Endoscopic Raman spectroscopy enables objective diagnosis of dysplasia in Barrett's esophagus

Almond, L. Max; Hutchings, Joanne; Lloyd, Gavin R.; Barr, Hugh; Shepherd, Neil A.; Day, John C C; Stevens, Oliver A. C.; Sanders, Scott; Wadley, M; Stone, Nick; Kendall, Catherine

doi:10.1016/j.gie.2013.05.028

Cited by 107 publications

(69 citation statements)

References 21 publications

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“…98 In an ex vivo study of 673 samples of BE (acquired from 62 patients), endoscopic Raman spectroscopy demonstrated a sensitivity of 86% and a specificity of 88% for distinguishing advanced neoplasia (HGD/adenocarcinoma) from LGD, nondysplastic BE and normal squamous oesophagus tissue. 99 BE tissues with various conditions show different optical physiological characteristics which are valuable for discriminant diagnosis. Bergholt et al 100 utilized confocal Raman spectroscopy to detect BE in vivo and recorded a series of peaks that reflected expression levels of different substances (proteins, lipids and porphyrins) in columnarlined epithelium (CLE) and nondysplastic BE and HGD BE, by which they obtained a sensitivity of 87% (67/77) and specificity of 84.7% (610/720) for detecting HGD in BE and AUROCs of 0.88, 0.84 and 0.90 for the discriminations of CLE versus nondysplastic and HGD BE, nondysplastic BE versus CLE and HGD BE and HGD BE versus CLE and nondysplastic BE, and the suspicious BE according to this technique was also confirmed by subsequent biopsy and histopathology.…”

Section: Oesophageal Cancermentioning

confidence: 99%

Biomedical optical spectroscopy for the early diagnosis of gastrointestinal neoplasms

2017

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Gastrointestinal cancer is a leading contributor to cancer-related morbidity and mortality worldwide. Early diagnosis currently plays a key role in the prognosis of patients with gastrointestinal cancer. Despite the advances in endoscopy over the last decades, missing lesions, undersampling and incorrect sampling in biopsies, as well as invasion still result in a poor diagnostic rate of early gastrointestinal cancers. Accordingly, there is a pressing need to develop noninvasive methods for the early detection of gastrointestinal cancers. Biomedical optical spectroscopy, including infrared spectroscopy, Raman spectroscopy, diffuse scattering spectroscopy and autofluorescence, is capable of providing structural and chemical information about biological specimens with the advantages of non-destruction, non-invasion and reagent-free and waste-free analysis and has thus been widely investigated for the diagnosis of oesophageal, gastric and colorectal cancers. This review will introduce the advances of biomedical optical spectroscopy techniques, highlight their applications for the early detection of gastrointestinal cancers and discuss their limitations.

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Section: Oesophageal Cancermentioning

confidence: 99%

Biomedical optical spectroscopy for the early diagnosis of gastrointestinal neoplasms

2017

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“…18,20 Other modalities, such as fiber-based optical coherence tomography and Raman spectroscopy, could also be used in conjunction to accomplish imaging at multiple resolutions. 33,34 …”

Section: Discussionmentioning

confidence: 99%

In vivowhite light and contrast-enhanced vital-dye fluorescence imaging of Barrett’s-related neoplasia in a single-endoscopic insertion

et al. 2016

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Abstract. A modular video endoscope is developed to enable both white light imaging (WLI) and vital-dye fluorescence imaging (VFI) in a single-endoscopic insertion for the early detection of cancer in Barrett's esophagus (BE). We demonstrate that VFI can be achieved in conjunction with white light endoscopy, where appropriate white balance is used to correct for the presence of the emission filter. In VFI mode, a contrast enhancement feature is implemented in real time to further highlight glandular patterns in BE and related malignancies without introducing artifacts. In a pilot study, we demonstrate accurate correlation of images in two widefield modalities, with representative images showing the disruption and effacement of glandular architecture associated with cancer development in BE. VFI images of these alterations exhibit enhanced contrast when compared to WLI. Results suggest that the usefulness of VFI in the detection of BE-related neoplasia should be further evaluated in future in vivo studies. © The Authors. Published by SPIE under a Creative Commons Attribution 3.0 Unported License. Distribution or reproduction of this work in whole or in part requires full attribution of the original publication, including its DOI.

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“…In other studies, fiber-optic endoscopic probes have been used in conjunction with Raman spectroscopy for the analysis of esophageal and gastric cancer in vivo and ex vivo [36, 37]. This is a promising tool for intraoperative diagnosis during endoscopic resection of esophageal intramucosal cancer.…”

Section: Discussionmentioning

confidence: 99%

Assessment of Tumor Cells in a Mouse Model of Diffuse Infiltrative Glioma by Raman Spectroscopy

Tanahashi

Natsume

Ohka

et al. 2014

BioMed Research International

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Glioma of infiltrative nature is challenging for surgeons to achieve tumor-specific and maximal resection. Raman spectroscopy provides structural information on the targeted materials as vibrational shifts. We utilized Raman spectroscopy to distinguish invasive tumors from normal tissues. Spectra obtained from replication-competent avian sarcoma-(RCAS-) based infiltrative glioma cells and glioma tissues (resembling low-grade human glioma) were compared with those obtained from normal mouse astrocytes and normal tissues. In cell analysis, the spectra at 950–1000, 1030, 1050–1100, 1120–1130, 1120–1200, 1200–1300, 1300–1350, and 1450 cm−1 were significantly higher in infiltrative glioma cells than in normal astrocytes. In brain tissue analysis, the spectra at 1030, 1050–1100, and 1200–1300 cm−1 were significantly higher in infiltrative glioma tissues than in normal brain tissues. These spectra reflect the structures of proteins, lipids, and DNA content. The sensitivity and specificity to predict glioma cells by distinguishing normal cells were 98.3% and 75.0%, respectively. Principal component analysis elucidated the significance of spectral difference between tumor tissues and normal tissues. It is possible to distinguish invasive tumors from normal tissues by using Raman spectroscopy.

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Endoscopic Raman spectroscopy enables objective diagnosis of dysplasia in Barrett's esophagus

Cited by 107 publications

References 21 publications

Biomedical optical spectroscopy for the early diagnosis of gastrointestinal neoplasms

Biomedical optical spectroscopy for the early diagnosis of gastrointestinal neoplasms

In vivowhite light and contrast-enhanced vital-dye fluorescence imaging of Barrett’s-related neoplasia in a single-endoscopic insertion

Assessment of Tumor Cells in a Mouse Model of Diffuse Infiltrative Glioma by Raman Spectroscopy

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