Endothelial derived factors inhibit anoikis of head and neck cancer stem cells

Campos, Melina; Neiva, Kathleen G.; Meyers, K; Krishnamurthy, S

doi:10.1016/j.oraloncology.2011.09.010

Cited by 39 publications

(35 citation statements)

References 31 publications

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“…13,25,41,42 Our current data indicate that tumor cells grown as orospheres (a condition that favors CSC growth) were highly positive for sLeX compared to their adherent counterpart; even the sLeX- orospheres became 100% sLeX+ within few days. Floating orospheres were strongly positive for CD44 and ALDH confirming sLeX preferential expression in CSC.…”

Section: Discussionmentioning

confidence: 62%

In vitro Evaluation of Sialyl Lewis X Relationship with Head and Neck Cancer Stem Cells

Czerwinski

Desiderio

Shkeir

et al. 2013

Otolaryngol.--head neck surg.

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Objective To evaluate in vitro the potential links between sialyl Lewis X (sLeX) and cancer stem cells (CSC) in head and neck squamous cell carcinoma (HNSCC). HNSCC is an aggressive malignancy with high mortality mainly due to metastasis. CSC have emerged as important players in HNSCC metastasis. sLeX is a tetrasaccharide carbohydrate known to play a key-role in metastatic dissemination by promoting binding of the tumor cells to the endothelium. Study Design Experimental, in vitro. Setting Laboratory of Head and Neck Cancer Metastasis, University of Michigan. Subjects and Methods A panel of stage- and anatomic-site specific primary and metastatic HNSCC cell lines was assessed by flow cytometry to quantify sLeX relative expression levels. Serum-free conditioned media from the same HNSCC lines was collected over a time-course of 72 hours and assessed by Western-blot for secreted sLeX expression. Representative HNSCC cell lines were cultured as floating orospheres (condition that enhance CSC growth) or under normal adherent conditions and characterized by flow cytometry for CSC markers (CD44, aldehyde dehydrogenase [ALDH]) comparatively with sLeX expression. Results sLeX is predominantly expressed in carcinomas originating from the oral cavity. Secreted sLeX is also found to be high in oral carcinomas and increased over the analyzed time course. Floating orospheres were strongly positive for CD44 and ALDH confirming CSC enrichment of the orospheres. Tumor cells grown as orospheres are 95-100% positive for sLeX compared to 10-40% of adherent counterpart. Conclusion These studies provide the first evidence of sLeX relationship with CSC in HNSCC.

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Section: Discussionmentioning

confidence: 62%

In vitro Evaluation of Sialyl Lewis X Relationship with Head and Neck Cancer Stem Cells

Czerwinski

Desiderio

Shkeir

et al. 2013

Otolaryngol.--head neck surg.

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“…CSCs reside in close proximity to blood vessels and can give rise to tumor endothelium (24). It was reported that endothelialderived factors inhibit anoikis of ALDH + CD44 + head and neck cancer stem cells (25). EMT is regarded a necessary process that empowers CSCs to disseminate from primary tumors and to seed metastases.…”

Section: Discussionmentioning

confidence: 99%

ALDH1-positive cancer stem-like cells are enriched in nodal metastases of oropharyngeal squamous cell carcinoma independent of HPV status

Qian

Wagner

et al. 2013

Oncology Reports

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Abstract. Oropharyngeal squamous cell carcinoma (OSCC) is caused by high-risk (HR) human papillomavirus (HPV) or alcohol and tobacco abuse. Aldehyde dehydrogenase 1 (ALDH1) is a confirmed marker for cancer stem-like cells (CSCs) of OSCC responsible for therapy resistance, recurrence and metastasis. Associations between HR-HPV/p16, CSC frequency and clinicopathological parameters in patients with metastatic OSCC were investigated. In the present study, HPV genotypes and expression of ALDH1 and p16 was analyzed in 40 paired OSCC and metastases. A significant correlation between ALDH1 positivity with lower primary tumor differentiation grade (P=0.009) and higher nodal status (P=0.015) was noted. Compared to primary tumors, the proportion of ALDH1-expressing cells was significantly increased in metastases (P=0.012), while significantly fewer ALDH1-expressing cells were found in HR-HPV-DNA + /p16 + primary tumors (P=0.038) compared to HR-HPV-DNA -/p16 -primary tumors. Metastases showed no difference. ALDH1 + CSCs are detectable in OSCC and metastases. ALDH1 high-grade OSCC exhibits a more aggressive phenotype characterized by higher nodal classification and lower differentiation. This suggests a subpopulation contained in the ALDH1-positive OSCC cell pool able to complete the metastatic cascade and subsequently enriching in metastasis independent of tumor etiology and ALDH1 content.

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“…We have recently reported that UM-SCC-22B contains a sub-population of cells that exhibit cancer stem-like characteristics. 160 Photomicrographs (200x) of UM-SCC-22B cells cultured with serum-free medium in ultra-low attachment plates in presence of 0 or 50 ng/ml EGF for 5 days. The formation of spheroid-like colonies containing at least 25 cells (named orospheres) was enhanced by EGF treatment.…”

Section: Figurementioning

confidence: 99%

The biology of head and neck cancer stem cells

Zhang¹,

Filho²,

Nör³

2012

Oral Oncology

Self Cite

152

134

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Emerging evidence indicates that a small population of cancer cells is highly tumorigenic, endowed with self-renewal, and has the ability to differentiate into cells that constitute the bulk of tumors. These cells are considered the “drivers” of the tumorigenic process in some tumor types, and have been named cancer stem cells. Epithelial-mesenchymal transition (EMT) appears to be involved in the process leading to the acquisition of stemness by epithelial tumor cells. Through this process, cells acquire an invasive phenotype that may contribute to tumor recurrence and metastasis. Cancer stem cells have been identified in human head and neck squamous cell carcinomas (HNSCC) using markers such as CD133 and CD44 expression, and aldehyde dehydrogenase (ALDH) activity. The head and neck cancer stem cells reside primarily in perivascular niches in the invasive front where endothelial-cell initiated events contribute to their survival and function. In this review, we discuss the state-of-the-knowledge on the pathobiology of cancer stem cells, with a focus on the impact of these cells to head and neck tumor progression.

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Endothelial derived factors inhibit anoikis of head and neck cancer stem cells

Cited by 39 publications

References 31 publications

In vitro Evaluation of Sialyl Lewis X Relationship with Head and Neck Cancer Stem Cells

In vitro Evaluation of Sialyl Lewis X Relationship with Head and Neck Cancer Stem Cells

ALDH1-positive cancer stem-like cells are enriched in nodal metastases of oropharyngeal squamous cell carcinoma independent of HPV status

The biology of head and neck cancer stem cells

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