Endoscopic transcervical intrauterine artificial insemination in Labrador Retriever bitches

Macedo, Sabrina Pereira; Malm, Christina; Henry, M.; Telles, Luiz Flávio; Figueiredo, María Marta; Fukushima, Fabíola Bono; Machado‐Neves, Mariana; Cavalcanti, Guilherme Albuquerque de Oliveira; Chaves, Marcela Silva; Mascarenhas, Rebeca Marques; Lagares, Monique de Albuquerque; Gheller, Valentim Arabicano

doi:10.1016/j.rvsc.2011.04.015

Cited by 6 publications

(8 citation statements)

References 15 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In this study, slight pain was observed, but TCI could be easily performed without anesthesia in all bitches. In addition, procedural time was approximately 8 min, which was shorter than a previous report using a rigid endoscope [13]. This result was considered, because of a good view obtained by air insufflation and higher operability by using an instrument channel as well as increased mobility of the endoscope tip.…”

Section: Discussionmentioning

confidence: 80%

“…Among the many TCI reports, the method using a rigid endoscope is relatively easy and non-invasive [13]. However, flexible endoscopes are widely used in the field of gastroenterology, and are less invasive than a rigid cystoscope because they are soft [21,22,29].…”

Section: Discussionmentioning

confidence: 99%

“…The conception rate in a TCI study that used a rigid endoscope was 90% with 5 fetuses [13]. In addition, the conception rate and number of fetuses are about the same for intravaginal AI with fresh semen [6,15].…”

Section: Discussionmentioning

confidence: 99%

“…Surgical insemination is highly invasive and requires general anesthesia, whereas transcervical insemination (TCI) using metal catheterization is minimally invasive but requires a skilled technique [24]. In contrast, TCI using a rigid endoscope is easy and less invasive [13,30]. TCI with a flexible endoscope is also less invasive because the scope is soft, and it has an air supply and instrument channel, which endows it with good operability [21,29].…”

mentioning

confidence: 99%

See 3 more Smart Citations

Evaluation of Transcervical Insemination using Frozen Semen by Flexible Endoscope in Dogs

Hayashi

Morita

Aso

et al. 2013

The Journal of Veterinary Medical Science

View full text Add to dashboard Cite

ABSTRACT. We evaluated transcervical insemination (TCI) using frozen semen by flexible endoscopy in dogs. Eight female and eight male beagles were used in this study. A flexible endoscope and a washing tube were used for TCI. A tracheal tube was used as an alternative to the penis and was an auxiliary for inserting the flexible endoscope. The mean time required to insert the washing tube into the external os of the uterus after inserting the endoscope into the tracheal tube was 7.5 min. Slight or mild pain was observed in all bitches during TCI. However, TCI could be easily performed with retention in all bitches and without sedation anesthesia. The tracheal tube was useful to ensure the visual field using air sufflation. Clinical signs suspicious of infection were not observed in any bitches from the TCI to the pregnancy diagnosis. The conception rate was 87.5%, and the mean number of fetuses was 6.3. TCI using a flexible endoscope in bitches was performed quickly with minimal invasiveness. We present a new method of TCI in dogs. This method should be studied in small and large breeds to obtain more detailed results. Artificial insemination (AI) using frozen semen in dogs has been increasing in recent years. However, AI using cryopreserved semen generally yields lower pregnancy rates with vaginal deposition than with uterine deposition because of the short life span of frozen-thawed spermatozoa [5,19]. Therefore, AI methods usually use uterine deposition with frozen semen. Surgical insemination is highly invasive and requires general anesthesia, whereas transcervical insemination (TCI) using metal catheterization is minimally invasive but requires a skilled technique [24]. In contrast, TCI using a rigid endoscope is easy and less invasive [13,30]. TCI with a flexible endoscope is also less invasive because the scope is soft, and it has an air supply and instrument channel, which endows it with good operability [21,29]. Therefore, a flexible endoscope might be useful as a new tool for TCI.The purpose of this study was to evaluate TCI using frozen semen by flexible endoscope in dogs. MATERIALS AND METHODSEight female beagles aged 1-8 years (mean ± standard deviation: SD, 4.1 ± 2.5 years) and weighing 10.7-15.1 kg (mean ± SD, 12.8 ± 1.3 kg) were used in this study; 4 were parous, and the others were nulliparous. Eight male beagles aged 1-7 years (mean ± SD, 3.6 ± 1.8 years) and weighing 11.4-15.8 kg (mean ± SD, 13.1 ± 1.4 kg) were used as sperm donors. Unrelated dogs were used to prevent inbreeding. This study was performed in accordance with the Guide for the Experimentation of Animals of the College of Bioresource Sciences, Nihon University (AP11B018, 2010).The sperm-rich fraction of a single ejaculate was collected from each dog by digital manipulation [11]. The collected semen was centrifuged at 400 × g for 5 min. The supernatant was discarded, and the pellets were gently suspended in 1 ml with egg-yolk Tris-fructose citrate extender supplemented with 1% Orvus ES paste (OEP) [26][27][28]. Sperm concentration w...

show abstract

Section: Discussionmentioning

confidence: 80%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

See 2 more Smart Citations

Evaluation of Transcervical Insemination using Frozen Semen by Flexible Endoscope in Dogs

Hayashi

Morita

Aso

et al. 2013

The Journal of Veterinary Medical Science

View full text Add to dashboard Cite

show abstract

“…Normally up to 80% of bitches can be inseminated without any difficulty in catheterization, while in the remaining 20% of cases varying degrees of cervical tubercle manipulation with the endoscope and the catheter may be necessary (Macedo et al. ). The mean time necessary to catheterize the cervix can be as short as 7.5 min (Hayashi et al.…”

Section: Transcervical Catheterizationmentioning

confidence: 99%

Transcervical Artificial Insemination in Dogs and Cats: Review of the Technique and Practical Aspects

Romagnoli

Lopate

2014

Reprod Domestic Animals

View full text Add to dashboard Cite

Contents The technique of transcervical catheterization for artificial insemination has gained practical importance over the last 3 and 1 decades in bitches and queens, respectively. The vagina of both species has a thickening of the dorsal aspect called dorsal medial fold, which restricts the lumen of the paracervix, making catheterization of the cervix difficult both with manual and endoscopic techniques. Manual catheters have been used initially in the bitch and are now gradually being replaced by rigid endoscopy through the adaptation of human cystoscopes and ureteroscopes. Cystoscopes provide excellent imaging of the vagina, but cervical catheterization is more difficult due to the oblique 30° viewing angle of its telescope and may not be long enough to catheterize large size bitches. Ureteroscopes allow an acceptable view of the vaginal mucosa and offer advantages such as the possibility to catheterize the cervix of bitches of all body sizes, manipulate the cervical tubercle when the external cervical os is not visible, offer a better visualization of the cervix using the shunt (a large Foley catheter which allows insufflation and distention of the vagina). Feline cervical catheterization has been achieved with three different types of catheters; the last one, developed following careful anatomical studies of how the feline vaginal lumen changes during estrus, allows cervical catheterization thanks to digital manipulation of the cervix through the rectum.

show abstract

Vaginal Endoscopy in the Bitch

Maenhoudt

Santos

2021

Veterinary Endoscopy for the Small Animal Practitioner

View full text Add to dashboard Cite

Endoscopic transcervical intrauterine artificial insemination in Labrador Retriever bitches

Cited by 6 publications

References 15 publications

Evaluation of Transcervical Insemination using Frozen Semen by Flexible Endoscope in Dogs

Evaluation of Transcervical Insemination using Frozen Semen by Flexible Endoscope in Dogs

Transcervical Artificial Insemination in Dogs and Cats: Review of the Technique and Practical Aspects

Vaginal Endoscopy in the Bitch

Contact Info

Product

Resources

About