Endothelin-1 as a Local Ovarian Regulator in Porcine Granulosa Cells

Kubota, Toshiro; Kamada, Shinji; Asô, Takeshi

doi:10.1159/000183940

Cited by 13 publications

(12 citation statements)

References 12 publications

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“…ET-1 produced by these cells may function as an autocrine/paracrine growth factor and modulator of steroidogenesis [Kamada et al, 1993]. Positive ET-1 immunoreactivity and Northern blot expression of mRNA for prepro-ET-1 in cultured porcine granulosa cells also suggest that these substances exert autocrine/paracrine effects on the function of granulosa cells and that ET-1 acts as modulator of steroid metabolism in preovulatory follicles [Kubota et al, 1994]. Autoradiographic studies have shown that in the pig granulosa cells contain ET receptors and that they are also distributed in the vascular components of the ovary [Flores et al, 1995].…”

Section: Discussionmentioning

confidence: 99%

Endothelin-1 and Endothelial Nitric Oxide Synthase Immunoreactivity in Lymphatic Vessels of the Uterine Broad Ligament during the Estrous Cycle in the Pig

Doboszyńska

Andronowska

Modzelewska³

2002

Cells Tissues Organs

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Immunohistochemical localization and distribution of endothelin (ET-1) and nitric oxide synthase (eNOS) were investigated in precollector and collector lymphangions of lymphatic vessels leaving the ovary and were found in the vascular subovarian plexus (mesovarium) as well as in those emanating from the oviductal isthmus and uterine horn (mesosalpinx and mesometrium, respectively) forming the paraovarian lymphatic plexus in the broad ligament of the uterus during different phases of the estrous cycle in pigs. The polyclonal antibody for ET-1 and the monoclonal antibody for eNOS isoform were used for studies on the light-microscopic level. Immunoreactivities to both ET-1 and eNOS were observed in the endothelial cell cytoplasm of precollector and collector lymphangions and were not demonstrated in smooth muscle cells of the lymphatics examined. In the endothelium, the intensity of immunostaining for ET-1 and eNOS was found to be estrous phase-dependent and differed between precollector and collector lymphangions. In general, immunoreactivity to ET-1 was more intense in the endothelium of shrunken lymphangions, whereas that for eNOS was more intense in lymphangions with the large lumen. These results suggest that ET-1 and eNOS can play a role in mechanisms regulating the vascular contractile activity promoting lymph flow during the estrous cycle in the porcine broad ligament.

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Section: Discussionmentioning

confidence: 99%

Endothelin-1 and Endothelial Nitric Oxide Synthase Immunoreactivity in Lymphatic Vessels of the Uterine Broad Ligament during the Estrous Cycle in the Pig

Doboszyńska

Andronowska

Modzelewska³

2002

Cells Tissues Organs

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“…ET-1 could then act through ET B receptors on the capillary endothelial cells of the corpora lutea and through ET A receptors on the vascular smooth muscle cells in the media of stromal vessels. An autocrine action on granulosa cells was suggested by the presence of ET receptors (Kamada et al 1995(Kamada et al , 1992Kubota et al 1994;Tedeschi et al 1994), the synthesis of ET-1 (Iwai et al 1991;Kamada et al 1993a;Magini et al 1996) and ET-1 mRNAs (Kubota et al 1994;Magini et al 1996), and the expression of ECE-1.…”

Section: Discussionmentioning

confidence: 99%

“…Cultured porcine granulosa cell media contain ET-1 (Iwai et al 1991;Kamada et al 1993a). Cultured porcine granulosa cells express ET-1 mRNA (Kubota et al 1994). Human granulosa cells in situ would contain ET-1 peptide and mRNAs at different stages of maturation (Magini et al 1996).…”

Section: Introductionmentioning

confidence: 99%

“…Human granulosa cells in situ would contain ET-1 peptide and mRNAs at different stages of maturation (Magini et al 1996). Endothelin receptors, mainly of the ET B type, are present in porcine (Kamada et al 1992;Kubota et al 1994) and rat (Tedeschi et al 1994) granulosa cells. However, other authors (Kamada et al 1995) reported a different pattern of expression for ET-receptors in human luteinized granulosa cells, with a prominent expression of the ET B type over the ET A type.…”

Section: Introductionmentioning

confidence: 99%

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The endothelin system in human and monkey ovaries: in situ gene expression of the different components

Karam

Valdenaire

Bélair

et al. 1999

Cell and Tissue Research

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The endothelin system is composed of three endothelin isoforms (ET-1, ET-2, and ET-3), the endothelin receptors ETA and ETB, and the endothelin-converting enzyme (ECE). Besides having a major vasoactive role, endothelins have roles in different cell types at a local level. We investigated the presence of the different components of the endothelin system in primate ovaries. Human ovaries and gonadotropin-stimulated monkey ovaries were studied using immunohistochemistry for endothelin, and in situ hybridization with probes for ET-1, ET-2, ET-3, ETA and ETB receptors, and ECE. ET-1 and ETA receptors were detected in endothelial cells and vascular smooth muscle cells, respectively, in stromal vessels adjacent to follicles and corpora lutea. ETB receptors and ET-1 were found in the endothelial cells of capillaries of corpora lutea. ECE was present in internal theca cells of secondary, de Graaf, atretic follicles, and in luteinized granulosa cells of the corpora lutea. The endothelin system components are present in or around the follicles of human and monkey ovaries. Although the components are not expressed in the same cell types, they are synthesized, mainly in follicles, by cells that are in close proximity. Thus, the endothelin system could act in a paracrine manner. ECE expression in steroid-producing cells changes its compartmentalization during follicle maturation.

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“…In contrast, there have been several reports on the physiological roles of ET-1 in uterus and ovary. Expression of the ET-1 gene was detected in human and porcine granulosa cells (Kubota et al 1994, Magini et al 1996. The peptide is considered to play an important role in granulosa cell function.…”

Section: Tissue-restricted Expression Of the Vic/et-2 Genementioning

confidence: 99%

Gene expression of vasoactive intestinal contractor/endothelin-2 in ovary, uterus and embryo: comprehensive gene expression profiles of the endothelin ligand-receptor system revealed by semi-quantitative reverse transcription-polymerase chain reaction analysis in adult mouse tissues and during late embryonic development

Uchide

Masuda²,

Mitsui³

et al. 1999

Journal of Molecular Endocrinology

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Vasoactive intestinal contractor (VIC)/endothelin-2 (ET-2) is a 21 amino acid intestinal peptide characterized as a potent vasoactive and intestinal smooth muscle-contracting compound. To investigate the physiological roles of VIC/ET-2 further, we characterized the specificity of VIC gene expression relative to that of other members of the endothelin (ET) ligand-receptor system in adult mouse tissues and during embryonic development. Gene expression of ET-1, ET-3, ETA and ETB was ubiquitous in almost all tissues we examined while gene expression of VIC was localized to certain tissues. A high level of VIC gene expression was observed in ovary and uterus. The gene expression of VIC, relative to that of glyceraldehyde-3-phosphate dehydrogenase, was approximately 2·0%, 0·4%, and 2·3% in ovary, uterus, and intestine respectively, and was approximately 1·6 and 7·1 times higher than that of ET-1 in ovary and intestine respectively. Thus, VIC may have some physiological role in adult ovary and uterus as well as intestine. In embryonic development, VIC gene expression sharply increased between 11 and 15 days post coitus and decreased after birth, suggesting an involvement in the later stages of embryonic development.

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Endothelin-1 as a Local Ovarian Regulator in Porcine Granulosa Cells

Cited by 13 publications

References 12 publications

Endothelin-1 and Endothelial Nitric Oxide Synthase Immunoreactivity in Lymphatic Vessels of the Uterine Broad Ligament during the Estrous Cycle in the Pig

Endothelin-1 and Endothelial Nitric Oxide Synthase Immunoreactivity in Lymphatic Vessels of the Uterine Broad Ligament during the Estrous Cycle in the Pig

The endothelin system in human and monkey ovaries: in situ gene expression of the different components

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