Endosymbiotic and Host Proteases in the Digestive Tract of the Invasive Snail Pomacea canaliculata: Diversity, Origin and Characterization

Godoy, Martín S.; Castro-Vasquez, Alfredo; Vega, Israel A.

doi:10.1371/journal.pone.0066689

Cited by 22 publications

(37 citation statements)

References 45 publications

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“…The temperature optimum of the stenothermal Antarctic shrimp Chorismus antarcticus is 40 °С (Dittrich, 1990). The temperature optimum of cathepsin D also varies considerably: the minimum values (35 ºС) were detected in the Pacific flying squid Todarodes pacificus (Sakai et al, 1981), higher values (50 ºС) -in larvae of the buzzer midge Chironomus plumosus (Kuz'mina, 1999). In all likelihood, the relatively low level of the peptidase maximum activity (40 ºС) in the chironomid larvae studied by us is due to the considerable activity of cathepsins.…”

Section: Discussionmentioning

confidence: 99%

Temperature characteristics of peptidase in chironomid larvae, potential fish prey, at various pH values

Кузьмина

Chornaya²,

Skvortsova

et al. 2018

Biosys. divers.

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The temperature dependence of casein- and hemoglobinlytic peptidases functioning in the whole organism of chironomid larvae Chironomus plumosus, food objects of adult benthophages and juvenile fish of various ecological groups, was studied within the temperature range of 0–70 ºС at different рН values (3.0, 5.0 and 7.4). The method of mixed samples was used to determine the activity and characteristics of enzymes. Homogenates of previously crushed and carefully mixed dozens of larvae were used as enzymatically active preparations. Activity of peptidases was assayed by the increase in tyrosine concentration using the Folin-Ciocalteu reagent. It is shown that the activity of peptidases that function in the tissues of chironomid larvae depends to a considerable extent on temperature and рН, but the pH has a smaller effect on the activity and the temperature dependence of casein- and hemoglobin-lytic peptidases than temperature. The temperature optimum of the studied peptidases of chironomid larvae corresponds to 40 ºС. The Q10 values in the zone of vital temperatures are slightly changed. They are, as a rule, increased in the zone of 30–40 ºС, and are sharply decreased in the zone of high temperatures. The values of activation energy of the process of hydrolysis of casein and hemoglobin in the zone of low and high temperatures are different. The Еact values of the process of hydrolysis of casein and hemoglobin at a temperature not exceeding 20 ºС are usually below those in the zone of higher temperatures (except for hemoglobin-lytic peptidases at pH 5.0). The data obtained indicate a significant effect of pH not only on the activity, but also on the temperature characteristics of peptidases that function in the body of chironomid larvae. Differences in the characteristics of casein- and hemoglobin-lytic peptidases in chironomid larvae at different temperatures and pH can influence the digestion in benthophages and fry of all fish species.

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Section: Discussionmentioning

confidence: 99%

Temperature characteristics of peptidase in chironomid larvae, potential fish prey, at various pH values

Кузьмина

Chornaya²,

Skvortsova

et al. 2018

Biosys. divers.

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“…Several proteases have been reported in P. canaliculata, including one produced by the salivary glands and two in the intestine; one associated with C and K morphotypes of an endosymbiont released from midgut gland cells, is ubiquitous in the digestive tract (Godoy et al, 2013) (see Symbiotic Associations).…”

Section: Digestionmentioning

confidence: 99%

Insights from an Integrated View of the Biology of Apple Snails (Caenogastropoda: Ampullariidae)

et al. 2015

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“…Х 2 values are shown when Kruskal-Wallis tests were performed for the effect of LPA only. a site of absorption but also of digestion, since proteases are freed to its lumen (Godoy et al, 2013), and this function may be related to an extended retention time relative to other parts of the digestive tract.…”

Section: Discussionmentioning

confidence: 99%

“…On the other hand, apple snails (Ampullariidae) are large snails mostly with macrophytophagous habits (Cazzaniga & Estebenet, 1984;Cowie, 2002;Marchese et al, 2014;Hayes et al, 2015) and they possess very powerful jaws and radula with which they shred living macrophyte tissues (Andrews, 1965;Moretto & Nahabedian, 1989;Martín & Negrete, 2007). They also possess a strongly muscular and cuticularized gizzard in the stomach (Andrews, 1965) and high levels of enzymatic activity in different parts of their digestive tract (Godoy et al, 2013;Luo et al, 2015). Even though these mechanical and chemical tools seem to be sufficient enough to process the relatively soft tissues of the aquatic plants usually consumed, Andrews (1965) described the presence of "small stones or grit" in the gizzard of Pomacea canaliculata (Lamarck, 1822), and suggested that they play a role in the disintegration of vegetal material.…”

Section: Introductionmentioning

confidence: 99%

Effects of Ingestion of Lithic Particles on Growth of the Apple SnailPomacea canaliculata(Caenogastropoda, Ampullariidae)

Manara¹,

Saveanu²,

Martín³

2016

Malacologia

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Lithic particles are a common feature in the digestive tract of freshwater snails. Their role in the digestive processes has been demonstrated in some microphytophagous and detritivorous species, as they enhance growth, assimilation and reproduction. It has been suggested that they could have the same function in Pomacea canaliculata, a macrophytophagous apple snail with powerful jaws and radula, a strongly muscular and cuticularized gizzard and high levels of enzymatic activity. Our aims were to investigate the occurrence of lithic elements in the digestive tract of P. canaliculata snails from natural populations through the analyses of digestive contents, as well as the effect of size and availability of lithic particles on growth and growth efficiency through laboratory experiments. Lithic particles are very common in the digestive tract of P. canaliculata from natural populations and from laboratory aquaria if they are available in the immediate environment. Such particles are not retained or concentrated differentially in the stomach and they are apparently totally lost in less than four weeks if the supply is interrupted. The frequency of plant material and lithic particles increases from mouth to anus indicating that the retention time increases in the same way. Sand and plant material frequently co-occur in the intestine and in the stomach indicating that both are ingested together. Ground marble had negative effects on the growth of P. canaliculata probably due to the sharp edges and pointed ends of these particles. The availability of natural lithic particles (sand) had a positive effect on growth and also a synergic interaction with the availability of food. The growth efficiency was 25.2% higher when sand was available than when it was absent. These effects were more marked in juvenile females than in juvenile males. Our results indicate that growth rates may be underestimated under laboratory conditions if lithic particles are not supplied regularly and that their presence should be standardized to allow reliable comparisons between studies. Our results also indicate that the effects of food availability and plant palatability on the growth of P. canaliculata may be modulated by the presence of lithic particles and this may in turn affect the outcome of interactions between apple snails, other snails and macrophytes.

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Endosymbiotic and Host Proteases in the Digestive Tract of the Invasive Snail Pomacea canaliculata: Diversity, Origin and Characterization

Cited by 22 publications

References 45 publications

Temperature characteristics of peptidase in chironomid larvae, potential fish prey, at various pH values

Temperature characteristics of peptidase in chironomid larvae, potential fish prey, at various pH values

Insights from an Integrated View of the Biology of Apple Snails (Caenogastropoda: Ampullariidae)

Effects of Ingestion of Lithic Particles on Growth of the Apple SnailPomacea canaliculata(Caenogastropoda, Ampullariidae)

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