Endothelial nitric oxide synthase Glu298Asp gene polymorphism influences body composition and biochemical parameters but not the nitric oxide response to eccentric resistance exercise in elderly obese women

Abstract

Both endothelial nitric oxide synthase (eNOS) gene polymorphism and nitric oxide (NO) are involved in important cardiovascular, muscular and inflammatory physiological mechanisms during ageing and response to exercise. The aim of this study was to investigate the NO kinetic response following an acute eccentric resistance exercise (ERE) session and the possible effect of the Glu298Asp eNOS gene polymorphism in elderly obese women. Eighty-seven women (age 69·4 ± 6·1 years, body weight 74·9 ± 12·7 kg, height 151·9 ± 6·0 cm and BMI 32·5 ± 5·7 kg m ) completed seven sets of ten eccentric repetitions at 110% of the ten repetitions maximum (10RM). NO concentrations remained elevated up to 48 h following the acute ERE session as compared with baseline, for GG and GT/TT groups (P<0·05), with no differences between genotypes. The GG genotype group had higher body weight, prevalence of obesity (BMI classification - 81% versus 56%), BMI and higher relative muscle strength, while they had significantly lower triglycerides, VLDL and urea concentrations as compared with TT/TG group. In conclusion, NO remains elevated for up to 48 h after an acute ERE session, without genotype interaction. The TT/TG genotype had a negative impact on triglycerides, VLDL and urea concentrations. Thus, T carriers should increase their attention to cardiovascular risk factor and metabolic disorders.

Understanding responsiveness of nitric oxide to acute eccentric resistance exercise in elderly obese women

“…We certainly agree that our definition of responsiveness was specific to our study population and exercise type (ERE). Limitations in this study such as genetic variation, the use of one exercise as compared with seven exercises in another study [19], absence of a non-obese group and the lack of a specific diet control as the timing and composition of dietary intake can modulate training responses [28]. In addition, we do not know if the exercise stimulus would have been low enough in the small group of LR to induce a CK and NO release.…”

“…Furthermore, the CK reference values for women are 40 to 150 U/l [14). Although the magnitude of CK activity after exercise was small, when accompanied with NO increments after exercise in the HR subjects, it could be indicative of muscle damage and regeneration [28]. The greater NO response following an ERE session and the greater CK response in the HR subjects might infer a better adaptive response to injury and regeneration in these individuals.…”

Influência De Polimorfismos Genéticos Na Expressão De Enos, Apoe E Eca Em Resposta Ao Treinamento De Força Excêntrico Em Idosas Obesas.

“…Além disso, Tajra et al, (2014) também observaram em idosas obesas, aumento na CK e IL-6 após 48hrs e 24hrs após sessão de TFE, porém tais níveis foram considerados normais e não indicativos de excesso de dano muscular assim como no presente estudo. E estudo de Teixeira et al, (2015), indicando que o protocolo de TFE utilizado é suficiente para induzir dano muscular pela mediado através da CK, mas sem uma resposta exacerbada.…”

“…Por fim, foram selecionados apenas 3 artigos que se encaixavam nos critérios de inclusão. Destes, apenas um tratava sobre a respostas do polimorfismo do gene em eNOS ao exercício excêntrico em idosas (TEIXEIRA et al, 2015), dois sobre treinamento resisto e as influências do polimorfismo genético de ECA (MOTA et al, 2013;PEREIRA et al, 2013), e nenhum trazia informações sobre polimorfismos de APOE e treinamento.…”

Haplotype analysis of endothelial nitric oxide synthase (NOS3) genetic variants and metabolic syndrome in healthy subjects and schizophrenia patients

“…We revealed no associations for G894T or C774T SNPs with biochemical parameters of lipid and carbohydrate metabolism in MetS patients with or without SCH. However, some other studies demonstrated the associations between G894T SNP and plasma triglycerides in Caucasian patients with obesity and T2DM [ 49 , 50 ]. Moreover, NOS3 G894T polymorphism is likely a predictor for persistent hyperglycemia for individuals who have a compromised glucose tolerance and atherogenic lipid profile in Asian populations [ 30 , 46 , 51 ].…”