Endoscopic multiple biopsy and rapid diagnosis by in situ fixation and histopathologic processing

Abstract

Single-pass multiple biopsy reduces biopsy time with less specimen damage, work, workplace risk, and soiling. Diagnostic quality is equal to forceps biopsy with better epithelial preservation, although 26% smaller. In pathology, in situ processing and microtomy reduce work and workplace risk. Grossing and manual orientation are unnecessary. Rapid diagnosis by frozen section and microwave or paraffin processing are facilitated. Multiple biopsy speeds diagnosis and improves productivity in endoscopic biopsy and histopathologic processing.

Comparison of standard single‐bite with multiple‐bite biopsy forceps for collection of gastrointestinal biopsies in dogs: a prospective study

“…The smaller size is likely due to the restricted jaw aperture (measured at 2.4 mm) preventing collection of larger samples. A similar observation was made by Zimmon and others 9 in the process of designing and piloting the Curlew, a new Multibite forceps. In their study, the authors objectively assessed the size of the biopsies by measuring them with an ocular reticle.…”

“…2 Similar scoring systems integrating depth, crush artefacts and diagnostic quality or adequacy are also used in human medicine to assess and compare endoscopic biopsy forceps. [9][10][11] Histological assessment of inlammation can vary between pathologists and despite the use of scoring systems or pictorial templates, agreement between pathologists remains poor. 13 14 However, biopsies in this study were scored by a single pathologist, which minimised variability.…”

“…This contradicts the results of another study, which found that each biopsy obtained with a single bite forceps added 61 seconds to the procedure in comparison with the MB. 9 One explanation for this discrepancy was the overall diiculty to use the MB forceps. All clinicians involved reported issues with the collection of the specimens; on several occasions, samples already taken were seen to be dislodged from the forceps cup while attempting to obtain additional ones (data not shown).…”

“…In order to achieve that with conventional forceps, the endoscopist has to insert and retrieve the forceps at least as many times as the number of biopsies required, increasing endoscopic procedure time and anaesthesia duration for the patient. 9 Multiple-bite biopsy forceps are designed to collect and store biopsies in their specially designed cups in order to reduce the number of passes through the operating channel. The Multibite forceps (Microvasive Endoscopy, Boston Scientiic, Natick, Massachussets, USA) has been evaluated in human patients undergoing upper and lower gastrointestinal endoscopy for routine clinical investigations.…”

A Position-Adjustable Multi-Point Synchronizing Biopsy Tool for Intratumor Heterogeneity: A Proof-of-Principle Study

“…Thus, biopsy needle with multiple-sample within one insertion is a preferable choice for obtaining information of intratumor heterogeneity. A rounded tip endoscopic tool is designed for Multiple Specimens with a Single jawed Spoon (MS-SS) [15], and the biopsies are collected up to 25 and stored in a plastic chamber inside the removable metal tip. A biopsy needle instrument is designed to capture a plurality of discrete specimens without being withdrawn from pathological tissue [16].…”