Endosomal escape efficiency of fusogenic B18 and B55 peptides fused with anti-EGFR single chain Fv as estimated by nuclear translocation

Niikura, Keisuke; Horisawa, Kenichi; Doi, Nobuhide

doi:10.1093/jb/mvv083

Cited by 10 publications

(9 citation statements)

References 37 publications

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“…We observed a focal pattern of EGFP and Cas9 fluorescence in ovaries following injection with P2C-EGFP-Cas9 (Fig. 2 ) and the body of literature surrounding drug delivery efforts supports the confinement of cargo to endosomes following RME 13 , 20 , 24 – 26 , 34 . We therefore sought to explore whether EERs characterized in these drug studies would promote editing of the germline DNA by mediating the release of the P2C-EGFP-Cas9 from endosomes.…”

Section: Resultssupporting

confidence: 68%

Targeted delivery of CRISPR-Cas9 ribonucleoprotein into arthropod ovaries for heritable germline gene editing

et al. 2018

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Cas9-mediated gene editing is a powerful tool for addressing research questions in arthropods. Current approaches rely upon delivering Cas9 ribonucleoprotein (RNP) complex by embryonic microinjection, which is challenging, is limited to a small number of species, and is inefficient even in optimized taxa. Here we develop a technology termed Receptor-Mediated Ovary Transduction of Cargo (ReMOT Control) to deliver Cas9 RNP to the arthropod germline by injection into adult female mosquitoes. We identify a peptide (P2C) that mediates transduction of Cas9 RNP from the female hemolymph to the developing mosquito oocytes, resulting in heritable gene editing of the offspring with efficiency as high as 0.3 mutants per injected mosquito. We demonstrate that P2C functions in six mosquito species. Identification of taxa-specific ovary-specific ligand–receptor pairs may further extend the use of ReMOT Control for gene editing in novel species.

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Section: Resultssupporting

confidence: 68%

Targeted delivery of CRISPR-Cas9 ribonucleoprotein into arthropod ovaries for heritable germline gene editing

et al. 2018

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“…B18 represents the “minimal membrane‐binding and fusogenic motif.” 120 Both peptides, B18 and B55, can self‐assemble in lipid bilayers and transport biomacromolecules. Thus, an enhanced green fluorescent protein (EGFP)‐fused B55 peptide was developed to promote the intracellular uptake of coadministered biomacromolecules such as dextrans, monoclonal single‐chain antibodies, and even pDNA into human cell lines 120,122 . Moreover, both peptides can perform not only endosomal escape but also nuclear translocation 120 …”

Section: Cell‐penetrating Proteins With Specific Functional Propertiesmentioning

confidence: 99%

New generation of cell‐penetrating peptides: Functionality and potential clinical application

Reißmann

Filatova

2021

Journal of Peptide Science

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Cell‐penetrating peptides (CPPs) can transport various cargoes through membranes of live cells. Since the first generations of CPPs suffered from insufficient cell and tissue selectivity, stability against proteases, and escape from endosomes, a new generation of peptides, with optimized properties, was developed. These are either derived from natural sources or created through the combination of multivalent structures. The second method allows achieving high internalization efficiency, high cell and tissue selectivity, and release from endosomes via hybrid structures, combining sequences for endosomal release, homing sequences, and sequences for activation at the target tissue and for local delivery of cargoes. CPPs with innate tumor selectivity include azurin, crotamine, maurocalcine, lycosin‐I, buffalo cathelicidin, and peptide CB5005. Some of them can penetrate the membranes of live cells and influence intracellular signaling pathways, thereby exerting cytotoxic effects against tumor cells. To obtain multilayer penetration and stabilization against proteolytic degradation, as well as for better handling, CPPs are often conjugated to nanoparticles. A special problem for tumor treatment is the efficiency of drug transport through three‐dimensional cell cultures. Therefore, the capability of CPPs to deliver the drug even to the innermost tissues is of crucial importance. Notably, the ability of certain CPPs to penetrate barriers such as skin, the blood‐brain barrier (BBB), and cornea or conjunctiva of eyes enabled the replacement of dangerous and painful injections with soothing sprays, creams, and drops. However, it is difficult to rank the efficacy of CPPs because transport efficiency and tissue selectivity depend not only on the CPP itself but also on the target tissue or organ, as well as on the cargo and method of CPP‐cargo coupling. Therefore, the present review describes some examples of new‐generation CPPs and aims to provide advice on how to find or create the right CPP for a given task.

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“…The firstly described cell-penetrating peptides were a substance P analog [6], HIV-TAT (47)(48)(49)(50)(51)(52)(53)(54)(55)(56)(57) derived from TAT-protein of virus HIV-1 [7] and penetratin, a peptide derived from Drosophila antennapedia gene homeobox [8].…”

Section: First and Next Generations First Generationmentioning

confidence: 99%

“…From TAT-protein of virus HIV-1 [7] various sequences were derived and tested for their uptake efficiency. Commonly used is the sequence HIV-TAT (47)(48)(49)(50)(51)(52)(53)(54)(55)(56)(57).…”

Section: Cationic Cpps Hiv-tatmentioning

confidence: 99%

“…One way was developing peptides with alternating chirality of amino acid residues. Thus such cationic peptides like HIV-TAT (47)(48)(49)(50)(51)(52)(53)(54)(55)(56)(57) and octa-arginine with alternating chirality of arginine residues were released from endosomes into cytosol [39]. This effect is due to changed conformation and changed conformational flexibility.…”

Section: Endosomal Leakage Structuresmentioning

confidence: 99%

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State of Art: Cell Penetration and Cell-Penetrating Peptides and Proteins

2021

Health Educ Public Health

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Cell-penetrating peptides and proteins (CPPs) are compounds which can penetrate live cells and can transport cargoes and labels into the cell or even into subcellular organelles. Some cells are easy to transduce or to transfect, other cells only difficult or not to transfect. Uptake efficiency can strongly depend on CPP, on the cargo, cell type and used conditions. CPPs are very effective in transporting peptides, proteins, hydrophilic bioactive synthetic or natural compounds. Furthermore, they are tools for transporting drugs, including herbal products from classical chine´s medicine. CPPs are also able to transport ribonucleic acids and derivatives of them like peptide nucleic acids or morpholino oligomers. CPPs are also able to penetrate barriers like blood-brain barrier, conjunctiva of eye, skin,

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Endosomal escape efficiency of fusogenic B18 and B55 peptides fused with anti-EGFR single chain Fv as estimated by nuclear translocation

Cited by 10 publications

References 37 publications

Targeted delivery of CRISPR-Cas9 ribonucleoprotein into arthropod ovaries for heritable germline gene editing

Targeted delivery of CRISPR-Cas9 ribonucleoprotein into arthropod ovaries for heritable germline gene editing

New generation of cell‐penetrating peptides: Functionality and potential clinical application

State of Art: Cell Penetration and Cell-Penetrating Peptides and Proteins

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