Endothelial nitric oxide synthase/soluble guanylate cyclase system in human nasal polyps

Abstract

The aim of our study was to analyze the level of expression of the endothelial nitric oxide synthase (eNOS)/soluble guanylate cyclase (sGC) system in nasal polyps and control nasal mucosae. The study was performed in polyps from 15 patients and nasal mucosae from 11 subjects operated on the nasal septum (control group). The expression of endothelial nitric oxide synthase (eNOS) and soluble guanylate cyclase (sGC) was determined in nasal mucosae. Western blot analysis demonstrated that eNOS protein was overexpressed in the nasal polyps with respect to control nasal mucosae. Immunohistochemistry also demonstrated that the vascular endothelium of nasal polyps contained higher amounts of eNOS protein than control nasal mucosae. Moreover, the beta(1) subunit of sGC was also overexpressed in the nasal polyps, which was associated with an increased content of cyclic GMP in the nasal polyps with respect to nasal control mucosae. In human nasal polyposis, there is an overexpression of the eNOS/sGC system. Further studies are needed to assess whether this overexpression is involved in the genesis of nasal polyposis.

Asymmetric Dimethylarginine Levels in Allergic Rhinitis and Nasal Polyposis

“…An increase in oxidative stress and deficient antioxidant mechanisms may also play a role in the pathophysiology of NP (26,27). Several studies have reported increased NOS levels in NP patients (28,29). We also found lower ADMA values in the NP group than in the control group, but there was no statistically significant difference.…”