Endothelial Differentiated Adipose-Derived Stem Cells Improvement of Survival and Neovascularization in Fat Transplantation

Harris, William M.; Plastini, Michael; Kappy, Nikolas; Ortiz, Telisha; Chang, Shaohua; Brown, Spencer A.; Carpenter, Jeffrey P.; Zhang, Ping

doi:10.1093/asj/sjy130

Cited by 33 publications

(23 citation statements)

References 38 publications

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“…A key challenge in regenerative medicine is tissue minimal manipulation, and we hope that this study may open the door to possible optimization processes associated with fat grafting operative procedures [47].…”

Section: Discussionmentioning

confidence: 99%

Long-Lasting Anti-Inflammatory Activity of Human Microfragmented Adipose Tissue

Nava

Sordi

Pascucci

et al. 2019

Stem Cells International

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Over the last few years, human microfragmented adipose tissue (MFAT), containing significant levels of mesenchymal stromal cells (MSCs) and obtained from fat lipoaspirate (LP) through a minimal manipulation in a closed system device, has been successfully used in aesthetic medicine as well as in orthopedic and general surgery. Interestingly, in orthopedic diseases, this ready-to-use adipose tissue cell derivative seems to have a prolonged time efficacy even upon a single shot injection into osteoarthritic tissues. Here, we investigated the long-term survival and content of MSCs as well the anti-inflammatory activity of LP and its derived MFAT in vitro, with the aim to better understand a possible in vivo mechanism of action. MFAT and LP specimens from 17 human donors were investigated side by side. During a long-term culture in serum-free medium, we found that the total cell number as well the MSC content in MFAT decreased more slowly if compared to those from LP specimens. The analysis of cytokines and growth factors secreted into the conditioned medium (CM) was similar in MFAT and LP during the first week of culture, but the total amount of cytokines secreted by LP decreased much more rapidly than those produced by MFAT during prolonged culture (up to 28 days). Similarly, the addition of MFAT-CM recovered at early (3-7 days) and late stage (14-28 days) of culture strongly inhibited inflammatory function of U937 monocyte cell line, whereas the anti-inflammatory activity of LP-CM was drastically reduced after only 7 days of culture. We conclude that MFAT is an effective preparation with a long-lasting anti-inflammatory activity probably mediated by a long-term survival of their MSC content that releases a combination of cytokines that affect several mechanisms involved in inflammation processes.

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Section: Discussionmentioning

confidence: 99%

Long-Lasting Anti-Inflammatory Activity of Human Microfragmented Adipose Tissue

Nava

Sordi

Pascucci

et al. 2019

Stem Cells International

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“…Preclinical and clinical settings of ASC showed auspicious therapies, showing immunotolerant properties and easy isolation. As autologous fat transplantation is restricted by low vascularization, fat absorption and fibrosis, co-transplantation of ASC-derived extracellular vesicles with fat tissue into a mouse model increased tissue volume retention due to vascularization enhancement and inflammatory response [164,165]. The paracrine products of ASCs, analyzed by loading them into a microencapsulation device, enhanced the angiogenic activity of endothelial cells and influenced macrophages secretion.…”

Section: Allograftmentioning

confidence: 99%

The Proliferation and Differentiation of Adipose-Derived Stem Cells in Neovascularization and Angiogenesis

Hutchings

Janowicz

Moncrieff

et al. 2020

IJMS

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Neovascularization and angiogenesis are vital processes in the repair of damaged tissue, creating new blood vessel networks and increasing oxygen and nutrient supply for regeneration. The importance of Adipose-derived Mesenchymal Stem Cells (ASCs) contained in the adipose tissue surrounding blood vessel networks to these processes remains unknown and the exact mechanisms responsible for directing adipogenic cell fate remain to be discovered. As adipose tissue contains a heterogenous population of partially differentiated cells of adipocyte lineage; tissue repair, angiogenesis and neovascularization may be closely linked to the function of ASCs in a complex relationship. This review aims to investigate the link between ASCs and angiogenesis/neovascularization, with references to current studies. The molecular mechanisms of these processes, as well as ASC differentiation and proliferation are described in detail. ASCs may differentiate into endothelial cells during neovascularization; however, recent clinical trials have suggested that ASCs may also stimulate angiogenesis and neovascularization indirectly through the release of paracrine factors.

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“…Increasing evidence suggests that ADSCs with other types of MSCs, are anatomically and functionally associated with vascular and perivascular habitats in various tissues. Harris et al confirmed that the co-implantation of fat lipoaspirate with ADSCs differentiated toward an endothelial phenotype observably improved both survival and neovascularization of the transplanted fat lipoaspirate 74 . An interesting study demonstrated that CD146 positive cells selected from a heterogeneous mixture of ADSCs expressed higher levels of the adipocyte markers adiponectin and leptin, and possessed more favorable angiogenic and adipogenic properties, which might provide significant benefits for reconstructive and tissue-engineering applications in fat transplantation 75 .…”

Section: Adscs Angiogenesismentioning

confidence: 92%

Deciphering the Emerging Roles of Adipocytes and Adipose-Derived Stem Cells in Fat Transplantation

Zhao

et al. 2021

Cell Transplant

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Autologous fat transplantation is widely regarded as an increasingly popular method for augmentation or reshaping applications in soft tissue defects. Although the fat transplantation is of simple applicability, low donor site morbidity and excellent biocompatibility, the clinical unpredictability and high resorption rates of the fat grafts remain an inevitable problem. In the sites of fat transplantation, the most essential components are the adipocyte and adipose-derived stem cells (ADSCs). The survival of adipocytes is the direct factor determining fat retention. The efficacy of fat transplantation is reduced by fat absorption and fibrosis due to the inadequate blood flow, adipocyte apoptosis and fat necrosis. ADSCs, a heterogeneous mixture of cells in adipose tissue, are closely related to tissue survival. ADSCs exhibit the ability of multilineage differentiation and remarkable paracrine activity, which is crucial for graft survival. This article will review the recent existing research on the mechanisms of adipocytes and ADSCs in fat transplantation, especially including adipocyte apoptosis, mature adipocyte dedifferentiation, adipocyte browning, ADSCs adipogenic differentiation and ADSCs angiogenesis. The in-depth understanding of the survival mechanism will be extremely valuable for achieving the desired filling effects.

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Endothelial Differentiated Adipose-Derived Stem Cells Improvement of Survival and Neovascularization in Fat Transplantation

Cited by 33 publications

References 38 publications

Long-Lasting Anti-Inflammatory Activity of Human Microfragmented Adipose Tissue

Long-Lasting Anti-Inflammatory Activity of Human Microfragmented Adipose Tissue

The Proliferation and Differentiation of Adipose-Derived Stem Cells in Neovascularization and Angiogenesis

Deciphering the Emerging Roles of Adipocytes and Adipose-Derived Stem Cells in Fat Transplantation

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