Endoscopic topical application of Ankaferd Blood Stopper for neoplastic gastrointestinal bleeding: A retrospective analysis

Kurt, Mevlüt; Akdoğan, Meral; Önal, İbrahi̇m Koral; Kekilli, Murat; Arhan, Mehmet; Shorbagi, Ali; Aksu, Salih; Kurt, Ozlem Kar; Haznedaroğlu, İbrahim C.

doi:10.1016/j.dld.2009.05.006

Cited by 77 publications

(69 citation statements)

References 10 publications

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“…The hemostatic action of ABS is correlated with a reduction in tumor neo-angiogenesis. Topical ABS administration to gastrointestinal neoplastic tissue resulted in the control of bleeding and decreased tumor vascularization in rectal and gastric cancers [26]. Moreover, there is a close relationship between coagulation factor expression and solid tumor progression, via mechanisms other than angiogenesis.…”

Section: Yy1mentioning

confidence: 99%

“…A phase I double-blind, randomized crossover, placebo-controlled clinical study with a 5-d washout period between the crossover periods that included healthy volunteers reported that ABS was safe [20]. Physiological cell-based coagulation was clinically managed using topical ABS to prevent and treat bleeding associated with many clinicopathological states [10,18,[26][27][28]. Erythrocyte masses are critical in the ABS-induced hemostatic protein network (Figures 4 and 5).…”

Section: Nf-κbmentioning

confidence: 99%

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The effects of Ankaferd Blood Stopper on transcription factors in HUVEC and erythrocyte protein profile

Yılmaz¹,

Güleç²,

Torun³

et al. 2011

Turk J Hematol

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Objective: Ankaferd ® Blood Stopper (ABS) is an herbal extract that has historically been used as a hemostatic agent in traditional Turkish medicine. ABS is comprised of a standardized herbal mixture of T. vulgaris, G. glabra, V. vinifera, A. officinarum, and U. dioica. ABS's basic mechanism of action is the formation of an encapsulated protein web, which represents the focal point for vital erythrocyte masses. The hemostatic effects of ABS have been observed in vitro and in vivo. ABS was registered as a hemostatic agent for external hemorrhages and dental bleeding following phase I randomized, double-blind crossover placebo-controlled clinical research, and safety and efficacy reports. In terms of the potential use of ABS, transcription factors may be novel factors that play a role in the hemostatic and other pleiotropic effects of ABS. Materials and Methods: Hence, the present study aimed to investigate the effects of ABS on endothelium, and possible transcription factor changes in HUVEC (human umbilical vein endothelial cells) and the erythrocyte membrane profile. ABS (5 μL and 50 μL) was administered to HUVEC (in 75 cm 2 ; ~75% fullness) for 5 min and 15 min. Results: ABS caused significant increases in the level of activation of the following transcription factors; AP2, AR, CRE/ATF1, CREB, E2F1-5, E2F6, EGR, GATA, HNF-1, ISRE, Myc-Max, NF-1, NFkB, p53, PPAR, SMAD 2/3, SP1, TRE/AP1, and YY1. Following erythrocyte membrane isolation, protein complexes were undissolved, but denatured. The protein complex formed was resistant to heat and detergent. Trypsin and sonication were used in order to break this complex; the complex dissolved and erythrocyte membrane proteins were released in SDS-PAGE. Conclusion: ABS established a very fast and solid protein web, and increased the level of transcription factor activation. Therefore the cellular effects of ABS could be related to different intracellular biological pathways. (Turk J Hematol 2011; 28: 276-85)

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Section: Yy1mentioning

confidence: 99%

Section: Nf-κbmentioning

confidence: 99%

The effects of Ankaferd Blood Stopper on transcription factors in HUVEC and erythrocyte protein profile

Yılmaz¹,

Güleç²,

Torun³

et al. 2011

Turk J Hematol

Self Cite

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“…[6][7][8][9][10][11][12][13][14][15][16][17][18][19][20][21][22] It affects not only hemostasis but also wound healing with some antibacterial properties. [7][8][16][17][18][19][34][35][36][37][38][39] Clinical conditions of ABS use are increased in course of time as hemorrhagic problems persist despite available anti-hemorrhagic interventions.…”

Section: Discussionmentioning

confidence: 99%

“…5 Since ABS is a relatively novel hemostatic agent, the experience regarding its use in distinct hemorrhagic states is expanding. [6][7][8][9][10][11][12][13][14][15][16][17][18][19][20][21][22] ABS-induced formation of the protein network with vital erythroid aggregation covers the entire physiological hemostatic process. 6,[22][23][24] Mainly, there are distinct important components of the ABS-induced hemostatic network.…”

Section: K Ke Ey Y Wmentioning

confidence: 99%

Effects of Ankaferd Hemostat on Retinal Apoptosis in an Experimental Rabbit Retinal Model

Çıtırık¹,

Dilsiz²,

Haznedaroğlu³

et al. 2013

Turkiye Klinikleri J Med Sci

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836nkaferd is an herbal extract, which has been used historically as a hemostatic agent in traditional Turkish medicine for centuries. The medicine comprised the standardized mixture of herbs Thymus vul- Effects of Ankaferd Hemostat on Retinal Apoptosis in an ExperimentalRabbit Retinal Model A AB BS ST TR RA AC CT T O Ob bj je ec ct ti iv ve e: : To assess ultrastructural alterations and cellular apoptotic effects of Ankaferd Blood Stopper ® (ABS) in an experimental rabbit retinal model. Retinal changes following the intravitreal injection of ABS were investigated on ten retina tissues of five rabbits. M Ma at te er ri ia al l a an nd d M Me et th ho od ds s: : ABS was injected into the vitreous of right eye of five rabbits. The left eyes of rabbits received an intravitreal injection of balanced salt solution and were used as the control group. Oxidative damage, apoptosis, protein carbonylation and DNA fragmentation were studied. R Re es su ul lt ts s: : In the ABS injected eyes, the results of sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) pattern of a few protein bands were up-regulated whereas some other bands showed down-regulation. The apoptosis was delayed, indicated by the morphological analyses and caspase activity in the ABS injected eyes. Furthermore, ABS-induced protein carbonyle formation and DNA fragmentation were also demonstrated in the rabbit retina. C Co on nc cl lu us si io on n: : ABS has de novo effects on apoptosis. ABS-induced down-regulations in the expressions of apoptotic molecules in the retinal microenvironment indicated that ABS might act as a topical biological response modifier. Intravitreal injection of ABS also has cytotoxic effects. Since ABS is currently being developed in basic and clinical grounds, those novel observations will guide future studies focusing on the pleiotropic effects of this unique hemostatic agent. K Ke ey y W Wo or rd ds s: : Ankaferd blood stoper; apoptosis; DNA fragmentation; intravitreal injections; protein carbonylation; retina Ö ÖZ ZE ET T A Am ma aç ç: : Deneysel tavşan retina modelinde Ankaferd Kan Durdurucu ® (ABS)'nun hücresel apopitotik etkisini ve neden olduğu ince yapısal değişiklikleri değerlendirmek. ABS'nin intravitreal enjeksiyonundan sonra beş tavşandaki on retina dokusu üzerinde meydana gelen değişiklikler incelendi. G Ge er re eç ç v ve e Y Yö ön nt te em ml le er r: : Beş tavşanın sağ gözüne vitreus içine ABS enjekte edildi. Tavşanların sol gözleri kontrol grubu olarak kullanıldı ve vitreus içine dengeli tuz solüsyonu enjekte edildi. Oksidatif hasar, apopitoz, protein karbonilasyonu ve DNA fragmantasyonu incelendi. B Bu ul lg gu ul la ar r: : ABS enjekte edilen gözlerde, sodyum dodesil sülfat poliakrilamid jel elektroforezi (SDS-PAGE)'nde birkaç protein bandında artma ve bazı protein bantlarında azalma görüldü. ABS enjekte edilen gözlerde kaspaz aktivitesi ve morfolojik analizler ile tespit edilen apopitozda gecikme belirlendi. Ayrıca, tavşan retinalarında ABS'nin indüklediği protein karbonilasyonu oluşumu ve DNA fr...

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“…[13] ABS has no toxic reactions when applied to mucosal surfaces like the mouth and through the gastrointestinal tract. [13][14][15][16][17] However, the effects of longterm and deep tissue exposure to ABS remain unclear in these studies.…”

Section: Sonuçmentioning

confidence: 99%

The histopathological investigation of the effect on regional and systemic tissues of the application of Medicinal Plant Extract Ankaferd Blood Stopper in deep tissue injuries in Rats

Okumuş¹,

Yüksel²,

Özbağ³

et al. 2013

Ulus Travma Acil Cerrahi Derg

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Endoscopic topical application of Ankaferd Blood Stopper for neoplastic gastrointestinal bleeding: A retrospective analysis

Cited by 77 publications

References 10 publications

The effects of Ankaferd Blood Stopper on transcription factors in HUVEC and erythrocyte protein profile

The effects of Ankaferd Blood Stopper on transcription factors in HUVEC and erythrocyte protein profile

Effects of Ankaferd Hemostat on Retinal Apoptosis in an Experimental Rabbit Retinal Model

The histopathological investigation of the effect on regional and systemic tissues of the application of Medicinal Plant Extract Ankaferd Blood Stopper in deep tissue injuries in Rats

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