Endothelial pyroptosis underlies systemic inflammatory response following radiofrequency ablation of hepatic hemangiomas

Wang, Shaohong; Yang, Xu; Xu, Li; Shi, Ke; Ding, Xuemei; Sun, Wenbing; Gao, Jun

doi:10.1080/00365513.2019.1689428

Cited by 22 publications

(27 citation statements)

References 21 publications

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“…Clinically, high glucose activates pyroptosis through the caspase1/GSDMD/IL-1β pathway to release proinflammatory cytokines, repressing the proliferation and differentiation of osteoblast in alveolar bone, and Ac-YVAD-CMK, a caspase1 inhibitor, can reverse expression of these proteins and attenuate the formation of periodontal diseases [62]. The expression of caspase1, GSDMD, IL-18 and IL-1β increase significantly in the endothelial cells of subablated hepatic hemangioma in thirty-two patients after radiofrequency ablation, and the caspase-1-associated endothelial pyroptosis is induced by subablative hyperthermia and Ac-YVAD-CMK attenuates pyroptosis in vitro experiments [70].…”

Section: Compounds Inhibiting Pyroptosis To Treat Inflammatory Diseasesmentioning

confidence: 99%

Mechanisms and Therapeutic Regulation of Pyroptosis in Inflammatory Diseases and Cancer

Zheng

2020

IJMS

147

111

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Programmed Cell Death (PCD) is considered to be a pathological form of cell death when mediated by an intracellular program and it balances cell death with survival of normal cells. Pyroptosis, a type of PCD, is induced by the inflammatory caspase cleavage of gasdermin D (GSDMD) and apoptotic caspase cleavage of gasdermin E (GSDME). This review aims to summarize the latest molecular mechanisms about pyroptosis mediated by pore-forming GSDMD and GSDME proteins that permeabilize plasma and mitochondrial membrane activating pyroptosis and apoptosis. We also discuss the potentiality of pyroptosis as a therapeutic target in human diseases. Blockade of pyroptosis by compounds can treat inflammatory disease and pyroptosis activation contributes to cancer therapy.

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Section: Compounds Inhibiting Pyroptosis To Treat Inflammatory Diseasesmentioning

confidence: 99%

Mechanisms and Therapeutic Regulation of Pyroptosis in Inflammatory Diseases and Cancer

Zheng

2020

IJMS

147

111

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“…The most important function of cleaved caspase-1 is to facilitate GSDMD cleavage into an N-terminal effector domain and a C-terminal inhibitory domain. The N-terminal domain can bind to PIPs in the cell membrane and promote the release of intracellular in ammatory substances, such as IL-1β, through pore opening in cell membrane, ultimately leading to pyroptosis [33]. In agreement with the previous reports, this research results con rmed that caspase-1 and GSDMD protein expression levels, determined by ELISA, immuno uorescence and western blot, were increased in HLE-B3 hLECs at a does-dependent manner, as.…”

Section: Discussionmentioning

confidence: 99%

Short-Wavelength Blue Light Contributes to Pyroptosis of Human Lens Epithelial Cells (hLECs) by Caspase-1-GSDMD Signalling Axis Activation

Song¹,

Wang²,

Li³

et al. 2020

Preprint

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Backgroud: To examine the effects of short-wavelength blue light (SWBL) on cultured human lens epithelial cells (hLECs). The nosogenesis of cataracts after SWBL exposure was discussed. Methods: HLE-B3 hLECs were divided into 3 groups randomly: A: normal control group, which consisted of hLECs cultured in the dark; B: the caspase-1 inhibitor group; and C: the SWBL exposure group. After the SWBL (2500 lux) irradiation (for 8, 16, 24, and 32 h), the caspase-1 and gasdermin D (GSDMD) expression levels in HLE-B3 hLECs were examined using ELISA, immunofluorescence, and Western blotting analyses. Double-positive staining of HLE-B3 hLECs for activated and inhibited caspase-1 was used to confirm pyroptosis in hLECs by flow cytometry. Results: SWBL can cause cell death in HLE-B3 hLECs, but a caspase-1 inhibitor suppressed cell death. The flow cytometry results also confirmed the does-dependent of short-wavelength blue light irradiation on pyroptotic death of hLECs. Caspase-1 and GSDMD expression levels of all hLECs groups changed with short-wavelength blue light exposure times (8, 16, 24, and 32 h) and were higher in groups B and C than group A. The immunofluorescence results demonstrated that the expression of GSDMD-N was higher in the cell membrane in both the B and C groups than in the A group.Conclusion: The data indicate that SWBL induces pyroptotic programmed cell death by activation of the GSDMD signalling axis in HLE-B3 hLECs. These results provide new insights into the exploitation of new candidates for the prevention of cataracts.

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“…Tang and colleagues compared different surgical techniques, both laparotomic and laparoscopic, with percutaneous MWA [19][20][21][22].…”

Section: Ablation Proceduresmentioning

confidence: 99%

“…While no technical failure was reported among the studies evaluated, procedure effectiveness rate varied with the specific ablation technique considered. Li and Wang, which used MW energy under US guidance, reported a rate of complete tumoral ablation of 95.9% and 84.6%, respectively [19,23]. In the study by Liu F and colleagues the mean tumoral volume shrinkage rate was 59.67% within three days after US-guided MW ablation [16].…”

Section: Procedural and Clinical Outcomesmentioning

confidence: 99%

“…Wang and Cheng described the development of post-ablation systemic inflammatory response (SIR) in 50% and 75.7% of patients, respectively, within two days after intervention [14,19]. Wang and colleagues reported low fever, constipation, slight wound pain, stomach discomfort and other MWA minor postoperative reactions in 11 out of 12 treated patients [15].…”

Section: Complicationsmentioning

confidence: 99%

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Current concepts in ablative procedures for primary benign liver lesions: a step forward to minimize the invasiveness of treatment when deemed necessary

et al. 2020

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With increased use of medical imaging, the incidental detection of benign solid liver tumors has become more frequent. Facing with benign disease, the indications for surgery are still object of discussion in light of the stable natural course of most lesions and obvious drawbacks of any surgical intervention; therefore, in most situations, a conservative approach is recommended, and surgery is mainly reserved for those cases with persistent or worsening symptoms, or who are at risk for complications as malignant transformation. The advent of ablative techniques has widened the range of treatment options available to these patients, presenting as a valid alternative to resection in terms of safety and efficacy in selected cases, particularly in patients who are considered poor surgical candidates and with smaller lesions. This review outlines the role of percutaneous ablative methods for benign solid liver tumors that are encountered in adults, providing a per histology analysis of the existing evidence. The up-to-date strategies for management of the most common benign solid tumors are recapitulated.

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Endothelial pyroptosis underlies systemic inflammatory response following radiofrequency ablation of hepatic hemangiomas

Cited by 22 publications

References 21 publications

Mechanisms and Therapeutic Regulation of Pyroptosis in Inflammatory Diseases and Cancer

Mechanisms and Therapeutic Regulation of Pyroptosis in Inflammatory Diseases and Cancer

Short-Wavelength Blue Light Contributes to Pyroptosis of Human Lens Epithelial Cells (hLECs) by Caspase-1-GSDMD Signalling Axis Activation

Current concepts in ablative procedures for primary benign liver lesions: a step forward to minimize the invasiveness of treatment when deemed necessary

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