Endothelin-1 in exhaled breath condensate of allergic asthma patients with exercise-induced bronchoconstriction

Ziętkowski, Ziemowit; Skiepko, Roman; Tomasiak, Maria Magdalena; Bodzenta-Łukaszyk, Anna

doi:10.1186/1465-9921-8-76

Cited by 31 publications

(18 citation statements)

References 32 publications

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“…There are a few reports also suggesting that EIB, through the release of many pro-inflammatory cytokines and activation of inflammatory cells, could augment airway inflammation and bronchial hyperreactivity [19,20]. …”

Section: Discussionmentioning

confidence: 99%

Changes in High-Sensitivity C-Reactive Protein in Serum and Exhaled Breath Condensate after Intensive Exercise in Patients with Allergic Asthma

Ziętkowski¹,

Skiepko²,

Tomasiak-Lozowska³

et al. 2010

Int Arch Allergy Immunol

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Background: Exercise-induced bronchoconstriction (EIB) in asthmatics depends on the presence of allergic inflammation. This study was performed to assess the possible association of EIB with low-grade systemic inflammation, whose presence was revealed in asthmatic patients. Methods: The study was conducted in a group of 24 asthmatics (14 with EIB, 10 without EIB) and 8 healthy volunteers. Changes in serum and exhaled breath condensate (EBC) high-sensitivity C-reactive protein (hs-CRP) levels induced by intensive exercise were determined. Moreover, the possible correlation of these measurements with the results of other tests used in the diagnosis of asthma as well as laboratory tests commonly associated with asthma were investigated. Results: In asthmatic patients with EIB, a statistically significant increase in hs-CRP levels both in serum and EBC after an exercise test was observed. Twenty-four hours after the exercise test in the group of asthmatics with EIB, a statistically significant increase in exhaled nitric oxide (F_ENO), serum eosinophil cationic protein (ECP) concentrations and bronchial hyperreactivity to histamine was revealed. A statistically significant correlation between the maximum increase in hs-CRP levels both in serum and EBC after exercise and either baseline F_ENO and an increase in serum ECP or F_ENO 24 h after exercise in the group of asthmatics with EIB was revealed. Conclusions: We show that, as a result of intensive exercise leading to bronchoconstriction, an increase in serum and EBC hs-CRP occurs. Our observations could suggest that in asthmatic patients, as a consequence of exercise-induced bronchoconstriction, an intensification of low-grade systemic inflammation can be observed.

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Section: Discussionmentioning

confidence: 99%

Changes in High-Sensitivity C-Reactive Protein in Serum and Exhaled Breath Condensate after Intensive Exercise in Patients with Allergic Asthma

Ziętkowski¹,

Skiepko²,

Tomasiak-Lozowska³

et al. 2010

Int Arch Allergy Immunol

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“…Exhaled breath condensate has been used in various studies to evaluate indicators specific to the airway diseases in children and has been proven to be reliable (6). Analysis of endothelin and PDGF in the condensed breathing air (EBC) is the way of determining the existing damage by these effective mediators and it is a clinically valuable method (5,7). There is a small number of studies evaluating the ventricle functions by Tissue Doppler imaging (TDI) in asthmatic children.…”

Section: Introductionmentioning

confidence: 99%

Relation between Vascular Endothelial Markers and Right Ventricular Function in the Children with Asthma

et al. 2017

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Objectives: The aim of the study was to evaluate the possible relationship of clinical severity during asthmatic exacerbation with the ventricular functions and the levels of vascular endothelial-related biomarkers endothelin-1 (ET-1) and platelet-derived growth factor-BB (PDGF-BB) in the condensed breathing air in the children with asthma. Methods: The study included a total of 80 children with acute asthmatic episode; of these, 28 had a mild, 26 had a moderate, and 26 had a severe attack. Samples of condensed breathing air were obtained for being analyzed for ET-1 and PDGF-BB levels during the study. All patients were evaluated by echocardiography at the beginning of the study and two weeks after the treatment of asthmatic episode. Results: Before treatment, mPAP in moderate and severe asthma groups was significantly higher than in mild asthma group (21.6 ± 7.3, 30.1 ± 9.8 and 32.7 ± 7.8; P < 0.01, P < 0.001, respectively). After treatment, no significant difference was detected between the asthma groups (P > 0.05). Am and Em waves, and IVRT, IVCT, ET and RV MPI during the asthmatic attack and after treatment did not change significantly between the groups; pre-and post-treatment values of these parameters also did not show significant differences. In exhaled air samples of the group with severe asthma exacerbation ET-1 and PDGF-BB were higher than those in the moderate and mild group (0.80 ± 0.42, 0.58 ± 0.30, 0.50 ± 0.15; P = 0.002 and 281.7 ± 253.2, 167.3 ± 148.1, 135.9 ± 74.9; P = 0.008 respectively). Conclusions: Our study results showed that mPAP in direct proportion with the increased inflammatory cytokines was significantly higher in asthma attacks and levels of ET-1 and PDGF-BB were significantly increased with severity of asthma, indicating a vascular response.

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“…Carraro et al [32] noted higher EBC Cys-LT levels in asthmatic children with EIB, which correlated with the decrease in FEV 1 after exercise. We also found a significant increase in endothelin-1 levels in EBC collected between 10 min and 6 h after exercise [33]. …”

Section: Discussionmentioning

confidence: 99%

RANTES in Exhaled Breath Condensate of Allergic Asthma Patients with Exercise-Induced Bronchoconstriction

Ziętkowski¹,

Skiepko²,

Tomasiak-Lozowska³

et al. 2009

Respiration

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Background: The response of asthmatics to exercise differs from that of healthy subjects, and the mechanisms responsible for exercise-induced bronchoconstriction (EIB) remain to be elucidated. Objectives: The aim of this study was to evaluate changes in RANTES levels in exhaled breath condensate (EBC) following intensive exercise in allergic asthmatics. Methods: The study was conducted in a group of 19 asthmatics (11 with EIB and 8 without EIB) and 7 healthy volunteers. Changes in the concentrations of RANTES in EBC induced during the 24 h after intensive exercise were determined. Moreover, these measurements were tested for possible correlations with the results of other tests commonly associated with asthma as well as with changes in airway inflammation after exercise. Results: In contrast to asthmatic patients without EIB and healthy controls, in asthmatics with EIB RANTES concentrations were statistically significantly increased in EBC collected during the first 24 h after an exercise test. There was a statistically significant correlation between the maximum increase in RANTES concentrations in EBC after exercise and either baseline exhaled nitric oxide (F_ENO) or bronchial hyperreactivity to histamine and an increase in serum eosinophil cationic protein or F_ENO 24 h after exercise in the EIB asthmatics. Conclusions: The increase in RANTES in asthmatic airways, promoting the migration and activation of inflammatory cells including eosinophils, may play an important role in the upregulation of airway inflammation after EIB in asthmatic patients.

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Endothelin-1 in exhaled breath condensate of allergic asthma patients with exercise-induced bronchoconstriction

Cited by 31 publications

References 32 publications

Changes in High-Sensitivity C-Reactive Protein in Serum and Exhaled Breath Condensate after Intensive Exercise in Patients with Allergic Asthma

Changes in High-Sensitivity C-Reactive Protein in Serum and Exhaled Breath Condensate after Intensive Exercise in Patients with Allergic Asthma

Relation between Vascular Endothelial Markers and Right Ventricular Function in the Children with Asthma

RANTES in Exhaled Breath Condensate of Allergic Asthma Patients with Exercise-Induced Bronchoconstriction

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