An isometer, a highly compliant spring-scale device for measuring suture displacement, has been used intraoperatively by surgeons to select the optimal placement of the femoral tunnel for an anterior cruciate ligament graft. The isometer measures the displacement of a suture centered in a tibial tunnel and attached to an intraarticular location on the femur before the femoral tunnel is drilled. Because the placement of the femoral tunnel strongly impacts the tensile behavior of an anterior cruciate ligament graft and because surgeons have used the amount of suture displacement to guide the placement of the femoral tunnel, the objective of this study was to determine the ability of an isometer to predict graft tension. In 14 patients undergoing reconstructive surgery of the anterior cruciate ligament, an isometer was used to measure suture displacement during passive knee motion for a provisional femoral tunnel location. An electrogoniometer recorded the flexion angle of the knee. The femoral tunnel was drilled. A double-looped semitendinosus and gracilis autograft was inserted around a post in the femoral tunnel, and the tension in the four limbs of the graft exiting the tibial tunnel was measured during passive knee motion. Graft-tension versus knee-flexion-angle curves revealed that each knee exhibited one of two distinct curve shapes: L-shaped, characterized by the maximum tension occurring at full extension and a nearly flat profile from 35 to 90 degrees of flexion, or U-shaped, with elevated tensions at 80-90 degrees of flexion (p < 0.001) reaching at least half of the tension in full extension. Because the shapes of the suture-displacement versus flexion-angle curves were more consistently L-shaped, the intraoperative measurement of suture displacement was not a useful predictor of either the increase in tension in the graft with flexion or the maximum tension in the graft.