Endothelial Protein C Receptor Could Contribute to Experimental Malaria-Associated Acute Respiratory Distress Syndrome

Ortolan, Luana dos Santos; Sercundes, Michelle Klein; Moura, Gabriel Cândido; Quirino, Thatyane de Castro; Debone, Daniela; Costa, Douglas de Sousa; Murillo, Oscar; Marinho, Cláudio Romero Farias; Epiphânio, Sabrina

doi:10.1155/2019/3105817

Cited by 7 publications

(16 citation statements)

References 66 publications

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“…Authors suggested that high levels of sEPCR in these group could be secondary to TNF-alpha associated EPCR dysfunction [ 24 ]. Similar findings were reported in two different studies evaluating septic shock patients secondary to pneumococcal pneumonia and experimental malaria-associated ARDS model [ 21 , 22 ]. These findings support the view that there is an intersection between coagulation and inflammation cascade in systemic infectious diseases with severe lung involvement and that protein C system located at this junction.…”

Section: Discussionsupporting

confidence: 90%

Relationship between serum soluble endothelial protein C receptor level and COVID-19 findings

Bayrakci

Özkan²,

Mutlu³

et al. 2021

Blood Coagulation &Amp; Fibrinolysis

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Coronavirus-related disease-2019 (COVID-19)-associated coagulopathy presents predominantly with thrombosis and leads to complications in close association with inflammatory process. Soluble endothelial protein C receptor (sEPCR), which is the soluble form of EPCR, reduces the anticoagulant and anti-inflammatory activity of activated protein C. The purpose of this study is to investigate the relationship between sEPCR and the laboratory parameters and thorax computed tomography (CT) findings in the course of COVID-19. Twenty-five laboratory-confirmed [reverse transcription-quantitative polimerase chain reaction (RT-qPCR) positive] and 24 clinically diagnosed (RT-qPCR negative) COVID-19 patients were enrolled in the study. Blood specimens were collected for sEPCR and haematological and biochemical parameter measurement. Thorax CT was performed to detect COVID-19 findings. These parameters from RT-qPCR positive and negative patients were then compared. Although there was no difference between the groups in terms of symptoms, the time between the onset of symptoms and the admission time was shorter in RT-qPCR positive group ( P = 0.000). sEPCR levels were significantly higher in the RT-qPCR positive group ( P = 0.011). Patients with ground-glass opacity and bilateral involvement on thorax CT have higher serum sEPCR levels ( P = 0.012 and 0.043, respectively). This study has shown for the first time that serum sEPCR levels, which is a member of coagulation cascade and has also been reported to be associated with inflammation, is higher in patients with positive RT-qPCR test and patients with GGO or bilateral involvement on thorax CT regardless of the PCR result.

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Section: Discussionsupporting

confidence: 90%

Relationship between serum soluble endothelial protein C receptor level and COVID-19 findings

Bayrakci

Özkan²,

Mutlu³

et al. 2021

Blood Coagulation &Amp; Fibrinolysis

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“…These findings are compatible with our results since the ARDS-developing mice present an increase in vascular permeability evidenced by pleural effusion reaching 1 ml of fluid in the thoracic cavity, which would complicate compliance. Our previous results showed increased pulmonary vascular permeability in malaria infection in DBA/2 model both in vivo and in vitro [4,5,38,39]. In addition, we observed that, after 14 th dpi, an improvement in lung aeration when compared to that on the 7 th dpi.…”

Section: Plos Onesupporting

confidence: 50%

“…Mortality was monitored from the 5 th to 17 th day post infection (dpi). The experimental time course to was reduced to 17 days after infection, compared with other experiments of our model previously published [5,[37][38][39] to shorten any discomfort, pain or suffering that Plasmodium berghei infection may cause.…”

Section: Plasmodium Berghei Anka Infection Parasitemia and Euthanasiamentioning

confidence: 93%

“…Mice respiratory parameters (respiratory rate, enhanced pause (Penh), inspiratory and expiratory time, ventilation volume and tidal volume) were collected and quantified using unrestrained whole-body plestimography chambers (Buxco Eletronics, Harvard, USA) and FinePointe software, as previously described [39] on the 5 th and 7 th dpi.…”

Section: Respiratory Parameters Quantificationmentioning

confidence: 99%

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Lung aeration in experimental malaria-associated acute respiratory distress syndrome by SPECT/CT analysis

et al. 2020

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Malaria-associated acute respiratory distress syndrome (ARDS) is an inflammatory disease causing alveolar-pulmonary barrier lesion and increased vascular permeability characterized by severe hypoxemia. Computed tomography (CT), among other imaging techniques, allows the morphological and quantitative identification of lung lesions during ARDS. This study aims to identify the onset of malaria-associated ARDS development in an experimental model by imaging diagnosis. Our results demonstrated that ARDS-developing mice presented decreased gaseous exchange and pulmonary insufficiency, as shown by the SPECT/CT technique. The pulmonary aeration disturbance in ARDS-developing mice on the 5 th day post infection was characterized by aerated tissues decrease and nonaerated tissue accumulation, demonstrating increased vascular permeability and pleural effusion. The SPECT/CT technique allowed the early diagnosis in the experimental model, as well as the identification of the pulmonary aeration. Notwithstanding, despite the fact that this study contributes to better understand lung lesions during malaria-associated ARDS, further imaging studies are needed.

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“…Protein C has also been found to be an independent predictor of mortality for ALI/ARDS [ 130 , 208 ]. Mice with malaria-associated ARDS showed an increase in EPCR concentrations in lung homogenates [ 209 ], whereas loss of EPCR and TM was seen in lung specimens from patients who died from severe falciparum malaria with coexisting ARDS [ 210 ].…”

Section: Pulmonary Endothelial Functionsmentioning

confidence: 99%

Endothelial Damage in Acute Respiratory Distress Syndrome

Vassiliou

Κοτανίδου

Dimopoulou

et al. 2020

IJMS

162

104

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The pulmonary endothelium is a metabolically active continuous monolayer of squamous endothelial cells that internally lines blood vessels and mediates key processes involved in lung homoeostasis. Many of these processes are disrupted in acute respiratory distress syndrome (ARDS), which is marked among others by diffuse endothelial injury, intense activation of the coagulation system and increased capillary permeability. Most commonly occurring in the setting of sepsis, ARDS is a devastating illness, associated with increased morbidity and mortality and no effective pharmacological treatment. Endothelial cell damage has an important role in the pathogenesis of ARDS and several biomarkers of endothelial damage have been tested in determining prognosis. By further understanding the endothelial pathobiology, development of endothelial-specific therapeutics might arise. In this review, we will discuss the underlying pathology of endothelial dysfunction leading to ARDS and emerging therapies. Furthermore, we will present a brief overview demonstrating that endotheliopathy is an important feature of hospitalised patients with coronavirus disease-19 (COVID-19).

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Endothelial Protein C Receptor Could Contribute to Experimental Malaria-Associated Acute Respiratory Distress Syndrome

Cited by 7 publications

References 66 publications

Relationship between serum soluble endothelial protein C receptor level and COVID-19 findings

Relationship between serum soluble endothelial protein C receptor level and COVID-19 findings

Lung aeration in experimental malaria-associated acute respiratory distress syndrome by SPECT/CT analysis

Endothelial Damage in Acute Respiratory Distress Syndrome

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