Endothelial progenitor cells, atheroma burden and clinical outcome in patients with coronary artery disease

Abstract

Traditional EPC populations, CD34(+)VEGFR-2(+) and CD34(+)VEGFR-2(+)CD133(+) are not related to the extent of CAD or clinical outcome. However, CD34(+)CD45(-) cells are increased in patients with CAD and predict future cardiovascular events. It is likely that CD34(+)CD45(-) concentrations reflect the extent of vascular injury and atheroma burden.

Large platelets but not putative endothelial progenitor cells are associated with low strut coverage after drug-eluting stent implantation

“…Concerning the investigated populations of putative EPC and CEC, all subgroups have been quantified in similar patient populations [7,[27][28][29]. Compared with these reports, our EPC and CEC numbers were highly similar.…”

“…Yet, another group has reported no increase of CD34 + cells after implantation of sirolimus-eluting stents measuring at various time points up to one month [35]. Thus, our model of assessing strut coverage 6 months after stent implantation should well reflect the influence of EPC on late vascular healing as it has been proposed more recently [28]. Early analysis of stent strut coverage in patients with acute coronary syndromes might represent an alternative to assess the effects of high CEC or EPC levels, yet the presence of thrombotic material may hinder identification of true endothelium [12,14].…”

“…S2, Table S2) [7,[27][28][29]. However, assessment of viability by absence of 7-AAD staining in the lymphocyte gate ( Fig.…”

Analysis of Various Subsets of Circulating Mononuclear Cells in Asymptomatic Coronary Artery Disease

“…The authors agree with Padfield G. J. et al . [15] who showed that CD34 + CD45 − cell number was increased in patients with CAD compared with those with normal coronary arteries and correlated well with atheroma. The authors believed that CD14 + VEGFR-2 + Tie-2 + cells and endothelial cell-colony forming units were increased in patients with acute coronary syndrome; that cell concentrations reflected myocardial necrosis, and did not predict the extent of CAD.…”

“…Basically, CD34 + CD45 − cells have nonhematopoietic origin; and recently they have been identified as putative EPCs by virtue of their ability to form “late outgrowth” colonies phenotypically and functionally indistinguishable from mature endothelial cell colonies in culture [12,13]. Previous studies have shown an increase in the circulating concentration of population CD34 + CD45 − cells in patients with established coronary artery disease (CAD) and peripheral artery disease [10,14,15]. Theoretically, CD34 + cells which do not express CD45 by flow cytometry may include cells capable of developing bona fide endothelial cells.…”