine choroid plexus epithelial cells were exposed to 1 micrograms/ml cholera toxin, a 50-fold increase of intracellular cyclic AMP was found 1 h later. Exposure of cells to 10(-5) M isoproterenol, 10(-4) M prostaglandin E1, 10(-5) M histamine, and 10(-5) M serotonin caused increases of intracellular cyclic concentrations of 100-, 50-, 20-, and 4-fold, respectively. From 5 to 15 min were required for these maximal responses to occur. Many other molecules including prolactin, vasopressin, and corticotropin did not alter cellular cyclic AMP levels. The accumulation of cyclic AMP could be inhibited by specific antagonists: propranolol inhibited the isoproterenol-mediated stimulation while diphenhydramine and metiamide inhibited the histamine response. In addition, diphenhydramine inhibited serotonin-dependent cyclic AMP accumulation. Combinations of isoproterenol, prostaglandin E1, histamine, and serotonin elicited additive responses as measured by cyclic AMP accumulation with one exception, i.e., serotonin inhibited the histamine response. Our findings suggest that distinct receptor sites on choroid plexus epithelia exist for isoproterenol, prostaglandin E1, and histamine. Efflux of cyclic AMP into the extracellular medium was found to be a function of the intracellular cyclic AMP levels over a wide range of concentrations. Our studies provide direct evidence for hormonal regulation of cyclic AMP metabolism in epithelial cells of the choroid plexus.

show abstract

Section: Discussionmentioning

confidence: 96%

Hormones and Neurotransmitters Control Cyclic AMP Metabolism in Choroid Plexus Epithelial Cells

Crook

Farber

Prusiner

1984

Journal of Neurochemistry

View full text Add to dashboard Cite

show abstract

“…The neuroblastoma-glioma hybrid cell line NG108-15 has high levels of opiate receptors (6) that are both functionally linked to adenylate cyclase (7,8) and homogeneous with respect to ligand preference (9,10), showing high affinity for enkephalin-like compounds. The ability of 3endorphin to act on the adenylate cyclase system in NG108-15 cells has been demonstrated (11).…”

mentioning

confidence: 99%

beta-Endorphin: characteristics of binding sites in a neuroblastoma--glioma hybrid cell.

Hammonds¹,

Ferrara²,

Li³

1981

Proc. Natl. Acad. Sci. U.S.A.

View full text Add to dashboard Cite

Specific binding of human beta-endorphin to NG108-15 cells is described; human beta-[Tyr27-3H2] endorphin was used as the ligand. The binding is time dependent and saturable; Kd = 0.3 nM and ka = 1.8 x 10(8) M-1 min-1. Under the conditions optimal for beta-endorphin binding, leucine-enkephalin has one-fourth to one-third as many binding sites as beta-endorphin and its affinity is 7--10% that of beta-endorphin. Monovalent and divalent cations potently inhibit binding. Trypsin, phospholipase A, and N-ethylmaleimide reduce the ability of NG108-15 cells to bind beta-endorphin. beta-Endorphin analogs are able to fully inhibit the binding of beta-[Tyr27-3H2]endorphin, although enkephalins, morphine, and naloxone inhibit only 50--80%.

show abstract

Opioid Peptides as Modulators of Cyclic AMP Levels

Klee

1979

Modulators, Mediators, and Specifiers in Brain Function

View full text Add to dashboard Cite

Endorphins exert opiate‐like action on neuroblastoma X glioma hybrid cells

Cited by 17 publications

References 25 publications

Hormones and Neurotransmitters Control Cyclic AMP Metabolism in Choroid Plexus Epithelial Cells

Hormones and Neurotransmitters Control Cyclic AMP Metabolism in Choroid Plexus Epithelial Cells

beta-Endorphin: characteristics of binding sites in a neuroblastoma--glioma hybrid cell.

Opioid Peptides as Modulators of Cyclic AMP Levels

Contact Info

Product

Resources

About