Endothelin-Converting Enzyme 1 and Vascular Endothelial Growth Factor as Potential Biomarkers during Ex Vivo Lung Perfusion with Prolonged Hypothermic Lung-Sparing

Hernández-Jiménez, Claudia; Olmos-Zuñiga, J. Raúl; Baltazares-Lipp, Matilde; Jasso-Victoria, Rogelio; Polo-Jerez, Adrián; Pérez-López, María; Vázquez-Justiniano, Luis Florentino; Díaz-Martínez, N.E.; Gaxiola-Gaxiola, Miguel; Romero-Romero, Laura; Guzmán-Cedillo, Axel Edmundo; Baltazares-Lipp, Mario Enrique; Vázquez-Minero, Juan Carlos; Gutiérrez‐González, Luis H.; Alonso-Gómez, Marcelino; Silva-Martínez, Mariana

doi:10.1155/2022/6412238

Cited by 4 publications

(1 citation statement)

References 38 publications

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“…Its malfunction within the walls of major arteries is an important contributor to the local and systemic signs of poor endothelial functions in health. However, in sickness, it promotes the normal functioning of many tissues and organs of the body (Hernández‐Jiménez et al, 2022; Sanusi et al, 2020). Increased oxidative/nitrosative stress, up‐regulation of inflammatory activity, and, most importantly, defective endothelin/endothelial nitric oxide synthase (eNOS) signaling have all been documented as precursors of endothelial dysfunction (Asiwe, Kolawole, et al, 2022; Jasińska‐Stroschein, 2022; Virk et al, 2021).…”

Section: Introductionmentioning

confidence: 99%

Treatment with Ginkgo biloba supplement modulates oxidative disturbances, inflammation and vascular functions in oxygen deprived hypothyroid mice: Involvement of endothelin‐1/NO signaling pathways

Adebayo¹,

Aduema

Iwueke

et al. 2022

Journal of Food Biochemistry

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A double‐hit biological alteration involving exposure to oxygen deprivation in hypothyroid condition may exacerbate cellular oxidative and inflammatory disturbances comparative to a one‐hit biological exposure. This study investigated the therapeutic effect of Ginkgo biloba as cardioprotective against aortic oxido‐inflammatory disturbances following oxygen deprivation in hypothyroid mice. Male Swiss mice were partitioned into 5 groups (n = 6) for hypothyroidism (Carbimazole 1.2 mg/kg) and hypoxia induction. Group 1 (normal control), group 2 (hypoxic stress control), group 3 (hypoxic and hypothyroid stress), group 4 (hypoxic and hypothyroid stress and Ginkgo biloba 20 mg/kg; p.o) and group 5 (hypoxic and hypothyroid stress and Levothyroxine 10 μg/kg; p.o) for 14 days. Thereafter, serum and aorta was collected for biochemical evaluation. GBS did not up‐regulate the serum thyroid hormone imbalances (tri‐iodothyronine (T3), thyroxin (T4)) but maintains the TSH levels. The blood glucose level was reduced with decrease oxidative stress and inflammatory mediators in the serum/aorta indicated by inhibited redox status following treatment with GBS. Moreover, endothelin‐1/nitric oxide signaling pathways were markedly regulated in the aorta. Conclusively, GBS acts as a therapeutic agent and may be consider as a potential vasodilator candidate in the management and control of hypoxic stress in hypothyroid condition. Practical applications Treatment with Gingko biloba supplement abated endothelial abnormalities via elevation of nitric oxide release and suppression of endothelin activity in hypothyroid mice exposed to hypoxic hypoxia. The activity of myeloperoxidase enzyme and redo‐inflammatory status was downregulated following treatment with Gingko biloba supplement in hypothyroid mice exposed to hypoxic hypoxia. Treatment with Gingko biloba supplement modulates hypothalamic–pituitary–adrenal (HPA) axis by inhibiting corticosterone release in hypothyroid mice exposed to hypoxic hypoxia.

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Section: Introductionmentioning

confidence: 99%

Treatment with Ginkgo biloba supplement modulates oxidative disturbances, inflammation and vascular functions in oxygen deprived hypothyroid mice: Involvement of endothelin‐1/NO signaling pathways

Adebayo¹,

Aduema

Iwueke

et al. 2022

Journal of Food Biochemistry

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Exploring predisposing factors and pathogenesis contributing to injuries of donor lungs

Zhang

et al. 2022

Expert Review of Respiratory Medicine

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Impact of Hypothermic Perfusion on Immune Responses and Sterile Inflammation in a Preclinical Model of Pancreatic Transplantation

Mesnard,

Bruneau,

Le Bas-Bernardet

et al. 2024

Transplantation Direct

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Background. In organ transplantation, cold ischemia is associated with sterile inflammation that subsequently conditions adaptive immunity directed against the grafts during revascularization. This inflammation is responsible for venous thrombosis, which is the main postoperative complication affecting graft function. Our aim was to investigate the modulation of immune responses and endothelial function of pancreatic grafts during cold ischemia using different preservation modalities. Methods. According to a preclinical porcine model of controlled donation after circulatory death, pancreatic grafts were preserved under hypothermic conditions for 24 h according to 4 modalities: static cold storage, hypothermic machine perfusion, hypothermic oxygenated perfusion at 21%, and 100%. Biopsies of the head and tail of the pancreas were performed during preservation. The first step involved a broad screening of the gene expression profile (84 genes) during preservation on a limited number of grafts. In the second step, a confirmation test was performed in all 4 groups. Results. Vascular endothelial growth factor gene expression showed a decrease during preservation in the hypothermic oxygenated perfusion 21% and 100% groups compared with the static cold storage group. In contrast, thrombomodulin gene expression showed an increase during preservation in the hypothermic oxygenated perfusion 21% and 100% groups compared with the static cold storage and hypothermic machine perfusion groups. Conclusions. We demonstrated that compared with static cold storage, hypothermic oxygenated perfusion is an effective modality for modulating endothelial function by increasing thrombomodulin expression and decreasing ischemia and vascular endothelial growth factor expression.

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Endothelin-Converting Enzyme 1 and Vascular Endothelial Growth Factor as Potential Biomarkers during Ex Vivo Lung Perfusion with Prolonged Hypothermic Lung-Sparing

Cited by 4 publications

References 38 publications

Treatment with Ginkgo biloba supplement modulates oxidative disturbances, inflammation and vascular functions in oxygen deprived hypothyroid mice: Involvement of endothelin‐1/NO signaling pathways

Treatment with Ginkgo biloba supplement modulates oxidative disturbances, inflammation and vascular functions in oxygen deprived hypothyroid mice: Involvement of endothelin‐1/NO signaling pathways

Exploring predisposing factors and pathogenesis contributing to injuries of donor lungs

Impact of Hypothermic Perfusion on Immune Responses and Sterile Inflammation in a Preclinical Model of Pancreatic Transplantation

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