Endothelin-1 and endothelin receptors in the basilar artery of the capybara

Loesch, Andrzej; Gajkowska, B; Dashwood, Michael R.; Fioretto, Emerson Ticona; Gagliardo, Karina Martinez; Lima, Ana Rita de; Ribeiro, Antônio Augusto Coppi Maciel

doi:10.1007/s10735-004-2912-0

Cited by 9 publications

(20 citation statements)

References 41 publications

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“…According to the present study, the reverse is true in RPMVEC where the effects of microinjected ET-1 are mediated through intracellular ET B receptors. Our findings are particularly relevant considering the ability of endothelial cells to synthesize ET-1 (40 -42) and make it available within the cytoplasm (32,33,40) to activate its intracellular targets. Importantly, a majority of the responses elicited by ET-1 in the endothelium, including the release of vasorelaxant factors such as NO, prostacyclin, and endothelium-derived hyperpolarizing factor are ET B receptor-dependent (11).…”

Section: Discussionmentioning

confidence: 89%

Intracellular Endothelin Type B Receptor-driven Ca2+ Signal Elicits Nitric Oxide Production in Endothelial Cells

Deliu

Brailoiu

Mallilankaraman

et al. 2012

Journal of Biological Chemistry

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Background:The intracrine nature of endothelin-1 is largely correlated with nuclear signaling events. Results: In endothelial cells, endothelin-1 acting on endolysosomal ET B receptors increases cytosolic Ca 2ϩ and nitric oxide. Conclusion: Endolysosomal ET B receptors are functional. Significance: We identify a new pathway for ET-1-induced intracrine signaling and provide the first evidence that intracellular G protein-coupled receptors are involved in redox signaling.

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Section: Discussionmentioning

confidence: 89%

Intracellular Endothelin Type B Receptor-driven Ca2+ Signal Elicits Nitric Oxide Production in Endothelial Cells

Deliu

Brailoiu

Mallilankaraman

et al. 2012

Journal of Biological Chemistry

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“…Immunohistochemical analysis of the capybara basilar artery provided evidence of adventitial ET B receptors (24). However, results from an earlier study (19) suggested that ET B receptors were not present in the adventitia derived from a tissue-engineered human blood vessel.…”

Section: Discussionmentioning

confidence: 91%

ET_A and ET_B receptors are expressed in vascular adventitial fibroblasts

Boyd

Rätsep

Ding

et al. 2011

American Journal of Physiology-Heart and Circulatory Physiology

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The adventitia has been recognized to play important roles in vascular oxidative stress, remodeling, and contraction. We recently demonstrated that adventitial fibroblasts are able to express endothelin (ET)-1 in response to ANG II. However, it is unclear whether ET-1 receptors are expressed in the adventitia. We therefore investigated the expression and roles of both ETA and ETB receptors in collagen synthesis and ET-1 clearance in adventitial fibroblasts. Adventitial fibroblasts were isolated and cultured from the mouse thoracic aorta by the explant method. Cultured cells were treated with ANG II (100 nmol/l) or ET-1 (10 pM) in the presence or absence of the ANG II type 1 receptor antagonist losartan (100 M), the ET-1 receptor antagonists BQ-123 (ET A receptor, 1 M) and BQ-788 (ET B receptor, 1 M), and the ETB receptor agonist sarafotoxin 6C (100 nM). ET-1 peptide levels were determined by ELISA, whereas ET A, ETB, and collagen levels were determined by Western blot analysis. ANG II increased ET-1 peptide levels in a timedependent manner. ANG II increased ET A and ETB receptor protein levels as well as collagen in a similar fashion. ANG II-induced collagen was reduced while in the presence of BQ-123, suggesting a role for the ET A receptor in the regulation of the extracellular matrix. ANG II treatment in the presence of BQ-788 significantly increased ET-1 peptide levels. Conversely, the ET B receptor agonist sarafotoxin 6C significantly decreased ET-1 peptide levels. These data implicate a role for the ET B receptor in the clearance of the ET-1 peptide. In conclusion, both ET A and ETB receptors are expressed in adventitial fibroblasts, which paves the ground for the biological significance of adventitial ET-1. The ET A receptor subtype mediates collagen I expression, whereas the ET B receptor subtype may play a protective role through increasing the clearance of the ET-1 peptide. adventia; endothelin-1; endothelin type A receptor; endothelin type B receptor THE ROLE OF ANG II in the development of hypertension and atherosclerosis has been well accepted. It has been demonstrated that endothelin (ET)-1 can contribute to many ANG II-mediated responses (8).The vascular effects of ET-1 are mediated through both ET A and ET B receptor subtypes. Specifically, the ET A receptor, located on vascular smooth muscle cells (VSMCs), has been shown to mediate vasoconstriction, the formation of ROS (21), and collagen accumulation (11). The role of the ET B receptor is associated with nitric oxide (NO)-mediated vasodilation in endothelial cells (ECs) (14) and vasoconstriction in VSMCs (36). Moreover, a report (12) has also suggest that the ET B receptor is responsible for the clearance of ET-1, a mechanism thought to keep ET-1 peptide levels low.Recent evidence has suggested that the adventitia of the blood vessel is a dynamic tissue (13). The adventitia has been identified as a major producer of ROS (38), reacting to vasoactive hormones such as ANG II (29). Moreover, local production of ET-1 within the adventitia has been r...

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“…Cross-sections of the BA ( ∼ 100 μm thick) were cut using a vibratome (Technical Product International, Inc., St. Louis, Mo., USA), collected in PBS and processed for EM-I of ET-1, ET A and SYP [Loesch et al, 2005;Steele et al, 2006;Loesch et al, 2010a]. Briefly, sections were exposed to 0.3% hydrogen peroxide in 33% methanol for 45 min (in order to block endogenous peroxidases), washed in PBS and then for ET-1 detection placed for 1 h in a 10% nonimmune normal goat serum (NGS; Nordic Immunology, Tilburg, The Netherlands), while for the detection of ET A and SYP sections were placed in a 10% nonimmune normal horse serum (NHS; Jackson ImmunoResearch Laboratories, West-Grave, Pa., USA).…”

Section: Em-i Of Et-1 Et a And Sypmentioning

confidence: 99%

“…Less is known regarding the presence and role of ET-1 and its receptors in cerebrovascular nerves [Loesch et al, 1998[Loesch et al, , 2005. So far, the presence of ET-1-positive cerebrovascular autonomic nerves has been reported in the BA of the rat [Loesch et al, 1998;Milner et al, 2000] and of the human middle cerebral artery [Loesch and Burnstock, 2002;Mickey et al, 2002].…”

mentioning

confidence: 99%

“…So far, the presence of ET-1-positive cerebrovascular autonomic nerves has been reported in the BA of the rat [Loesch et al, 1998;Milner et al, 2000] and of the human middle cerebral artery [Loesch and Burnstock, 2002;Mickey et al, 2002]. In the capybara BA, the presence of the endothelin A (ET A ) and B (ET B ) receptors was reported in the artery including its adventitial perivascular nerves [Loesch et al, 2005]. However, the presence of ET-1 in the perivascular nerves in capybara BA has thus far not been described.…”

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confidence: 99%

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Immunoreactive Endothelin-1 and Endothelin A Receptor in Basilar Artery Perivascular Nerves of Young and Adult Capybaras

Loesch

Dashwood²,

Ribeiro³

2013

Cells Tissues Organs

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The purpose of this qualitative morphological study was the immunocytochemical and ultrastructural comparison of perivascular nerves of the basilar artery (BA) of young (6-month-old) and adult (12-month-old) capybaras - adult capybaras showed regression of the internal carotid artery (ICA). The study focused on immunolabeling for the vasoactive peptide endothelin-1 (ET-1) and endothelin A receptor (ET_A) as well as for the synapse marker synaptophysin (SYP). In the BA of young capybaras, immunoreactivity for ET-1, ET_A receptor and SYP was detected in perivascular nerve varicosities and/or intervaricosities. Immunoreactivity for ET-1 and ET_A receptor was also displayed by some Schwann cells, which accompanied perivascular nerves. In addition to the presence of the above-described perivascular nerve characteristics, the BA of adult animals also revealed structurally altered perivascular nerves, where axon profiles were irregular in shape with dense axoplasm, while the cytoplasm of Schwann cells was vacuolated and contained myelin-like figures. These structurally altered perivascular nerves displayed immunoreactivity for ET-1, ET_A receptor and SYP. These results show that the ET-1 system is present in some of the BA perivascular nerves and it is likely that this system is affected during animal maturation when ICA regression takes place. The role of ET-1 in cerebrovascular nerves is still unclear but its involvement in neural (sensory) control of cerebral blood flow and nerve function is possible.

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Endothelin-1 and endothelin receptors in the basilar artery of the capybara

Cited by 9 publications

References 41 publications

Intracellular Endothelin Type B Receptor-driven Ca2+ Signal Elicits Nitric Oxide Production in Endothelial Cells

Intracellular Endothelin Type B Receptor-driven Ca2+ Signal Elicits Nitric Oxide Production in Endothelial Cells

ET_A and ET_B receptors are expressed in vascular adventitial fibroblasts

Immunoreactive Endothelin-1 and Endothelin A Receptor in Basilar Artery Perivascular Nerves of Young and Adult Capybaras

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Endothelin-1 and endothelin receptors in the basilar artery of the capybara

Cited by 9 publications

References 41 publications

Intracellular Endothelin Type B Receptor-driven Ca2+ Signal Elicits Nitric Oxide Production in Endothelial Cells

Intracellular Endothelin Type B Receptor-driven Ca2+ Signal Elicits Nitric Oxide Production in Endothelial Cells

ETA and ETB receptors are expressed in vascular adventitial fibroblasts

Immunoreactive Endothelin-1 and Endothelin A Receptor in Basilar Artery Perivascular Nerves of Young and Adult Capybaras

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ET_A and ET_B receptors are expressed in vascular adventitial fibroblasts