Endothelial Cells: From Dysfunction Mechanism to Pharmacological Effect in Cardiovascular Disease

Haybar, Habib; Shahrabi, Saeid; Rezaeeyan, Hadi; Shirzad, Reza; Saki, Najmaldin

doi:10.1007/s12012-018-9493-8

Cited by 104 publications

(88 citation statements)

References 92 publications

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“…This is confirmed by the engagement of the CD40 receptor in endothelial cells that incites the production of adhesion molecules, cytokines, chemokines, and inflammatory mediators which are important for wound healing [33]. It has been widely reported in the literature that the binding of endothelial cell CD40 induces the expression of several proinflammatory cytokines as well as angiogenesis factors, including VEGF [12,34]. Our data on both fibroblasts and ECs confirm these findings, demonstrating both a direct proangiogenic effect on HUVEC (namely through an increase in the endogenous growth factor FGF-2) and indirect effect on fibroblasts (via the increase of VEGF) by Bioexpander ® .…”

Section: Discussionmentioning

confidence: 85%

“…Protein extraction and Western blot were performed as previously described [11][12][13]. Electrophoresis (50 µg of proteins/sample) was carried out in 4-12% Bis-Tris Gels (Life Technologies, Carlsbad, CA, USA).…”

Section: Western Blot For Protein Expressionmentioning

confidence: 99%

“…CD-40, a marker of EC activation toward an inflammatory and angiogenic phenotype [12,24,25], was unchanged after exposure of the HUVECs to Bioregen ® , while it mildly increased following incubation with Bioexpander ® (Figure 4).…”

Section: Ha Preparations Affect the Expression Of Angiogenesis Markermentioning

confidence: 99%

“…At tissue level, vascularization and organization of the stromal components are perquisites for tissue homeostasis and the biocompatibility of synthetic medical devices. An adequate blood supply guarantees cell survival and correct biochemical functioning without oedema formation, inflammatory cell infiltration, and scar development [11,12]. ECs are very sensitive to exogenous biomaterials and mechano-physical stimuli in general [13][14][15].…”

Section: Introductionmentioning

confidence: 99%

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Comparison of the Effect of Two Hyaluronic Acid Preparations on Fibroblast and Endothelial Cell Functions Related to Angiogenesis

Ciccone

Zazzetta²,

Morbidelli

2019

Cells

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Hyaluronic acid (HA) is used in substitutive and aesthetic medicine with various applications. Ultrapure absorbable HA (Bioregen ® ) and a mix of reticulated and free low molecular weight HA (Regenyal Idea Bioexpander ® ) (both provided by Regenyal Laboratories Srl, San Benedetto del Tronto (AP), Italy) represent a reliable hydrating device and skin filler, useful for skin blemishes, lines and wrinkles, and lip widening, respectively. The commercial products are known for their safety, but data on the molecular, cellular, and tissue responses are lacking. We aimed to evaluate the bioavailability and the pro-angiogenic features of the products Bioregen ® and Bioexpander ® in vitro on cultured endothelial cells (ECs) and dermal fibroblasts in vivo when injected into experimental animals. When added to fibroblasts and ECs, Bioexpander ® induced cell migration. The two HA preparations were well tolerated, while a transient proangiogenic behavior of Bioexpander ® , when implanted subcutaneously in mice, was found. The neovascular response was evident in the first week with higher levels of VEGF and FGF-2 before undergoing regression. In conclusion, our data strengthen the safety of HA synthetic preparations both in vitro and in vivo. Even if a proangiogenic response is documented, it is modest and transient, leading to tissue recovery and absence of an inflammatory infiltrate.

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Section: Discussionmentioning

confidence: 85%

Section: Western Blot For Protein Expressionmentioning

confidence: 99%

Section: Ha Preparations Affect the Expression Of Angiogenesis Markermentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Comparison of the Effect of Two Hyaluronic Acid Preparations on Fibroblast and Endothelial Cell Functions Related to Angiogenesis

Ciccone

Zazzetta²,

Morbidelli

2019

Cells

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“…Endothelial cell dysfunction and activation play major roles in the development and progression of CVD [182]. Zinc has shown extensive and potent antioxidant and anti-inflammatory properties [183].…”

Section: Zincmentioning

confidence: 99%

Trace Elements, PPARs, and Metabolic Syndrome

Shi

Zou

Shen

et al. 2020

IJMS

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Metabolic syndrome (MetS) is a constellation of metabolic derangements, including central obesity, insulin resistance, hypertension, glucose intolerance, and dyslipidemia. The pathogenesis of MetS has been intensively studied, and now many factors are recognized to contribute to the development of MetS. Among these, trace elements influence the structure of proteins, enzymes, and complex carbohydrates, and thus an imbalance in trace elements is an independent risk factor for MetS. The molecular link between trace elements and metabolic homeostasis has been established, and peroxisome proliferator-activated receptors (PPARs) have appeared as key regulators bridging these two elements. This is because on one hand, PPARs are actively involved in various metabolic processes, such as abdominal adiposity and insulin sensitivity, and on the other hand, PPARs sensitively respond to changes in trace elements. For example, an iron overload attenuates hepatic mRNA expression of Ppar-α; zinc supplementation is considered to recover the DNA-binding activity of PPAR-α, which is impaired in steatotic mouse liver; selenium administration downregulates mRNA expression of Ppar-γ, thereby improving lipid metabolism and oxidative status in the liver of high-fat diet (HFD)-fed mice. More importantly, PPARs' expression and activity are under the control of the circadian clock and show a robust 24 h rhythmicity, which might be the reasons for the side effects and the clinical limitations of trace elements targeting PPARs. Taken together, understanding the casual relationships among trace elements, PPARs' actions, and the pathogenesis of MetS is of great importance. Further studies are required to explore the chronopharmacological effects of trace elements on the diurnal oscillation of PPARs and the consequent development of MetS.

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Clinical implication of quantitative flow ratio to predict clinical events after drug‐coated balloon angioplasty in patients with in‐stent restenosis

Tang

Hou

Chu

et al. 2021

Clinical Cardiology

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Background The association between the quantitative flow ratio (QFR) and adverse events after drug‐coated balloon (DCB) angioplasty for in‐stent restenosis (ISR) lesions has not been investigated. Hypothesis Post‐procedural QFR is related to adverse events in patients undergoing DCB angioplasty for ISR lesions. Methods This retrospective study included data from patients undergoing DCB angioplasty for drug‐eluting stent (DES) ISR between January 2016 and February 2019. The QFR was measured at baseline and after DCB angioplasty. The endpoint was the vessel‐oriented composite endpoint (VOCE), defined as a composite of cardiac death, vessel‐related myocardial infarction, and ischemia‐driven target vessel revascularization. Results Overall, 177 patients with 185 DES‐ISR lesions were included. During 1‐year follow‐up, 27 VOCEs occurred in 26 patients. The area under curve (AUC) of the post‐procedural QFR was statistically greater than that of the in‐stent percent diameter stenosis (0.77, 95% confidence interval [CI] 0.67–0.87 vs. 0.64, 95% CI 0.53–0.75; p = .032). Final QFR cutoff of 0.94 has the best predictive accuracy for VOCE. A QFR > 0.94 was associated with a lower risk of VOCE compared to a QFR ≤ 0.94 (log‐rank test, p < .0001). Survival analysis using the multivariable Cox model showed that a post‐procedural QFR ≤ 0.94 was an independent predictor of 1‐year VOCE (hazard ratio 6.53, 95% CI 2.70–15.8, p < .001). Conclusions A lower QFR value was associated with worse clinical outcomes at 1 year after DCB angioplasty for DES‐ISR.

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Endothelial Cells: From Dysfunction Mechanism to Pharmacological Effect in Cardiovascular Disease

Cited by 104 publications

References 92 publications

Comparison of the Effect of Two Hyaluronic Acid Preparations on Fibroblast and Endothelial Cell Functions Related to Angiogenesis

Comparison of the Effect of Two Hyaluronic Acid Preparations on Fibroblast and Endothelial Cell Functions Related to Angiogenesis

Trace Elements, PPARs, and Metabolic Syndrome

Clinical implication of quantitative flow ratio to predict clinical events after drug‐coated balloon angioplasty in patients with in‐stent restenosis

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