Endothelial cell cytoskeletal alignment independent of fluid shear stress on micropatterned surfaces

Vartanian, Keri B.; Kirkpatrick, Sean J.; Hanson, Stephen R.; Hinds, Monica T.

doi:10.1016/j.bbrc.2008.04.167

Cited by 79 publications

(84 citation statements)

References 20 publications

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“…The micropattern surfaces of 25-m wide collagen lanes with 100-m wide spacing were created as previously described (29). Briefly, silicon templates were fabricated (Stanford Microfluidics Foundary) from photomylar masks (CAD/Art Services) at 20,000 dpi using photolithography.…”

Section: Methodsmentioning

confidence: 99%

“…Shape index and cytoskeletal structure. The shape indices and cytoskeletal components (actin and microtubules) were characterized as previously described (29). Briefly, with the use of Image J (NIH) on microscopy images of ECs, the surface areas and perimeters of ECs were measured for each condition.…”

Section: Methodsmentioning

confidence: 99%

“…The cytoskeletal components actin and microtubules were visualized using immunofluorescent staining with 1) mouse-anti-human ␤-tubulin (Sigma) primary antibody and Alexa Fluor 488 secondary antibody and 2) Alexa Fluor 568-conjugated Phalloidin (Invitrogen). The stained ECs were imaged at ϫ40 and a custom Matlab image analysis program was used to quantify the alignment of the stained cytoskeletal components as previously described (29). Briefly, the image was processed in two major steps: 1) Laplacian edge detection to highlight the cytoskeletal fibers and 2) radon transform to determine the distribution of fiber orientation angles.…”

Section: Methodsmentioning

confidence: 99%

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Cytoskeletal structure regulates endothelial cell immunogenicity independent of fluid shear stress

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Berny

McCarty

et al. 2010

American Journal of Physiology-Cell Physiology

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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Cytoskeletal structure regulates endothelial cell immunogenicity independent of fluid shear stress

Vartanian

Berny

McCarty

et al. 2010

American Journal of Physiology-Cell Physiology

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“…Also, our short term endothelial cell proliferation test results point in the direction of cytoskeleton organization e.g., actin aligning and it plays a very important role in the cellular function like cell proliferation [37,38]. The majority of the endothelial cells are aligned at an angle of 40-50° along the axis of the micropatterns.…”

Section: Morphology and Coverage Percentage Of Endothelial Cellsmentioning

confidence: 74%

Use of Micropatterned Thin Film Nitinol in Carotid Stents to Augment Embolic Protection

Shayan¹,

Jankowitz²,

Shridhar³

et al. 2016

Preprint

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Abstract:Stenting is an alternative to endarterectomy for the treatment of carotid artery stenosis. However, stenting is associated with a higher risk of procedural stroke secondary to distal thromboembolism. Hybrid stents with a micromesh layer have been proposed to address this complication. We developed a micropatterned thin film nitinol (M-TFN) covered stent designed to prevent thromboembolism during carotid intervention. This innovation may obviate the need or work synergistically with embolic protection devices. The proposed double layered stent is low-profile, thromboresistant, and covered with a M-TFN that can be fabricated with fenestrations of varying geometries and sizes. The M-TFN was created in multiple geometries, dimensions, and porosities by sputter deposition. The efficiency of various M-TFN to capture embolic particles was evaluated in different atherosclerotic carotid stenotic conditions through in vitro tests. The covered stent prevented emboli dislodgement in the range of 70-96% during 30min duration tests. In vitro vascular cell growth study results showed that endothelial cell elongation, alignment and growth behaviour silhouettes significantly enhance specifically on the diamond-shape M-TFN with the dimensions of 145µm×20µm and a porosity of 32%. Future studies will require in-vivo testing. Our results demonstrate that M-TFN has a promising potential for carotid artery stenting.

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“…(B) The development of improved micropatterning approaches allows the precise assessment of how surface topography influences the behavior of intracellular organelles or the cytoskeleton (Théry et al, 2006;Versaevel et al, 2012). The fast-evolving field of microfluidics has recently been used to assess how fluid forces (Perrault et al, 2015;Vartanian et al, 2008), mechanical confinement or topological constrictions Thiam et al, 2016) influence the behavior of cells. Measuring the migration time through confined 3D geometry provides information regarding cellular migration over topological constrictions .…”

Section: Optical Tweezers In Vivomentioning

confidence: 99%

Seeing is believing: multi-scale spatio-temporal imaging towards in vivo cell biology

Follain

Mercier

Osmani

et al. 2016

Journal of Cell Science

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Life is driven by a set of biological events that are naturally dynamic and tightly orchestrated from the single molecule to entire organisms. Although biochemistry and molecular biology have been essential in deciphering signaling at a cellular and organismal level, biological imaging has been instrumental for unraveling life processes across multiple scales. Imaging methods have considerably improved over the past decades and now allow to grasp the inner workings of proteins, organelles, cells, organs and whole organisms. Not only do they allow us to visualize these events in their most-relevant context but also to accurately quantify underlying biomechanical features and, so, provide essential information for their understanding. In this Commentary, we review a palette of imaging (and biophysical) methods that are available to the scientific community for elucidating a wide array of biological events. We cover the most-recent developments in intravital imaging, light-sheet microscopy, superresolution imaging, and correlative light and electron microscopy. In addition, we illustrate how these technologies have led to important insights in cell biology, from the molecular to the whole-organism resolution. Altogether, this review offers a snapshot of the current and state-of-the-art imaging methods that will contribute to the understanding of life and disease.

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Endothelial cell cytoskeletal alignment independent of fluid shear stress on micropatterned surfaces

Cited by 79 publications

References 20 publications

Cytoskeletal structure regulates endothelial cell immunogenicity independent of fluid shear stress

Cytoskeletal structure regulates endothelial cell immunogenicity independent of fluid shear stress

Use of Micropatterned Thin Film Nitinol in Carotid Stents to Augment Embolic Protection

Seeing is believing: multi-scale spatio-temporal imaging towards in vivo cell biology

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