Endosperm culture: a facile and efficient biotechnological tool to generate passion fruit (Passiflora cincinnata Mast.) triploid plants

Silva, Nayara Tayane da; Silva, Lázara Aline Simões; Reis, Aryane Campos; Machado, Mariana; Matos, Elyabe Monteiro de; Viccini, Lyderson Facio; Otoni, Wagner Campos; Carvalho, Ilio Fealho de; Rocha, Diego Ismael; Silva, Maurecilne Lemes da

doi:10.1007/s11240-020-01887-2

Cited by 16 publications

(11 citation statements)

References 62 publications

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“…FCM has been used to con rm the endosperm origin of regenerated plants, for example, of A. chinensis var. deliciosa (Góralski et al 2005), Gomortega keule (Muñoz-Concha 2016), A. kolomikta (Asakura and Hoshino 2017), Melia azedazach (Thang et al 2018), and Passi ora cincinnata (Silva et al 2020). The FCM analysis done in this study on A. arguta regenerants from endosperm-derived calli, obtained from both sources of explants, con rmed the hexaploidy (6Cx = 3C DNA content) of the regenerants through a comparison with the tetraploid seedlings of a commercially available tetraploid cultivar.…”

Section: Discussionmentioning

confidence: 99%

“…Furthermore, the plant growth regulators (PGRs) added to the culture medium, the sources of the explant material, and the cultivar of the donor plants all play an important role in establishing a successful regeneration protocol from endosperm explants (Wang et al 2016). Despite being challenging work, the recently published protocols for endosperm-derived plant regeneration in Gomortega keule (Muñoz-Concha 2016), Melia azedazach (Thang et al 2018), Passi ora edulis (Antoniazzi et al 2018), and Passi ora cincinnata (Silva et al 2020) indicate that this technique is still attracting interest and is worth applying to economically important plant species.…”

Section: Introductionmentioning

confidence: 99%

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Kiwiberry (Actinidia Arguta) Hexaploid Plant Regeneration Through Culture of Endosperm Isolated From Fresh and Year-old Dry Seeds

Abdullah¹,

Śliwińska²,

Góralski³

et al. 2021

Preprint

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Endosperm, an ephemeral and storage tissue, serves as a source of nutrition and protection during embryo development and germination. It can be used for the cultivation of polyploid plants in vitro. Here, a protocol of plant regeneration and acclimatization from the endosperm-derived calli of Actinidia arguta has been developed. Seeds excised from fresh fruit and dry seeds stored for one year served as the sources of endosperm explants of selected tetraploid cultivars of A. arguta. Callus Induction Medium (CIM; containing 0.25, 0.5, or 1 mg/l of TDZ) and Actinidia Endosperm Medium (AEM; containing 2 mg/l of 2,4-D and 5 mg/l of kinetin) were used to study the organogenic responses of the calli. On AEM, the source of explant did not significantly affect the rate of callus induction for any of the tested cultivars. Similarly, no organogenic events were observed. In contrast, on CIM both the source of explants and the cultivar origin caused significant differences in callus formation and subsequent organogenic events. Histological and ultrastructural analyses revealed the adventitious nature of shoot bud formation on these media. The most efficient elongation of shoot buds was achieved after transferring organogenic calli with adventitious shoot buds to a medium supplemented with zeatin or meta-topolin. Robust root induction with minimal basal callus formation occurred on the medium with indole-3-acetic acid. Flow cytometric analysis revealed that the nuclear DNA content in the leaves of some regenerants (4.5 pg/2C) was approximately 50% higher than that in the tetraploid seedlings (3.1 pg). This finding confirmed that those regenerants originated from the endosperm. The regeneration of hexaploid plants was more efficient when endosperm from fresh seeds served as an explant; therefore, fresh rather than dry seeds are recommended for endosperm-derived plant production. The hexaploid plants of A. arguta can serve as an important source of breeding material.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Kiwiberry (Actinidia Arguta) Hexaploid Plant Regeneration Through Culture of Endosperm Isolated From Fresh and Year-old Dry Seeds

Abdullah¹,

Śliwińska²,

Góralski³

et al. 2021

Preprint

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“…The method has been used to confirm the endosperm origin of regenerated plants, for example, of A. chinensis var. deliciosa (Góralski et al 2005), Gomortega keule (Muñoz-Concha 2016), A. kolomikta (Asakura and Hoshino 2017), Melia azedazach (Thang et al 2018), and Passiflora cincinnata (Silva et al 2020). For Actinidia, the range of DNA content of 2C nucleus for tetraploids was reported as 2.83-3.15 pg (an average 2.96 pg) (Start et al 2007).…”

Section: Nuclear Dna Content and Dna-ploidymentioning

confidence: 99%

“…Furthermore, the plant growth regulators (PGRs) added to the culture medium, the sources of the explant material, and the cultivar of the donor plants all play an important role in establishing a successful regeneration protocol from endosperm explants (Wang et al 2016). Despite being challenging task, the recently published protocols for endosperm-derived plant regeneration in Gomortega keule (Muñoz-Concha 2016), Melia azedazach (Thang et al 2018), Passiflora edulis (Antoniazzi et al 2018), and Passiflora cincinnata (Silva et al 2020) indicate that this technique is still attracting interest and is worth applying to economically important plant species.…”

Section: Introductionmentioning

confidence: 99%

Effect of medium composition, genotype and age of explant on the regeneration of hexaploid plants from endosperm culture of tetraploid kiwiberry (Actinidia arguta)

Abdullah

Śliwińska

Góralski

et al. 2021

Plant Cell Tiss Organ Cult

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Endosperm, an ephemeral and storage tissue, serves as a source of nutrition and protection during embryo development and germination. It can be used for the cultivation of polyploid plants in vitro. Here, results of plant regeneration and acclimatization from the endosperm-derived calli of four cultivars of Actinidia arguta has been presented. Seeds excised from fresh fruit and dry seeds stored for one year served as the sources of endosperm explants of selected tetraploid cultivars of A. arguta. Callus Induction Medium (CIM; containing 0.25, 0.5, or 1 mg/l of TDZ) and Actinidia Endosperm Medium (AEM; containing 2 mg/l of 2,4-D and 5 mg/l of kinetin) were used to study the organogenic responses of the calli. On AEM, the source of explant did not significantly affect the rate of callus induction for any of the tested cultivars; no organogenic events were observed. In contrast, on CIM both the source of explants and the cultivar origin caused significant differences in callus formation and subsequent organogenic events. Histological and ultrastructural analyses revealed the adventitious nature of shoot bud formation on these media. The most efficient elongation of shoot buds was achieved after transferring organogenic calli with adventitious shoot buds to a medium supplemented with zeatin or meta-topolin. Robust root induction with minimal basal callus formation occurred on the medium with indole-3-acetic acid. Flow cytometric analysis revealed that the nuclear DNA content in the leaves of some regenerants was approximately 50 % higher (4.5 pg/2C) than that in leaves from the tetraploid seedlings (3.1 pg/2C),which confirmed that those regenerants originated from the endosperm. The regeneration of such hexaploid plants was more efficient when endosperm from fresh seeds served as an explant; therefore, fresh rather than dry seeds are recommended for endosperm-derived plant production. The hexaploid plants of A. arguta can serve as an important source of breeding material.

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“…Thus, in vitro cultivation techniques support propagation with e cient multiplication protocols in cases of low germination. For the Passi ora genus, these protocols are developed by combining explant types (Antoniazzi et al, 2018;Silva et al, 2020), the growth regulators (Faria et al, 2018), and the culture environment (Vieira et al, 2014).…”

Section: Introductionmentioning

confidence: 99%

BA and TDZ in the Morphogenesis in Vitro of Passiflora Setacea

Araújo

Carvalho

Generoso

et al. 2022

Preprint

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Passiflora setacea DC (Passifloraceae) is considered an important species in the genetic breeding of passion fruit. However, its use is limited due to low seed germination. This paper aimed to study the effect of cytokinins 6-benzyladenine (BA) and thidiazuron (TDZ) on the in vitro morphogenesis of P. setacea using three explants: hypocotyl, nodal segment, and root segment. The explants were induced to morphogenesis in MS medium modified and with different concentrations of BA and TDZ. After 55 days, the percentage of calluses and shoots were evaluated, and anatomical characterization was performed. The three explants used in the in vitro morphogenesis of P. setacea showed callus and shoots formation, but in greater numbers in the nodal segments treated with BA. TDZ isolated affected equal to or less than BA on callus and shoots formation for the three explants. Direct and indirect organogenesis was observed in the three types of explants. From the results obtained for plant regeneration via in vitro morphogenesis of P. setacea, it is recommended to use a nodal segment in MSM medium and supplemented with 2.22 μmol L-1 of BA.

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Endosperm culture: a facile and efficient biotechnological tool to generate passion fruit (Passiflora cincinnata Mast.) triploid plants

Cited by 16 publications

References 62 publications

Kiwiberry (Actinidia Arguta) Hexaploid Plant Regeneration Through Culture of Endosperm Isolated From Fresh and Year-old Dry Seeds

Kiwiberry (Actinidia Arguta) Hexaploid Plant Regeneration Through Culture of Endosperm Isolated From Fresh and Year-old Dry Seeds

Effect of medium composition, genotype and age of explant on the regeneration of hexaploid plants from endosperm culture of tetraploid kiwiberry (Actinidia arguta)

BA and TDZ in the Morphogenesis in Vitro of Passiflora Setacea

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