Endospore production allows using spray-drying as a possible formulation system of the biocontrol agent Bacillus subtilis CPA-8

Abstract

The role of endospore production by Bacillus subtilis CPA-8 on survival during spray-drying was investigated by comparison with a non-spore-forming biocontrol agent Pantoea agglomerans CPA-2. Endospore formation promoted heat resistance in CPA-8 depending on growth time (72 h cultures were more resistant than 24 h ones). The survival of CPA-8 and CPA-2 after spray-drying was determined after being grown in optimised media for 24 and 72 h. Spray-dried 72 h CPA-8 had the best survival (32%), while CPA-2 viability was less than 2%. CPA-8 survival directly related with its ability to produce endospores. Spray-dried CPA-8 reduced Monilinia fructicola conidia germination similarly to fresh cells, demonstrating that spray-drying did not adversely affect biocontrol efficacy. Endospore production thus improves CPA-8 resistance to spray-drying. These results can provide a reliable basis for optimising of the spray-drying formulation process for CPA-8 and other microorganisms.

Co-inoculation of Liquid Microbial Cultures and Compatibility with Chemicals for Improvement of Seed Germination and Vigour in Paddy

“…have proven to be ideal candidates for development as stable and efficient biological products because their ability to produce heat-resistant endospores (Yanez-Mendizabal et al, 2012) which survive the stresses of commercial seed treatment better than nonspore-forming species such as Pseudomonas spp. Mehta et al, (2011) opined that the combined application of Zn, biofertilizers and pesticides is possible to increase yield due to early nourishment through Zn and biofertilizers as well as insecticides.…”

Biological control of brown rot in stone fruit using Bacillus amyloliquefaciens CPA-8 under field conditions

“…and Botrytis cinerea (Gotor-Vila et al, 2017a). As part of the genus Bacillus, its spore forming ability also provides high resistance to extreme environmental conditions, making CPA-8 a good candidate for developing stable and efficient BCA products (Gotor-Vila et al, 2017c ;Yánez-Mendizábal et al, 2012a).…”

Formulation of the biocontrol agent Bacillus amyloliquefaciens CPA-8 using different approaches: liquid, freeze-drying and fluid-bed spray-drying

“…The efficacy of the BCA Bacillus amyloliquefaciens CPA-8, formerly known as B. subtilis (Gotor-Vila et al, 2016), has been previously described against postharvest diseases caused by Monilinia spp. based on its ability to produce fengycin-like lipopeptides (Yánez-Mendizábal et al, 2012a;Yánez-Mendizábal et al, 2012d) One of the major bottle necks in the commercialisation of biocontrol products is the development of shelf-stable formulations (Droby et al, 2009;Navarta et al, 2011). A useful microbial formulation should be economical to produce, easy to distribute to the intended environment, contain enough colony forming units (CFU), and provide a long shelf-life (preferentially stored at room temperatures and maintained for at least six months) (Melin et al, 2007;Teixidó et al, 2011).…”